Investigation of Anticholinesterase and Anticancer Effects of Verbascum insulare Boiss. Et Heldr. Extracts Growing in Mus Region

Abstract

İnsanlığın varoluşundan bu yana, bitkiler birçok tedavi yönteminde kullanılmıştır. Scrophulariaceae familyasının bir üyesi olan, Sığırkuyruğu olarakta bilinen Verbascum cinsi üyeleri, çeşitli rahatsızlıklara karşı geleneksel tedavi kullanımlarında değerlendirilmiştir. Bu çalışma ise, Muş yöresinden toplanan bir Verbascum üyesi olan Verbascum insulare Boiss. Et Heldr. 'in biyolojik özelliklerini araştırmaya yöneliktir. V. insulare nin yaprak etanol (L-EtOH), yaprak saf su (L-PW), kök etanol (R-EtOH) ve kök saf su (R-PW) ekstraktları elde edildi. Ekstraktların 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) testi ile HT-29, MCF-7 ve L-929 hücre hatlarına karşı antikanser aktivitesi ve antikolinesteraz aktivitesi incelendi. Etanol ekstraktının kanser hücre hatlarına karşı saf su ekstraktından daha yüksek antikanser aktivite gösterdiği ve daha etkili antikolinerjik etkilere sahip olduğu bulundu. Bu bitkinin antikanser ve antikolinesteraz aktivitesi ile ilgili literatürde daha önce yapılmış bir çalışmaya henüz rastlanılmamıştır. Bu sebeple, çalışmadan elde edilen sonuçlar gelecekte bitkisel ilaç geliştirme çalışmalarına önemli katkılar sunacaktır.

Keywords

• Verbascum insulare Boiss. Et Heldr.
• Antikolinesteraz
• Antikanser

Investigation of Anticholinesterase and Anticancer Effects of Verbascum insulare Boiss. Et Heldr. Extracts Growing in Mus Region

Abstract

Since the existence of humanity, plants have been used in many treatment methods. Members of the genus Verbascum, also known as mullein, a member of the Scrophulariaceae family, have been evaluated for their traditional use against various ailments. In this study, the anticholinesterase and anticancer activities of Verbascum member Verbascum insulare Boiss. Et Heldr. collected from Muş region were investigated. V. insulare leaf ethanol (L-EtOH), leaf purified water (L-PW), root ethanol (R-EtOH), and root purified water (R-PW) extracts were obtained. The anticancer activity of the extracts against HT-29, MCF-7, and L-929 cell lines was examined using the 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test and the anticholinesterase activity was determined. It was found that the ethanol extract showed higher anticancer activity against cancer cell lines than the pure water extract and had more effective anticholinergic influences. No study has been found in the literature on the anticancer and anticholinesterase activity of this plant. Therefore, the results obtained from this study will contribute significantly to the development of herbal medicine in the future.

Keywords

• Verbascum insulare Boiss. Et Heldr.
• Anticholinesterase
• Anticancer

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