Genipin Çapraz Bağlı İnsan Serum Albumin Nanopartikülleri

Abstract

İnsan serum albümin nanoparçacıkların (HSA-NP'ler), kontrollü ilaç salım çalışmalarında ilaç taşıyıcı sistemler olarak kullanımı günümüzde önem kazanmıştır. Albümin nanoparçacıkları, biyolojik olarak uyumludur, biyolojik olarak parçalanabilir ve sürekli salım sağlar. HSA nanoparçacıkların uzun süreli ilaç taşıyabilmesi için çapraz bağlanması gerekmektedir ve literatürde genellikle hücreler üzerinde toksik etkisi olan glutaraldehit ile çapraz baglama gerçekleştirilmektedir. Bu çalışmada, HSA nanoparçacıklarının desolvasyon tekniği ile başarılı bir şekilde üretilmesi için biyolojik bir kovalent çapraz bağlayıcı olan genipin kullanılmıştır. Çapraz bağlama süresini azaltmak için iki farklı sıcaklık ve genipin konsantrasyonu çalışılmıştır. Üretilen HSA nanoparçacıklarının karakterizasyonu, Taramalı Elektron Mikroskobisi (SEM) ve Dinamik Işık Saçılımı (DLS) yöntemleri ile gerçekleştirilmiştir. Sıcaklık oda sıcaklığından 37oC'ye yükseltilerek çapraz bağlanma süresi 8-24 saatten 2 saate düşürülmüştür. Genipin ile çapraz bağlanan HSA nanoparçacıkların, ilaç taşıyıcı sistemlerde ve kişiselleştirilmiş tıp uygulamalarında kullanımı potansiyel taşımaktadır.

Keywords

• HSA
• Genipin
• Nanoparçacık
• Desolvasyon

Project Number

20332001

Genipin Crosslinked Human Serum Albumin Nanoparticles

Abstract

The use of human serum albumin nanoparticles (HSA-NPs) as drug delivery systems in controlled drug release studies has gained importance today. Albumin nanoparticles are biocompatible, biodegradable and provide sustained release. To maintain long-term drug delivery, HSA nanoparticles need to be cross-linked. A chemical crosslinker, glutaraldehyde is generally used in the literature and has some toxic effects on the cells. In this study, a biological crosslinker, genipin, was used for the production of HSA nanoparticles by desolvation technique. Two different temperatures and genipin concentrations were studied in order to decrease crosslinking time. The nanoparticles were characterized by Scanning Electron Microscopy (SEM) and Dynamic Light Scattering (DLS). The crosslinking time was reduced from 8-24 hours to 2 hours by raising the temperature to 37oC from room temperature. HSA nanoparticles which are crosslinked by genipin may have potential use in drug delivery system and may be applied in personalized medicine applications.

Keywords

• HSA
• Genipin
• Nanoparticle
• Desolvation

Supporting Institution

Scientific Research Projects Coordination Unit of Adana Alparslan Türkeş Science and Technology University

Project Number

20332001

Thanks

This study was supported by the Scientific Research Projects Coordination Unit of Adana Alparslan Türkeş Science and Technology University (BAP) with the project number 20332001. Dila Kurtul was supported by the TÜBİTAK-BİDEB 2210-C National Scholarship in Priority Fields in Science Program for MSc students.

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