In food safety control overview of using Real-Time PCR
Yıl 2022,
, 53 - 60, 31.12.2022
Erdem Artuvan
,
Salih Aksay
Öz
The identity-determining importance of nucleic acids in living beings is a guide for reaching the desired information about food in quality control analyzes. With these goals, the popularity of Real-time PCR (Polymerase Chain Reaction) methods, which are one of the nucleic acid-based methods, is rapidly increasing due to their high reproducibility, precision and fast result production. It is thought that informative studies about the analysis using the device will provide a basis for researches on the subject. This review contains information about the studies conducted on Real-Time PCR analysis used to detect imitation / adulteration and cheating in foods. General descriptions about the operation of the Real-Time PCR methods are given. The quality control analyzes in which the method was used were classified and explanations were made about each analysis area and examples from the studies in the literature were given.
Kaynakça
- Agrimonti, C., Bottari, B., Sardaro, M. L., & Marmiroli, N. (2019). Application of real-time PCR (qPCR) for characterization of microbial populations and type of milk in dairy food products. Critical Reviews in Food Science and Nutrition, 59(3), 423-442. https://doi.org/10.1080/10408398.2017.1375893
- Agrimonti, C., Pirondini, A., Marmiroli, M., & Marmiroli, N. (2015). A quadruplex PCR (qxPCR) assay for adulteration in dairy products. Food Chemistry, 187, 58-64. https://doi.org/10.1016/j.foodchem.2015.04.017
- Ahmed, F. E. (2002). Detection of genetically modified organisms in foods. Trends in Biotechnology, 20(5), 215-223. https://doi.org/10.1016/S0167-7799(01)01920-5
- Alary, R., Serin, A., Maury, D., Jouira, H. B., Sirven, J.-P., Gautier, M.-F., & Joudrier, P. (2002). Comparison of simplex and duplex real-time PCR for the quantification of GMO in maize and soybean. Food Control, 13, 235-244. https://doi.org/10.1016/S0956-7135(02)00015-4
- Ali, M. E., Hashim, U., Dhahi, S. T., Mustafa, S., Man, Y., & Latif, M. A. (2012). Analysis of Pork Adulteration in Commercial Burgers Targeting Porcine-Specific Mitochondrial Cytochrome B Gene by TaqMan Probe Real-Time Polymerase Chain Reaction. Food Analytical Methods, 5, 784-794. http://dx.doi.org/10.1007/s12161-011-9311-4
- Alikord, M., Momtaz, H., Keramat, J., Kadivar, M., & Rad, A. H. (2018). Species identification and animal authentication in meat products: a review. Journal of Food Measurement and Characterization, 12, 145-155. https://doi.org/10.1007/s11694-017-9625-z
- Bottero, M., Civera, T., Nucera, D., Rosati, P., Sacchi, P., & Turi, R. (2003). A multiplex polymerase chain reaction for the identification of cows', goats' and sheep's milk in dairy products. International Dairy Journal, 13, 277-282. http://dx.doi.org/10.1016/S0958-6946(02)00170-X
- Böhme, K., Calo-Mata, P., Barros-Velazquez, J., & Ortea, I. (2019). Review of Recent DNA-Based Methods for Main Food-Authentication Topics. Journal of Agricultural and Food Chemistry, 67, 3854-3864. https://doi.org/10.1021/acs.jafc.8b07016
- Corman, V. M., Landt, O., Kaiser, M., Molenkamp, R., Meijer, A., Chu, D. K., Drosten, C. (2020). Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR. Eurosurveillance, 25(3), 1-8. https://dx.doi.org/10.2807%2F1560-7917.ES.2020.25.3.2000045
- Deniz, H. İ., & Kılıç Altun, S. (2017). The Isolation and Identification of Virulent Listeria monocytogenes Consumption of Local Cheeses in Şanlıurfa Province. Ataturk University Journal of Veterinary Sciences, 12(3), 289-295. http://dx.doi.org/10.17094/ataunivbd.368984
- Di Pinto, A., Terio, V., Marchetti, P., Bottaro, M., Mottola, A., Bozzo, G., Tantillo, G. (2017). DNA-based approach for species identificaton of goat-milk products. Food Chemistry, 229, 93-97. https://doi.org/10.1016/j.foodchem.2017.02.067
- Fagan, J. (2004). DNA Based Methods for Detection and Quantification of GMOs: Principles and Standards. Testing of Genetically Modified Organisms in Foods (s. 163). In New York: Food Products Press.
