Evaluation of anticancerogenic effect of flavonoid rich Verbascum gypsicola Vural & Aydoğdu methanolic extract against SH-SY5Y cell line

Year 2024, Volume: 33 Issue: 1, 1 - 12, 30.06.2024

Seda Şirin

https://doi.org/10.38042/biotechstudies.1383424

Abstract

Neuroblastoma (NB) is an embryonal neoplasm affecting the autonomic branch of the nervous system; it is the most commonly detected cancer type in children. NBs affecting children mostly present with metastatic disease that is hardly treatable with intensive multimodal therapy and portends a poor prognosis. Therefore, the likelihood of children with high-risk NB relapse remains extremely high, which calls for urgent action to discover novel treatment options to improve survival. Assessing the anti-cancer properties of known natural compounds may offer novel therapeutic options against NB. In this study we aimed to investigate the anti-cancer properties of the Verbacum gypsicola methanol extract (VGME) rich in flavonoids on SH-SY5Y cell line. For this purpose, we used LC-MS analysis to investigate the flavonoid composition of VGME, MTT analysis to investigate its effect on cell viability, and flow cytometry and qRT-PCR analyses to investigate its effect on apoptosis. VGME had a high flavonoid content. Its IC50 dose was 50 μg/mL at 48 hours. It significantly increased intracellular ROS level, apoptotic cells’ percentage, and mitochondrial disruption. The capacity of VGME to block cancer growth via an intrinsic apoptotic route implies that it might be a classic option for anticancer drug creation.

Keywords

Apoptosis, Flavonoid, Mitochondrial membrane potential, Reactive oxygen species

Ethical Statement

Not applicable.

Supporting Institution

This research received no specific grant from any funding agency in the public, commercial, or not-for-profit sectors.

Thanks

I would like to thank Prof. Dr. Belma Aslım for providing Verbascum gypsicola Vural & Aydoğdu methanolic extract.

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