Transcriptional and Proliferative Heterogeneity Among Leukemia Cell Lines: A Baseline Characterization of Epigenetic and DNA-Repair Pathways

Ecmel Mehmetbeyoğlu Duman; Ayse Uz; Sudenur Sancak; Mai R. S. Abusalim; Venhar Çınar; Serpil Taheri

Transcriptional and Proliferative Heterogeneity Among Leukemia Cell Lines: A Baseline Characterization of Epigenetic and DNA-Repair Pathways

Abstract

Leukemia comprises biologically diverse hematologic malignancies with subtype-specific molecular characteristics that influence disease behavior and therapeutic response. To establish baseline transcriptional and proliferative profiles across representative leukemia models, we quantified the expression of TRDMT1 (DNMT2), RAD51, TET2, and XPA in six leukemia cell lines (ARH-77, CCRF-SB, HL-60, K-562, Kasumi-1, SUP-T1) and assessed their growth dynamics over a 96-hour period. qPCR analysis revealed significant differences in the baseline expression of all four genes among cell lines. ARH-77 showed the highest expression levels for each gene, while HL-60 and K-562 generally displayed lower expression. Growth assays demonstrated variable proliferation rates, with SUP-T1 and CCRF-SB exhibiting the greatest increases in cell number. To contextualize these findings within clinical disease patterns, expression of the same genes was examined in hematologic malignancies using the BloodSpot dataset (GSE13159). Public transcriptomic data demonstrated heterogeneous subtype-dependent expression ranges for TRDMT1, RAD51, TET2, and XPA, and these patterns aligned with the variability observed across the leukemia cell lines. Together, these results provide a comparative molecular and phenotypic baseline for commonly used leukemia models and establish a reference framework for subsequent functional and therapeutic studies.

Keywords

Supporting Institution

This project funded by Health Institutes of Türkiye (TUSEB) with the project code 2025-A4-01-43829.

Project Number

This project funded by Health Institutes of Türkiye (TUSEB) with the project code 2025-A4-01-43829.

Ethical Statement

This study does not require ethics committee approval.

Thanks

We thank to Dr. Mustafa Burak Acar for gifting the cell lines that are used in this study.

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Details

Primary Language

English

Subjects

Cancer Biology

Journal Section

Research Article

Authors

Ecmel Mehmetbeyoğlu Duman
0000-0002-4597-6811
Türkiye

Ayse Uz
0009-0004-7167-1909
Türkiye

Sudenur Sancak
0009-0008-5811-1762
Türkiye

Mai R. S. Abusalim
0000-0002-2778-0380
Türkiye

Venhar Çınar
0000-0003-1544-8994
Türkiye

Serpil Taheri ^*
0000-0001-5806-8241
Türkiye

Publication Date

December 30, 2025

Submission Date

November 14, 2025

Acceptance Date

December 24, 2025

Published in Issue

Year 2025 Number: 1

IZ

https://izlik.org/JA48SG32ES

Cite

RIS / Bibtex

APA

Mehmetbeyoğlu Duman, E., Uz, A., Sancak, S., R. S. Abusalim, M., Çınar, V., & Taheri, S. (2025). Transcriptional and Proliferative Heterogeneity Among Leukemia Cell Lines: A Baseline Characterization of Epigenetic and DNA-Repair Pathways. Erciyes BioGenome, 1, 11-21. https://izlik.org/JA48SG32ES

AMA

1.Mehmetbeyoğlu Duman E, Uz A, Sancak S, R. S. Abusalim M, Çınar V, Taheri S. Transcriptional and Proliferative Heterogeneity Among Leukemia Cell Lines: A Baseline Characterization of Epigenetic and DNA-Repair Pathways. Erciyes BioGenome. 2025;(1):11-21. https://izlik.org/JA48SG32ES

Chicago

Mehmetbeyoğlu Duman, Ecmel, Ayse Uz, Sudenur Sancak, Mai R. S. Abusalim, Venhar Çınar, and Serpil Taheri. 2025. “Transcriptional and Proliferative Heterogeneity Among Leukemia Cell Lines: A Baseline Characterization of Epigenetic and DNA-Repair Pathways”. Erciyes BioGenome, no. 1: 11-21. https://izlik.org/JA48SG32ES.

EndNote

Mehmetbeyoğlu Duman E, Uz A, Sancak S, R. S. Abusalim M, Çınar V, Taheri S (December 1, 2025) Transcriptional and Proliferative Heterogeneity Among Leukemia Cell Lines: A Baseline Characterization of Epigenetic and DNA-Repair Pathways. Erciyes BioGenome 1 11–21.

IEEE

[1]E. Mehmetbeyoğlu Duman, A. Uz, S. Sancak, M. R. S. Abusalim, V. Çınar, and S. Taheri, “Transcriptional and Proliferative Heterogeneity Among Leukemia Cell Lines: A Baseline Characterization of Epigenetic and DNA-Repair Pathways”, Erciyes BioGenome, no. 1, pp. 11–21, Dec. 2025, [Online]. Available: https://izlik.org/JA48SG32ES

ISNAD

Mehmetbeyoğlu Duman, Ecmel - Uz, Ayse - Sancak, Sudenur - R. S. Abusalim, Mai - Çınar, Venhar - Taheri, Serpil. “Transcriptional and Proliferative Heterogeneity Among Leukemia Cell Lines: A Baseline Characterization of Epigenetic and DNA-Repair Pathways”. Erciyes BioGenome. 1 (December 1, 2025): 11-21. https://izlik.org/JA48SG32ES.

JAMA

1.Mehmetbeyoğlu Duman E, Uz A, Sancak S, R. S. Abusalim M, Çınar V, Taheri S. Transcriptional and Proliferative Heterogeneity Among Leukemia Cell Lines: A Baseline Characterization of Epigenetic and DNA-Repair Pathways. Erciyes BioGenome. 2025;:11–21.

MLA

Mehmetbeyoğlu Duman, Ecmel, et al. “Transcriptional and Proliferative Heterogeneity Among Leukemia Cell Lines: A Baseline Characterization of Epigenetic and DNA-Repair Pathways”. Erciyes BioGenome, no. 1, Dec. 2025, pp. 11-21, https://izlik.org/JA48SG32ES.

Vancouver

1.Ecmel Mehmetbeyoğlu Duman, Ayse Uz, Sudenur Sancak, Mai R. S. Abusalim, Venhar Çınar, Serpil Taheri. Transcriptional and Proliferative Heterogeneity Among Leukemia Cell Lines: A Baseline Characterization of Epigenetic and DNA-Repair Pathways. Erciyes BioGenome [Internet]. 2025 Dec. 1;(1):11-2. Available from: https://izlik.org/JA48SG32ES