Determination of Enzyme Inhibitory and Antioxidant Activities of Selected Oak Galls

Nesrin Haşimi; Merve Doğan Abdioğlu; Mehmet Boğa

doi:10.17798/bitlisfen.1669087

Determination of Enzyme Inhibitory and Antioxidant Activities of Selected Oak Galls

Abstract

In this study, the enzyme inhibition and antioxidant activities of methanol extracts obtained from galls induced by Andricus quercustozae and Andricus cecconii on Quercus brantii Lindley. and Andricus quercusramuli on Quercus infectoria Oliver were investigated. The enzyme inhibition activity was assessed using spectrophotometric methods for acetylcholinesterase (AChE), butyrylcholinesterase (BChE), tyrosinase, and urease inhibition. The total phenolic and flavonoid contents of the extracts were quantified as gallic acid and quercetin equivalents, respectively. Antioxidant activity was evaluated using three distinct assays: DPPH free radical scavenging, ABTS cation radical scavenging, and CUPRAC. Regarding cholinesterase inhibition, while none of the extracts exhibited activity against AChE, they demonstrated inhibition of BChE, with A. quercustozae gall extract showing the highest activity (31.33 ± 1.25%). Similarly, in tyrosinase inhibition, A. quercustozae gall extract exhibited the most significant effect, with an inhibition rate of 61.16 ± 0.72%. In urease inhibition, A. quercustozae gall extract was the only sample to demonstrate activity (40.98 ± 1.41%), whereas A. quercusramuli and A. cecconii extracts showed no significant effect. The total phenolic contents of the extracts were higher than their total flavonoid contents, correlating with their strong antioxidant activities. Some extracts exhibited higher antioxidant activity than standard positive controls, further emphasizing their potential as natural antioxidant sources.

Keywords

Supporting Institution

Batman University

Project Number

.18.001.

Ethical Statement

Ethics committee approval is not required for this study

Thanks

This study is Merve Doğan Abdioğlu's master's thesis and supported by Batman University Scientific Research Committee with project number .18.001. We thank Dr. Erdem Seven for his assistance in collecting and identifying the samples.

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Details

Primary Language

English

Subjects

Analytical Biochemistry, Enzymes

Journal Section

Research Article

Authors

Nesrin Haşimi ^*
0000-0003-1367-5624
Türkiye

Merve Doğan Abdioğlu
0009-0006-2691-5016
Türkiye

Mehmet Boğa
0000-0003-4163-9962
Türkiye

Publication Date

September 30, 2025

Submission Date

April 2, 2025

Acceptance Date

September 23, 2025

Published in Issue

Year 2025 Volume: 14 Number: 3

DOI

https://doi.org/10.17798/bitlisfen.1669087

IZ

https://izlik.org/JA79SA75NF

Cite

RIS / Bibtex

APA

Haşimi, N., Doğan Abdioğlu, M., & Boğa, M. (2025). Determination of Enzyme Inhibitory and Antioxidant Activities of Selected Oak Galls. Bitlis Eren Üniversitesi Fen Bilimleri Dergisi, 14(3), 1487-1503. https://doi.org/10.17798/bitlisfen.1669087

AMA

1.Haşimi N, Doğan Abdioğlu M, Boğa M. Determination of Enzyme Inhibitory and Antioxidant Activities of Selected Oak Galls. Bitlis Eren Üniversitesi Fen Bilimleri Dergisi. 2025;14(3):1487-1503. doi:10.17798/bitlisfen.1669087

Chicago

Haşimi, Nesrin, Merve Doğan Abdioğlu, and Mehmet Boğa. 2025. “Determination of Enzyme Inhibitory and Antioxidant Activities of Selected Oak Galls”. Bitlis Eren Üniversitesi Fen Bilimleri Dergisi 14 (3): 1487-1503. https://doi.org/10.17798/bitlisfen.1669087.

EndNote

Haşimi N, Doğan Abdioğlu M, Boğa M (September 1, 2025) Determination of Enzyme Inhibitory and Antioxidant Activities of Selected Oak Galls. Bitlis Eren Üniversitesi Fen Bilimleri Dergisi 14 3 1487–1503.

IEEE

[1]N. Haşimi, M. Doğan Abdioğlu, and M. Boğa, “Determination of Enzyme Inhibitory and Antioxidant Activities of Selected Oak Galls”, Bitlis Eren Üniversitesi Fen Bilimleri Dergisi, vol. 14, no. 3, pp. 1487–1503, Sept. 2025, doi: 10.17798/bitlisfen.1669087.

ISNAD

Haşimi, Nesrin - Doğan Abdioğlu, Merve - Boğa, Mehmet. “Determination of Enzyme Inhibitory and Antioxidant Activities of Selected Oak Galls”. Bitlis Eren Üniversitesi Fen Bilimleri Dergisi 14/3 (September 1, 2025): 1487-1503. https://doi.org/10.17798/bitlisfen.1669087.

JAMA

1.Haşimi N, Doğan Abdioğlu M, Boğa M. Determination of Enzyme Inhibitory and Antioxidant Activities of Selected Oak Galls. Bitlis Eren Üniversitesi Fen Bilimleri Dergisi. 2025;14:1487–1503.

MLA

Haşimi, Nesrin, et al. “Determination of Enzyme Inhibitory and Antioxidant Activities of Selected Oak Galls”. Bitlis Eren Üniversitesi Fen Bilimleri Dergisi, vol. 14, no. 3, Sept. 2025, pp. 1487-03, doi:10.17798/bitlisfen.1669087.

Vancouver

1.Nesrin Haşimi, Merve Doğan Abdioğlu, Mehmet Boğa. Determination of Enzyme Inhibitory and Antioxidant Activities of Selected Oak Galls. Bitlis Eren Üniversitesi Fen Bilimleri Dergisi. 2025 Sep. 1;14(3):1487-503. doi:10.17798/bitlisfen.1669087