Production and Characterization of Bacterial Cellulose from Traditional Homemade Vinegar: Overview of Biotechnological Applications

Year 2025, Volume: 14 Issue: 4, 2374 - 2385, 31.12.2025

Abdulkadir Akkurt , Aysun Ergene , Belgin Güldeste

https://doi.org/10.17798/bitlisfen.1737056

https://izlik.org/JA97HJ55UR

Abstract

Cellulose is a polymer that can be produced not only by plants but also by some bacteria. The gelatinous layer known as "mother of vinegar" is an example of bacterial cellulose (BC), which is formed on the surface during the process of traditional homemade vinegar fermentation.
In this study, bacterial cellulose isolated from homemade vinegar produced by the traditional method was produced, and the resulting polymer was characterized by Fourier transform infrared (FTIR) spectroscopy and scanning electron microscopy (SEM).
FTIR analysis demonstrated that the chemical bonding of the obtained bacterial cellulose is characteristic of cellulose. The presence of typical peaks, including a broad -OH stretching band (~3340 cm-1) and an aliphatic C-H stretching band (~2890 cm-1), was observed. Scanning electron microscopy (SEM) images revealed that the bacterial cellulose exhibited a nanofibrous network structure with elevated porosity. The characterization results show that bacterial cellulose exhibits high water retention capacity and mechanical strength due to its pure, fibrous nanostructure. It is clear that bacterial cellulose has many advantages. Specifically, its potential applications include the fields of biomedical research, namely in the domains of wound dressings, tissue scaffolds and controlled drug release. Additionally, its relevance extends to food science, which can be employed as a low-calorie food ingredient, a texturing agent or a coating material. In conclusion, it has been demonstrated that bacterial cellulose derived from traditional fermentation products, such as homemade vinegar, can be utilized as a high-value-added biomaterial.
With these superior properties, bacterial cellulose has potential applications in biomedical fields (particularly wound dressings, tissue scaffolds and controlled drug delivery) and food applications (low-calorie food ingredient, texture and coating material).

Keywords

Bacterial cellulose , characterization , FTIR; , SEM , biomaterials

Ethical Statement

The study is complied with research and publication ethics.

Thanks

This article is derived from the doctoral dissertation titled 'Production and Characterization of Bacterial Cellulose from Traditional Homemade Vinegar: Overview of Biotechnological Applications', completed by Abdulkadir Akkurt at Kırıkkale University, Graduate School of Natural Sciences in 2025.