- Fang, X., & Zhang, C. (2016). Detection of adulterated murine components in meat products by TaqMan real-time PCR. Food Chemistry, 192, 485-490. https://doi.org/10.1016/j.foodchem.2015.07.020
- Ferreira, T., Farah, A., Oliveira, T. C., Lima, I. S., Vitorio, F., & Oliveira, E. M. (2016). Using Real-Time PCR as a tool for monitoring the authenticity of commercial coffees. Food Chemistry, 199, 433-438. https://doi.org/10.1016/j.foodchem.2015.12.045
- Gansbeke, B., Bény, G., De Loose, M., & Taverniers, I. (2018). A TaqMan Real-Time PCR Assay for Apricot (Prunus armeniaca) as an Authenticity Test for Detection of Traces of Persipan in Marzipan. Food Anaytical Methods, 11, 62-68. https://link.springer.com/article/10.1007/s12161-017-0964-5
- Garber, E., Parker, C., Handy, S., Cho, C., Panda, R., Samadpour, M., Ziobro, G. (2016). Presence of Undeclared Food Allergens in Cumin: The Need for Multiplex Methods. Journal of Agricultural and Food Chemistry, 64, 1202-1211. https://doi.org/10.1021/acs.jafc.5b05497
- Gerdes, L., Busch, U., & Pecoraro, S. (2012). Parallelised real-time PCR for identification of maize GMO events. European Food Research and Technology, 234, 315-322. http://dx.doi.org/10.1007%2Fs00217-011-1634-2
- Halkman, K. (2019). Microorganisms in Food. Food Microbiology (p. 309-404). In Ankara: Başak Publishing House.
- Hernandes, M., Ferrando, A., & Rodriguez-Lazaro, D. (2010). Assessment of Genetically Modifi ed Organisms (GMO) in Meat Products by PCR. Handbook of Meat Processing (s. 501-518). In Singapore: Blackwell Publishing. http://dx.doi.org/10.1002/9780813820897.ch29
- Herrero, B., Vieites, J. M., & Espineira, M. (2012). Fast Real-Time PCR for the Detection of Crustacean Allergen in Foods. Journal of Agricultural and Food Chemistry, 60, 1893-1897. https://doi.org/10.1021/jf2043532
- Herrero, B., Vieites, J. M., & Espineira, M. (2014). Development of an in-house fast real-time PCR method for detection of fish allergen in foods and comparison with a commercial kit. Food Chemistry, 151, 415-420. https://doi.org/10.1016/j.foodchem.2013.11.042
- Higuchi, R., Fockler, C., Dollinger, G., & Watson, R. (1993). Kinetic PCR Analysis: Real-time Monitoring of DNA Amplification Reactions. Nature Biotechnology, 11, 1026-1030. https://doi.org/10.1038/nbt0993-1026
- Holland, P. M., Abramson, R. D., Watson, R., & Gelfand, D. H. (1991). Detection of specific polymerase chain reaction product by utilizing the 5'----3' exonuclease activity of Thermus aquaticus DNA polymerase. Proceedings of the National Academy of Sciences of the United States of America, 88(16), 7276-7280. https://dx.doi.org/10.1073%2Fpnas.88.16.7276
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- Iwobi, A., Sebah, D., Kraemer, I., Losher, C., Fischer, G., Busch, U., & Huber, I. (2015). A multiplex real-time PCR method for the quantification of beef and pork fractions in minced meat. Food Chemistry, 169, 305-313. https://doi.org/10.1016/j.foodchem.2014.07.139
- Iwobi, A., Sebah, D., Spielmann, G., Maggipinto, M., Schrempp, M., Kraemer, I., Huber, I. (2017). A multiplex real-time PCR method for the quantitative determination of equine (horse) fractions in meat products. Food Control, 74, 89-97. https://doi.org/10.1016/j.foodcont.2016.11.035
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- Kabacaoğlu, E., & Karakaş Budak, B. (2019). Detection and quantification of salep with real time PCR utilizing the nr-ITS2 region. Journal of the Science of Food and Agriculture, 99, 2447-2454. https://doi.org/10.1002/jsfa.9453
- Kara, R., & Demirel, Y. N. (2016). Determination of Milk Species Used in Afyon Kaymak Production with RealTime PCR. Ataturk University Journal of Veterinary Sciences, 11(2), 185-190. http://dx.doi.org/10.17094/avbd.77186
- Karakılıç, M., Suna, S., Tamer, C. E., & Çopur, Ö. U. (2014). Food Allergy Reactions and Their Formation Mechanism. Journal of Agricultural Faculty of Uludag University, 28(1), 73-82.
