Competitive Inhibition of Glutathione Reductase Activity by Cyclohexyl-(4,5-dihydro-1H-imidazol-2-yl)amine hydroiodide and Benzyl-(4,5-dihydro-1H-imidazol-2-yl)amine hydroiodide

Hasan Karadağ; Murat Genç

doi:10.17798/bitlisfen.1740818

Competitive Inhibition of Glutathione Reductase Activity by Cyclohexyl-(4,5-dihydro-1H-imidazol-2-yl)amine hydroiodide and Benzyl-(4,5-dihydro-1H-imidazol-2-yl)amine hydroiodide

Abstract

In this research, 0, 25, 50, 100, 200 and 400 mg/L (ppm) concentrations of 2-aminoimidazolines (cyclohexyl-(4,5-dihydro-1H-imidazol-2-yl)amine hydroiodide (CHX) and benzyl-(4,5-dihydro-1H-imidazol-2-yl)amine hydroiodide (BNZ)) were applied to baker's yeast (Saccharomyces cerevisiae) glutathione reductase (GR). Since the inhibition constant (Ki) (43 ± 2 nM) and 50 % inhibition concentration (IC50) (947 mg/L =3.13×10-3 M) values of BNZ were found to be smaller than the Ki (59 ± 17 nM) and IC50 (1461 mg/L =4.95×10-3 M) values of CHX. At imidazoline concentrations of 400 mg/L, CHX reduced GR activity by 16.07 %, while BNZ reduced GR activity by 21.96 %. BNZ inhibited GR more than CHX. In addition, molecular docking results also support the experimental data. CHX and BNZ inhibited GR enzyme activity as competitively.

Keywords

Supporting Institution

No Supporting Institution

Project Number

No funding

Ethical Statement

The study is complied with research and publication ethics

Thanks

The authors thank to the Departments of Chemistry and Biology, Faculty of Science and Letters, Adıyaman University, for providing the opportunity to use their laboratories and thank ADYUMLAB for measurement of 2-aminoimidazolines concentrations.

References

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Details

Primary Language

English

Subjects

Enzymes

Journal Section

Research Article

Authors

Hasan Karadağ ^*
0000-0001-5701-8445
Türkiye

Murat Genç
0000-0003-1224-4128
Türkiye

Publication Date

December 31, 2025

Submission Date

July 12, 2025

Acceptance Date

October 17, 2025

Published in Issue

Year 2025 Volume: 14 Number: 4

DOI

https://doi.org/10.17798/bitlisfen.1740818

IZ

https://izlik.org/JA59WH44BP

Cite

RIS / Bibtex

APA

Karadağ, H., & Genç, M. (2025). Competitive Inhibition of Glutathione Reductase Activity by Cyclohexyl-(4,5-dihydro-1H-imidazol-2-yl)amine hydroiodide and Benzyl-(4,5-dihydro-1H-imidazol-2-yl)amine hydroiodide. Bitlis Eren Üniversitesi Fen Bilimleri Dergisi, 14(4), 2386-2394. https://doi.org/10.17798/bitlisfen.1740818

AMA

1.Karadağ H, Genç M. Competitive Inhibition of Glutathione Reductase Activity by Cyclohexyl-(4,5-dihydro-1H-imidazol-2-yl)amine hydroiodide and Benzyl-(4,5-dihydro-1H-imidazol-2-yl)amine hydroiodide. Bitlis Eren Üniversitesi Fen Bilimleri Dergisi. 2025;14(4):2386-2394. doi:10.17798/bitlisfen.1740818

Chicago

Karadağ, Hasan, and Murat Genç. 2025. “Competitive Inhibition of Glutathione Reductase Activity by Cyclohexyl-(4,5-Dihydro-1H-Imidazol-2-Yl)amine Hydroiodide and Benzyl-(4,5-Dihydro-1H-Imidazol-2-Yl)amine Hydroiodide”. Bitlis Eren Üniversitesi Fen Bilimleri Dergisi 14 (4): 2386-94. https://doi.org/10.17798/bitlisfen.1740818.

EndNote

Karadağ H, Genç M (December 1, 2025) Competitive Inhibition of Glutathione Reductase Activity by Cyclohexyl-(4,5-dihydro-1H-imidazol-2-yl)amine hydroiodide and Benzyl-(4,5-dihydro-1H-imidazol-2-yl)amine hydroiodide. Bitlis Eren Üniversitesi Fen Bilimleri Dergisi 14 4 2386–2394.

IEEE

[1]H. Karadağ and M. Genç, “Competitive Inhibition of Glutathione Reductase Activity by Cyclohexyl-(4,5-dihydro-1H-imidazol-2-yl)amine hydroiodide and Benzyl-(4,5-dihydro-1H-imidazol-2-yl)amine hydroiodide”, Bitlis Eren Üniversitesi Fen Bilimleri Dergisi, vol. 14, no. 4, pp. 2386–2394, Dec. 2025, doi: 10.17798/bitlisfen.1740818.

ISNAD

Karadağ, Hasan - Genç, Murat. “Competitive Inhibition of Glutathione Reductase Activity by Cyclohexyl-(4,5-Dihydro-1H-Imidazol-2-Yl)amine Hydroiodide and Benzyl-(4,5-Dihydro-1H-Imidazol-2-Yl)amine Hydroiodide”. Bitlis Eren Üniversitesi Fen Bilimleri Dergisi 14/4 (December 1, 2025): 2386-2394. https://doi.org/10.17798/bitlisfen.1740818.

JAMA

1.Karadağ H, Genç M. Competitive Inhibition of Glutathione Reductase Activity by Cyclohexyl-(4,5-dihydro-1H-imidazol-2-yl)amine hydroiodide and Benzyl-(4,5-dihydro-1H-imidazol-2-yl)amine hydroiodide. Bitlis Eren Üniversitesi Fen Bilimleri Dergisi. 2025;14:2386–2394.

MLA

Karadağ, Hasan, and Murat Genç. “Competitive Inhibition of Glutathione Reductase Activity by Cyclohexyl-(4,5-Dihydro-1H-Imidazol-2-Yl)amine Hydroiodide and Benzyl-(4,5-Dihydro-1H-Imidazol-2-Yl)amine Hydroiodide”. Bitlis Eren Üniversitesi Fen Bilimleri Dergisi, vol. 14, no. 4, Dec. 2025, pp. 2386-94, doi:10.17798/bitlisfen.1740818.

Vancouver

1.Hasan Karadağ, Murat Genç. Competitive Inhibition of Glutathione Reductase Activity by Cyclohexyl-(4,5-dihydro-1H-imidazol-2-yl)amine hydroiodide and Benzyl-(4,5-dihydro-1H-imidazol-2-yl)amine hydroiodide. Bitlis Eren Üniversitesi Fen Bilimleri Dergisi. 2025 Dec. 1;14(4):2386-94. doi:10.17798/bitlisfen.1740818