References

• N. Chandel, K. Jain, A. Jain, T. Raj, A. K. Patel, Y. H. Yang, & S. K. Bhatia, “The versatile world of cellulose-based materials in healthcare: from production to applications.” Industrial Crops and Products, vol. 201, no. 116929, 2023.
• S. Dutta, J. Kim, Y. Ide, J. H. Kim, M. S. A. Hossain, Y. Bando, K. C. W. Wu, “3D network of cellulose-based energy storage devices and related emerging applications,” Materials Horizons, vol. 4, no. 4, pp. 522-545, 2017.
• Y. Huang, C. Zhu, J. Yang, Y. Nie, C. Chen, and D. Sun, “Recent advances in bacterial cellulose,” Cellulose, vol. 21, no. 1, pp. 1–30, 2014.
• Mehrotra R, Sharma S, Shree N, Kaur K, Ed., “Bacterial Cellulose: An Ecological Alternative as a Biotextile”, Biosci Biotech Res Asia, vol. 20, no. 2, 2023.
• J. Ahmed, M. Gultekinoglu, and M. Edirisinghe, “Bacterial cellulose micro-nano fibres for wound healing applications,” Biotechnol. Adv., vol. 41, no. 107549, p. 107549, 2020.
• Y. Z. Wan, H. Luo, F. He, H. Liang, Y. Huang, and X. L. Li, “Mechanical, moisture absorption, and biodegradation behaviours of bacterial cellulose fibre-reinforced starch biocomposites,” Compos. Sci. Technol., vol. 69, no. 7–8, pp. 1212–1217, 2009.
• A. J. Brown, “XLIII. On an acetic ferment which forms cellulose,” J. Chem. Soc., vol. 49, no. 0, pp. 432–439, 1886.
• P. Shrivastav, S. Pramanik, G. Vaidya, M. A. Abdelgawad, M. M. Ghoneim, A. Singh, & M. A. Abourehab, “Bacterial cellulose as a potential biopolymer in biomedical applications: A state-of-the-art review,” Journal of materials chemistry B., vol. 10, no. 17, pp. 3199-3241, 2022.
• N.-N. Nguyen, T. T. V. Tran, Q.-D. Nguyen, T.-P. Nguyen, and T.-N. Lien, “Modification of microstructure and selected physicochemical properties of bacterial cellulose produced by bacterial isolate using hydrocolloid-fortified Hestrin-Schramm medium,” Biotechnol. Prog., vol. 39, no. 4, p. e3344, 2023.
• K. H. Al-Ramlawee and E. J. Alkalifawi, “Production and characterization of bacterial cellulose utilizing Iraqi vinegar’s mother pellicles,” Journal of Applied & Natural Science, vol. 15, no. 4, 2023.
• M. Kacuráková, A. C. Smith, M. J. Gidley, and R. H. Wilson, “Molecular interactions in bacterial cellulose composites studied by 1D FT-IR and dynamic 2D FT-IR spectroscopy,” Carbohydr. Res., vol. 337, no. 12, pp. 1145–1153, 2002.
• M. Iguchi, S. Yamanaka, and A. Budhiono, “Bacterial cellulose—a masterpiece of nature’s arts,” J. Mater. Sci., vol. 35, no. 2, pp. 261–270, 2000.
• M. Güzel and Ö. Akpınar, “Production and characterization of bacterial cellulose from citrus peels,” Waste Biomass Valorization, vol. 10, no. 8, pp. 2165–2175, 2019.
• A. Akoğlu, A. G. Karahan, M. L. Çakmakçı, and İ. Çakır, “Bakteriyel Selülozun Özellikleri ve Gıda Sanayisinde Kullanımı,” Gıda, vol. 35, no. 2, pp. 127–134, 2010.
• M. Ul-Islam, T. Khan, and J. K. Park, “Water holding and release properties of bacterial cellulose obtained by in situ and ex situ modification,” Carbohydr. Polym., vol. 88, no. 2, pp. 596–603, 2012.
• N. A. Arkharova, E. I. Suvorova, A. V. Severin, A. K. Khripunov, S. V. Krasheninnikov, and V. V. Klechkovskaya, “SEM and TEM for structure and properties characterization of bacterial cellulose/hydroxyapatite composites,” Scanning, vol. 38, no. 6, pp. 757–765, 2016.
• H. M. C. Azeredo, H. Barud, C. S. Farinas, V. M. Vasconcellos, and A. M. Claro, “Bacterial cellulose as a raw material for food and food packaging applications,” Front. Sustain. Food Syst., vol. 3, 2019.
• C. J. S. Galdino Jr, A. D. Maia, H. M. Meira, T. C. Souza, J. D. Amorim, F. C. Almeida, & L. A. Sarubbo, “ Use of a bacterial cellulose filter for the removal of oil from wastewater,” Process Biochemistry, vol. 91, pp. 288-296, 2020.
• M. L. Cacicedo, M. C. Castro, I. Servetas, L. Bosnea, K. Boura, P. Tsafrakidou, & G. R. Castro, “Progress in bacterial cellulose matrices for biotechnological applications,” Bioresource technology, vol. 213, pp. 172-180, 2016.
• M. Güzel, “Characterization of cellulose produced by bacteria isolated from different vinegars,” Int. J. Biol. Macromol., vol. 277, no. Pt 3, p. 134436, 2024.
• K. H. AL-Ramlawee and E. J. Alkalifawi, “Production and characterization of bacterial cellulose utilizing Iraqi vinegar’s mother pellicles,” J. Appl. Nat. Sci., vol. 15, no. 4, pp. 1619–1626, 2023.
• P. Jacek, F. Dourado, M. Gama, and S. Bielecki, “Molecular aspects of bacterial nanocellulose biosynthesis,” Microb. Biotechnol., vol. 12, no. 4, pp. 633–649, 2019.