- Kaya, M., Arıkan Asan, A., & Başıaçık Karakoç, Ş. (2019). Determination The Proportion of Chicken and Beef In Meat Products Using Some Commercial Kits. Süleyman Demirel University Journal of Natural and Applied Sciences, 23(1), 52-58. https://doi.org/10.19113/sdufenbed.442199
- Kesmen, Z., Büyükkiraz, M. E., Kahraman, N., & Yetim, H. (2017). Detection of Fraudulent Practices Involving Some Plant Derived Compounds In Foods Using Real-Time PCR. The Journal of Food, 42(3), 305-314. https://doi.org/10.15237/gida.GD16110
- Kılıç Altun, S., Yiğin, A., & Demirci, M. (2017). Investigation of The Presence of Listeria spp. in Retailed Yoghurt Samples by Real-Time PCR in Şanlıurfa. Journal of Faculty of Veterinary Medicine, Erciyes University, 14(2), 81-86.
- Kizis, D. (2014). Detection of plant allergens in foods. Food Allergen Testing: Molecular, Immunochemical and Chromatographic Techniques (s. 105-149). In New Jersey: John Wiley & Sons, Ltd. http://dx.doi.org/10.1002/9781118519219
- Kotowicz, M., Adamcyzk, E., & Bania, J. (2007). Application of a Duplex-PCR for detection of Cows'milk in Goats' milk. Annals of Agricultural and Environmental Medicine, 14, 215-218.
- Kubista, M., Andrade, J. M., Bengtsson, M., Forootan, A., Jonak, J., Lind, K., Zoric, N. (2006). The real-time polymerase chain reaction. Molecular Aspects of Medicine, 27(2-3), 95-125. https://doi.org/10.1016/j.mam.2005.12.007
- Liming, S., Zhang, Y., Meng, J., & Bhagwat, A. (2004). Detection of Listeria monocytogenes in Fresh Produce Using Molecular Beacon-Real-time PCR Technology. Journal of Food Science, 69(8), 240-245. https://doi.org/10.1111/j.1750-3841.2004.tb18020.x
- Loparelli, R. M., Cardozzo, B., Balzan, S., Giaccone, V., & Novelli, E. (2007). Real-Time TaqMan Polymerase Chain Reaction Detection and Quantification of Cow DNA in Pure Water Buffalo Mozzarella Cheese: Method Validation and Its Application on Commercial Samples. Journal of Agricultural and Food Chemistry, 55, 3429-3434. https://doi.org/10.1021/jf0637271
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Yıl 2022,
, 53 - 60, 31.12.2022
Erdem Artuvan
,
Salih Aksay
Kaynakça
- Agrimonti, C., Bottari, B., Sardaro, M. L., & Marmiroli, N. (2019). Application of real-time PCR (qPCR) for characterization of microbial populations and type of milk in dairy food products. Critical Reviews in Food Science and Nutrition, 59(3), 423-442. https://doi.org/10.1080/10408398.2017.1375893
- Agrimonti, C., Pirondini, A., Marmiroli, M., & Marmiroli, N. (2015). A quadruplex PCR (qxPCR) assay for adulteration in dairy products. Food Chemistry, 187, 58-64. https://doi.org/10.1016/j.foodchem.2015.04.017
- Ahmed, F. E. (2002). Detection of genetically modified organisms in foods. Trends in Biotechnology, 20(5), 215-223. https://doi.org/10.1016/S0167-7799(01)01920-5
- Alary, R., Serin, A., Maury, D., Jouira, H. B., Sirven, J.-P., Gautier, M.-F., & Joudrier, P. (2002). Comparison of simplex and duplex real-time PCR for the quantification of GMO in maize and soybean. Food Control, 13, 235-244. https://doi.org/10.1016/S0956-7135(02)00015-4
- Ali, M. E., Hashim, U., Dhahi, S. T., Mustafa, S., Man, Y., & Latif, M. A. (2012). Analysis of Pork Adulteration in Commercial Burgers Targeting Porcine-Specific Mitochondrial Cytochrome B Gene by TaqMan Probe Real-Time Polymerase Chain Reaction. Food Analytical Methods, 5, 784-794. http://dx.doi.org/10.1007/s12161-011-9311-4
- Alikord, M., Momtaz, H., Keramat, J., Kadivar, M., & Rad, A. H. (2018). Species identification and animal authentication in meat products: a review. Journal of Food Measurement and Characterization, 12, 145-155. https://doi.org/10.1007/s11694-017-9625-z
- Bottero, M., Civera, T., Nucera, D., Rosati, P., Sacchi, P., & Turi, R. (2003). A multiplex polymerase chain reaction for the identification of cows', goats' and sheep's milk in dairy products. International Dairy Journal, 13, 277-282. http://dx.doi.org/10.1016/S0958-6946(02)00170-X
