Tıbbi Önemi Olan Hyoscyamus niger L.: Fenolik Madde İçeriği ve in vitro Antiproliferatif Aktiviteleri

Abstract

Tıbbi önemi olan bitkiler, zengin bileşenleri nedeniyle kanser de dahil çeşitli hastalıkların tedavisinde en önemli ilaç kaynaklarından birini temsil etmektedirler. Bu çalışmanın amacı, Hyoscyamus niger bitkisinin toprak üstü kısımlarının metanol (HNM) ve su (HNS) ekstraktlarının 17 farklı fenolik madde içeriğini araştırmak, in vitro antiproliferatif aktivitesini değerlendirmektir. Bu amaçla ekstraktların 17 farklı fenolik madde içeriği HPLC ile belirlendi. Antiproliferatif aktivite ise, karaçiğer kanseri hücre hattı (Hep G2), osteosarkoma hücre hattı (U-2 OS) ve sağlıklı fare fibroblast hücre (L-929) hücre hatlarına karşı MTT testi ile tayin edildi. Ekstraktlarda en fazla miktarda askorbik asit belirlendi. HNM ekstraktı içerik yönünden HNS ekstraktından daha zengindi. Antiproliferatif aktivite sonuçlarına göre en güçlü etkiyi HNM ekstraktı, özellikle Hep G2’ye karşı gösterdi. Genel olarak değerlendirildiğinde HNM ekstraktının fenolik madde içeriği ve antiproliferatif aktivite bakımından daha iyi olduğu belirlendi. Bitki ekstraktlarının aktif bileşenlerinin izole edilerek daha fazla in vivo ve in vitro çalışmaların yapılması önem arz etmektedir.

Keywords

• Hyoscyamus niger
• antiproliferatif
• fenolik madde
• in vitro.

Hyoscyamus niger L. of Medical Importance: Phenolic Substance Content and in vitro Antiproliferative Activities

Abstract

Medically important plants represent one of the most important sources of drugs in the treatment of various diseases, including cancer, due to their rich components. The aim of this study was to investigate the content of 17 different phenolic substances of methanol (HNM) and water (HNS) extracts of the aerial parts of the Hyoscyamus niger plant and to evaluate the in vitro antiproliferative activity. For this purpose, the content of 17 different phenolic substances of the extracts was determined by HPLC. Antiproliferative activity was determined by MTT assay against osteosarcoma cell line (U-2 OS), liver cancer cell line (Hep G2) and healthy mouse fibroblast cell (L-929) cell lines. The highest amount of ascorbic acid was determined in the extracts. HNM extract was richer in content than HNS extract. According to the antiproliferative activity results, HNM extract showed the strongest effect, especially against Hep G2. When evaluated in general, it was determined that HNM extract was better in terms of phenolic content and antiproliferative activity. By isolating the active components of plant extracts. It is important to conduct more in vivo and in vitro studies.

Keywords

• Hyoscyamus niger
• Antiproliferative
• Phenolic substance
• in vitro

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