Research Article

Enhancement of indole-3-acetic acid production using Bacillus subtilis-based nano-bioformulations

Number: 35 August 21, 2026

Enhancement of indole-3-acetic acid production using Bacillus subtilis-based nano-bioformulations

Abstract

Indole-3-acetic acid is an important phytohormone synthesized by plant growth-promoting rhizobacteria (PGPR), widely used in agriculture due to its various abilities. This study aimed to develop an economical PGPR-based nano-bioformulation with high Indole-3-acetic acid production capacity. In this context, Bacillus subtilis 4-Ka-22 was used, and initial optimization of the growth medium was carried out. Subsequently, iron salts (FeSO₄ and FeCl₃) were added separately at different concentrations and under varying conditions, both before and after bacterial cultivation. Changes in Indole-3-acetic acid levels were evaluated along with the antagonistic activity and protease enzyme production. In addition, the potential of iron salt addition to induce iron-based nanomaterial/nanoparticle formation was investigated. According to the results, the addition of 15 mM FeSO₄ after cultivation, followed by incubation at 68°C and pH 13 with stirring at 300 rpm for 48 h, led to a 9.15-fold increase in Indole-3-acetic acid production in the B. subtilis 4-Ka-22 culture broth, compared to the control group. These findings suggest that the methodology applied in this study could be adapted to other bacterial strains with similar biosynthetic pathways to enhance Indole-3-acetic acid production. In this regard, the study provides a valuable foundation for the development of PGPR-based nano-biotechnological applications.

Keywords

Supporting Institution

This study is supported by Ege University Scientific Research, Projects Coordination Unit. Project Number: 28513.

Project Number

Project Number: 28513

References

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Details

Primary Language

English

Subjects

Nanobiotechnology, Enzyme and Microbial Biotechnology in Agriculture

Journal Section

Research Article

Publication Date

August 21, 2026

Submission Date

September 30, 2025

Acceptance Date

July 20, 2026

Published in Issue

Year 2026 Number: 35

APA
Filipbe, A. M., Günay, Z., Mis, B., & Eltem, R. (2026). Enhancement of indole-3-acetic acid production using Bacillus subtilis-based nano-bioformulations. Biotech Studies, 35, 2023225. https://doi.org/10.38042/biotechstudies.2023225
AMA
1.Filipbe AM, Günay Z, Mis B, Eltem R. Enhancement of indole-3-acetic acid production using Bacillus subtilis-based nano-bioformulations. Biotech Studies. 2026;(35):2023225. doi:10.38042/biotechstudies.2023225
Chicago
Filipbe, Asiye Meryem, Zülal Günay, Baran Mis, and Rengin Eltem. 2026. “Enhancement of Indole-3-Acetic Acid Production Using Bacillus Subtilis-Based Nano-Bioformulations”. Biotech Studies, nos. 35: 2023225. https://doi.org/10.38042/biotechstudies.2023225.
EndNote
Filipbe AM, Günay Z, Mis B, Eltem R (August 1, 2026) Enhancement of indole-3-acetic acid production using Bacillus subtilis-based nano-bioformulations. Biotech Studies 35 2023225.
IEEE
[1]A. M. Filipbe, Z. Günay, B. Mis, and R. Eltem, “Enhancement of indole-3-acetic acid production using Bacillus subtilis-based nano-bioformulations”, Biotech Studies, no. 35, p. 2023225, Aug. 2026, doi: 10.38042/biotechstudies.2023225.
ISNAD
Filipbe, Asiye Meryem - Günay, Zülal - Mis, Baran - Eltem, Rengin. “Enhancement of Indole-3-Acetic Acid Production Using Bacillus Subtilis-Based Nano-Bioformulations”. Biotech Studies. 35 (August 1, 2026): 2023225. https://doi.org/10.38042/biotechstudies.2023225.
JAMA
1.Filipbe AM, Günay Z, Mis B, Eltem R. Enhancement of indole-3-acetic acid production using Bacillus subtilis-based nano-bioformulations. Biotech Studies. 2026;:2023225.
MLA
Filipbe, Asiye Meryem, et al. “Enhancement of Indole-3-Acetic Acid Production Using Bacillus Subtilis-Based Nano-Bioformulations”. Biotech Studies, no. 35, Aug. 2026, p. 2023225, doi:10.38042/biotechstudies.2023225.
Vancouver
1.Asiye Meryem Filipbe, Zülal Günay, Baran Mis, Rengin Eltem. Enhancement of indole-3-acetic acid production using Bacillus subtilis-based nano-bioformulations. Biotech Studies. 2026 Aug. 1;(35):2023225. doi:10.38042/biotechstudies.2023225


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