- Böhme, K., Calo-Mata, P., Barros-Velazquez, J., & Ortea, I. (2019). Review of Recent DNA-Based Methods for Main Food-Authentication Topics. Journal of Agricultural and Food Chemistry, 67, 3854-3864. https://doi.org/10.1021/acs.jafc.8b07016
- Corman, V. M., Landt, O., Kaiser, M., Molenkamp, R., Meijer, A., Chu, D. K., Drosten, C. (2020). Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR. Eurosurveillance, 25(3), 1-8. https://dx.doi.org/10.2807%2F1560-7917.ES.2020.25.3.2000045
- Deniz, H. İ., & Kılıç Altun, S. (2017). The Isolation and Identification of Virulent Listeria monocytogenes Consumption of Local Cheeses in Şanlıurfa Province. Ataturk University Journal of Veterinary Sciences, 12(3), 289-295. http://dx.doi.org/10.17094/ataunivbd.368984
- Di Pinto, A., Terio, V., Marchetti, P., Bottaro, M., Mottola, A., Bozzo, G., Tantillo, G. (2017). DNA-based approach for species identificaton of goat-milk products. Food Chemistry, 229, 93-97. https://doi.org/10.1016/j.foodchem.2017.02.067
- Fagan, J. (2004). DNA Based Methods for Detection and Quantification of GMOs: Principles and Standards. Testing of Genetically Modified Organisms in Foods (s. 163). In New York: Food Products Press.
- Fang, X., & Zhang, C. (2016). Detection of adulterated murine components in meat products by TaqMan real-time PCR. Food Chemistry, 192, 485-490. https://doi.org/10.1016/j.foodchem.2015.07.020
- Ferreira, T., Farah, A., Oliveira, T. C., Lima, I. S., Vitorio, F., & Oliveira, E. M. (2016). Using Real-Time PCR as a tool for monitoring the authenticity of commercial coffees. Food Chemistry, 199, 433-438. https://doi.org/10.1016/j.foodchem.2015.12.045
- Gansbeke, B., Bény, G., De Loose, M., & Taverniers, I. (2018). A TaqMan Real-Time PCR Assay for Apricot (Prunus armeniaca) as an Authenticity Test for Detection of Traces of Persipan in Marzipan. Food Anaytical Methods, 11, 62-68. https://link.springer.com/article/10.1007/s12161-017-0964-5
- Garber, E., Parker, C., Handy, S., Cho, C., Panda, R., Samadpour, M., Ziobro, G. (2016). Presence of Undeclared Food Allergens in Cumin: The Need for Multiplex Methods. Journal of Agricultural and Food Chemistry, 64, 1202-1211. https://doi.org/10.1021/acs.jafc.5b05497
- Gerdes, L., Busch, U., & Pecoraro, S. (2012). Parallelised real-time PCR for identification of maize GMO events. European Food Research and Technology, 234, 315-322. http://dx.doi.org/10.1007%2Fs00217-011-1634-2
- Halkman, K. (2019). Microorganisms in Food. Food Microbiology (p. 309-404). In Ankara: Başak Publishing House.
- Hernandes, M., Ferrando, A., & Rodriguez-Lazaro, D. (2010). Assessment of Genetically Modifi ed Organisms (GMO) in Meat Products by PCR. Handbook of Meat Processing (s. 501-518). In Singapore: Blackwell Publishing. http://dx.doi.org/10.1002/9780813820897.ch29
- Herrero, B., Vieites, J. M., & Espineira, M. (2012). Fast Real-Time PCR for the Detection of Crustacean Allergen in Foods. Journal of Agricultural and Food Chemistry, 60, 1893-1897. https://doi.org/10.1021/jf2043532
- Herrero, B., Vieites, J. M., & Espineira, M. (2014). Development of an in-house fast real-time PCR method for detection of fish allergen in foods and comparison with a commercial kit. Food Chemistry, 151, 415-420. https://doi.org/10.1016/j.foodchem.2013.11.042
- Higuchi, R., Fockler, C., Dollinger, G., & Watson, R. (1993). Kinetic PCR Analysis: Real-time Monitoring of DNA Amplification Reactions. Nature Biotechnology, 11, 1026-1030. https://doi.org/10.1038/nbt0993-1026
- Holland, P. M., Abramson, R. D., Watson, R., & Gelfand, D. H. (1991). Detection of specific polymerase chain reaction product by utilizing the 5'----3' exonuclease activity of Thermus aquaticus DNA polymerase. Proceedings of the National Academy of Sciences of the United States of America, 88(16), 7276-7280. https://dx.doi.org/10.1073%2Fpnas.88.16.7276
- Innis, M. A., & Gelfand, D. H. (1990). Optimization of PCRs. PCR Protocols (s. 3-12). In California: Academic Press, Inc.
