Total Phenolic Content, in vitro Antibacterial, Antioxidant, and Anticancer Activities of the Ethanol Extract of Rosehip (Rosa canina L.) Naturally Distributed in Elazığ Province

İlhan Sabancılar; İbrahim Koç; Erdal Öğün; Seçkin Kaya; Velid Unsal

doi:10.17798/bitlisfen.1750416

Total Phenolic Content, in vitro Antibacterial, Antioxidant, and Anticancer Activities of the Ethanol Extract of Rosehip (Rosa canina L.) Naturally Distributed in Elazığ Province

Abstract

Research continues on the identification of plants with activities such as antibacterial, antioxidant, and anticancer properties. This study aimed to determine the total phenolic content of the ethanol extract of rosehip (Rosa canina L.) fruits grown in Elazığ, Türkiye, as well as its in vitro antibacterial, antioxidant, and anticancer activities. Antibacterial activity was assessed using the Agar Well Diffusion Method; antioxidant activities were evaluated using the DPPH and CUPRAC methods; and anticancer studies were conducted on A549 (ATCC CCL-185), U2OS (ATCC HTB-96), and HUVEC (ATCC CRL-1730) cell lines using the MTT assay. Optimization studies were performed according to Response Surface Methodology. As a result, it was found that the total phenolic content of the extract was 79.013±3.5; it was determined that aqueous alcohol extracts had antimicrobial effects only on B. cereus and did not have any antimicrobial effects on E. coli, P. aeruginosa and Stp. aureus bacteria, and it demonstrated a dose- and time-dependent cytotoxic effect on different cell lines. The most pronounced effect was observed in the HUVEC cell line, while the effects increased with prolonged incubation time in the A549 and U2OS cell lines.

Keywords

Ethical Statement

The study is complied with research and publication ethics.

Thanks

The authors would like to thank Prof. Dr. İnanç Özgen, who collected the plant material of Rosa canina L. (rosehip) from Sütlüce Village, Elazığ Province, on October 20, 2024. The specimen was identified by Prof. Dr. Murat Kürşat and deposited in the Herbarium of Bitlis Eren University (BEU) under the voucher number BEU1111. Their valuable contributions to this study are greatly appreciated.

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Details

Primary Language

English

Subjects

Cell Development, Proliferation and Death

Journal Section

Research Article

Authors

İlhan Sabancılar ^*
0000-0002-0773-2752
Türkiye

İbrahim Koç
0000-0003-0803-6801
Türkiye

Erdal Öğün
0000-0001-8280-4245
Türkiye

Seçkin Kaya
0000-0001-8771-0109
Türkiye

Velid Unsal
0000-0003-1415-0563
Türkiye

Publication Date

December 31, 2025

Submission Date

July 25, 2025

Acceptance Date

October 28, 2025

Published in Issue

Year 2025 Volume: 14 Number: 4

DOI

https://doi.org/10.17798/bitlisfen.1750416

IZ

https://izlik.org/JA48CH72SY

Cite

RIS / Bibtex

APA

Sabancılar, İ., Koç, İ., Öğün, E., Kaya, S., & Unsal, V. (2025). Total Phenolic Content, in vitro Antibacterial, Antioxidant, and Anticancer Activities of the Ethanol Extract of Rosehip (Rosa canina L.) Naturally Distributed in Elazığ Province. Bitlis Eren Üniversitesi Fen Bilimleri Dergisi, 14(4), 2488-2508. https://doi.org/10.17798/bitlisfen.1750416

AMA

1.Sabancılar İ, Koç İ, Öğün E, Kaya S, Unsal V. Total Phenolic Content, in vitro Antibacterial, Antioxidant, and Anticancer Activities of the Ethanol Extract of Rosehip (Rosa canina L.) Naturally Distributed in Elazığ Province. Bitlis Eren Üniversitesi Fen Bilimleri Dergisi. 2025;14(4):2488-2508. doi:10.17798/bitlisfen.1750416

Chicago

Sabancılar, İlhan, İbrahim Koç, Erdal Öğün, Seçkin Kaya, and Velid Unsal. 2025. “Total Phenolic Content, in Vitro Antibacterial, Antioxidant, and Anticancer Activities of the Ethanol Extract of Rosehip (Rosa Canina L.) Naturally Distributed in Elazığ Province”. Bitlis Eren Üniversitesi Fen Bilimleri Dergisi 14 (4): 2488-2508. https://doi.org/10.17798/bitlisfen.1750416.

EndNote

Sabancılar İ, Koç İ, Öğün E, Kaya S, Unsal V (December 1, 2025) Total Phenolic Content, in vitro Antibacterial, Antioxidant, and Anticancer Activities of the Ethanol Extract of Rosehip (Rosa canina L.) Naturally Distributed in Elazığ Province. Bitlis Eren Üniversitesi Fen Bilimleri Dergisi 14 4 2488–2508.

IEEE

[1]İ. Sabancılar, İ. Koç, E. Öğün, S. Kaya, and V. Unsal, “Total Phenolic Content, in vitro Antibacterial, Antioxidant, and Anticancer Activities of the Ethanol Extract of Rosehip (Rosa canina L.) Naturally Distributed in Elazığ Province”, Bitlis Eren Üniversitesi Fen Bilimleri Dergisi, vol. 14, no. 4, pp. 2488–2508, Dec. 2025, doi: 10.17798/bitlisfen.1750416.

ISNAD

Sabancılar, İlhan - Koç, İbrahim - Öğün, Erdal - Kaya, Seçkin - Unsal, Velid. “Total Phenolic Content, in Vitro Antibacterial, Antioxidant, and Anticancer Activities of the Ethanol Extract of Rosehip (Rosa Canina L.) Naturally Distributed in Elazığ Province”. Bitlis Eren Üniversitesi Fen Bilimleri Dergisi 14/4 (December 1, 2025): 2488-2508. https://doi.org/10.17798/bitlisfen.1750416.

JAMA

1.Sabancılar İ, Koç İ, Öğün E, Kaya S, Unsal V. Total Phenolic Content, in vitro Antibacterial, Antioxidant, and Anticancer Activities of the Ethanol Extract of Rosehip (Rosa canina L.) Naturally Distributed in Elazığ Province. Bitlis Eren Üniversitesi Fen Bilimleri Dergisi. 2025;14:2488–2508.

MLA

Sabancılar, İlhan, et al. “Total Phenolic Content, in Vitro Antibacterial, Antioxidant, and Anticancer Activities of the Ethanol Extract of Rosehip (Rosa Canina L.) Naturally Distributed in Elazığ Province”. Bitlis Eren Üniversitesi Fen Bilimleri Dergisi, vol. 14, no. 4, Dec. 2025, pp. 2488-0, doi:10.17798/bitlisfen.1750416.

Vancouver

1.İlhan Sabancılar, İbrahim Koç, Erdal Öğün, Seçkin Kaya, Velid Unsal. Total Phenolic Content, in vitro Antibacterial, Antioxidant, and Anticancer Activities of the Ethanol Extract of Rosehip (Rosa canina L.) Naturally Distributed in Elazığ Province. Bitlis Eren Üniversitesi Fen Bilimleri Dergisi. 2025 Dec. 1;14(4):2488-50. doi:10.17798/bitlisfen.1750416