Competitive Inhibition of Glutathione Reductase Activity by Cyclohexyl-(4,5-dihydro-1H-imidazol-2-yl)amine hydroiodide and Benzyl-(4,5-dihydro-1H-imidazol-2-yl)amine hydroiodide

Year 2025, Volume: 14 Issue: 4, 2386 - 2394, 31.12.2025

Hasan Karadağ , Murat Genç

https://doi.org/10.17798/bitlisfen.1740818

https://izlik.org/JA59WH44BP

Abstract

In this research, 0, 25, 50, 100, 200 and 400 mg/L (ppm) concentrations of 2-aminoimidazolines (cyclohexyl-(4,5-dihydro-1H-imidazol-2-yl)amine hydroiodide (CHX) and benzyl-(4,5-dihydro-1H-imidazol-2-yl)amine hydroiodide (BNZ)) were applied to baker's yeast (Saccharomyces cerevisiae) glutathione reductase (GR). Since the inhibition constant (Ki) (43 ± 2 nM) and 50 % inhibition concentration (IC50) (947 mg/L =3.13×10-3 M) values of BNZ were found to be smaller than the Ki (59 ± 17 nM) and IC50 (1461 mg/L =4.95×10-3 M) values of CHX. At imidazoline concentrations of 400 mg/L, CHX reduced GR activity by 16.07 %, while BNZ reduced GR activity by 21.96 %. BNZ inhibited GR more than CHX. In addition, molecular docking results also support the experimental data. CHX and BNZ inhibited GR enzyme activity as competitively.

Keywords

Benzyl-(4 , 5-dihydro-1H-imidazol-2-yl)amine hydroiodide , Cyclohexyl-(4 , Enzymes , Inhibitors , Imidazoline.

Ethical Statement

The study is complied with research and publication ethics

Supporting Institution

No Supporting Institution

Project Number

No funding

Thanks

The authors thank to the Departments of Chemistry and Biology, Faculty of Science and Letters, Adıyaman University, for providing the opportunity to use their laboratories and thank ADYUMLAB for measurement of 2-aminoimidazolines concentrations.

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