- International Service for the Acquisition of Agri-biotech Applications (ISAAA). (2018). Global Status of Commercialized biotech/gm crops: 2018. Accessed From isaaa.org/resources/publications/briefs/54/infographic/pdf/B54-Infographics-English.pdf at 04.11.2020
- Iwobi, A., Sebah, D., Kraemer, I., Losher, C., Fischer, G., Busch, U., & Huber, I. (2015). A multiplex real-time PCR method for the quantification of beef and pork fractions in minced meat. Food Chemistry, 169, 305-313. https://doi.org/10.1016/j.foodchem.2014.07.139
- Iwobi, A., Sebah, D., Spielmann, G., Maggipinto, M., Schrempp, M., Kraemer, I., Huber, I. (2017). A multiplex real-time PCR method for the quantitative determination of equine (horse) fractions in meat products. Food Control, 74, 89-97. https://doi.org/10.1016/j.foodcont.2016.11.035
- JRC European Commission. (2006). Features of Qualitative PCR. Analysis of Genetically Modified Organisms in Food Samples, Course Handbook (s. Section 8). In Italy: European Union Official Publications Office.
- JRC European Commission. (2006). Polymerase Chain Reaction (PCR). Analysis of Genetically Modified Organisms in Food Samples, Course Handbook (s. Section 6). In Italy: European Union Official Publications Office.
- Kabacaoğlu, E., & Karakaş Budak, B. (2019). Detection and quantification of salep with real time PCR utilizing the nr-ITS2 region. Journal of the Science of Food and Agriculture, 99, 2447-2454. https://doi.org/10.1002/jsfa.9453
- Kara, R., & Demirel, Y. N. (2016). Determination of Milk Species Used in Afyon Kaymak Production with RealTime PCR. Ataturk University Journal of Veterinary Sciences, 11(2), 185-190. http://dx.doi.org/10.17094/avbd.77186
- Karakılıç, M., Suna, S., Tamer, C. E., & Çopur, Ö. U. (2014). Food Allergy Reactions and Their Formation Mechanism. Journal of Agricultural Faculty of Uludag University, 28(1), 73-82.
- Kaya, M., Arıkan Asan, A., & Başıaçık Karakoç, Ş. (2019). Determination The Proportion of Chicken and Beef In Meat Products Using Some Commercial Kits. Süleyman Demirel University Journal of Natural and Applied Sciences, 23(1), 52-58. https://doi.org/10.19113/sdufenbed.442199
- Kesmen, Z., Büyükkiraz, M. E., Kahraman, N., & Yetim, H. (2017). Detection of Fraudulent Practices Involving Some Plant Derived Compounds In Foods Using Real-Time PCR. The Journal of Food, 42(3), 305-314. https://doi.org/10.15237/gida.GD16110
- Kılıç Altun, S., Yiğin, A., & Demirci, M. (2017). Investigation of The Presence of Listeria spp. in Retailed Yoghurt Samples by Real-Time PCR in Şanlıurfa. Journal of Faculty of Veterinary Medicine, Erciyes University, 14(2), 81-86.
- Kizis, D. (2014). Detection of plant allergens in foods. Food Allergen Testing: Molecular, Immunochemical and Chromatographic Techniques (s. 105-149). In New Jersey: John Wiley & Sons, Ltd. http://dx.doi.org/10.1002/9781118519219
- Kotowicz, M., Adamcyzk, E., & Bania, J. (2007). Application of a Duplex-PCR for detection of Cows'milk in Goats' milk. Annals of Agricultural and Environmental Medicine, 14, 215-218.
- Kubista, M., Andrade, J. M., Bengtsson, M., Forootan, A., Jonak, J., Lind, K., Zoric, N. (2006). The real-time polymerase chain reaction. Molecular Aspects of Medicine, 27(2-3), 95-125. https://doi.org/10.1016/j.mam.2005.12.007
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