Determination of Phenolic Content and Antioxidant Activities of Moringa oleifera Extracts

Bihter Şahin; Gülsüm Aktaş

doi:10.17798/bitlisfen.1866992

Determination of Phenolic Content and Antioxidant Activities of Moringa oleifera Extracts

Abstract

Moringa oleifera leaves, seeds, pods, flowers, and roots have been utilized in traditional medicine and various cultural practices for centuries. In this study five different Moringa oleifera leaf extracts hexane, ethyl acetate, ethanol, methanol, and water were obtained from plant material collected in Muğla, Türkiye, in 2022. Except for the hexane extract, all samples were analyzed for their phenolic profile using HPLC-DAD. This analysis allowed for both qualitative and quantitative identification of phenolic compounds. The antioxidant activities of the extracts were evaluated by determining their IC50 values using three different in vitro assays. Based on the data obtained, the extracts demonstrated moderate antioxidant activity compared to standard reference antioxidants BHA and α-tocopherol, which served as controls with known polarities. Among the identified phenolic compounds, rutin was found to be a common constituent in all extracts. Notably, the methanol (MO4) and water (MO5) extracts exhibited a richer phenolic profile, as they contained a higher number of phenolic compounds based on the screening of 41 phenolic standards employed in the HPLC-DAD method. These findings indicate that the variation in extract polarity significantly influences both the phenolic composition and antioxidant activity of Moringa oleifera leaves. Overall, the results suggest that Moringa oleifera may serve as a potential source of natural antioxidants, with methanol and water being the most effective solvents for extracting bioactive phenolic profile.

Keywords

Supporting Institution

TUBITAK

Project Number

1919B012221744

Ethical Statement

The authors declare that this study complies with Research and Publication Ethics.

Thanks

I would like to thank Prof. Dr. Mehmet Öztürk, who provided the opportunity to use laboratory facilities, Assoc. Prof. Dr. Yusuf Sıcak, who supported me in finding the information and material.

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Details

Primary Language

English

Subjects

Natural Products and Bioactive Compounds

Journal Section

Research Article

Authors

Bihter Şahin ^*
0000-0001-8657-052X
Türkiye

Gülsüm Aktaş
0009-0007-3218-4974
Türkiye

Publication Date

September 30, 2026

Submission Date

January 19, 2026

Acceptance Date

April 22, 2026

Published in Issue

Year 2026 Volume: 15 Number: 3

DOI

https://doi.org/10.17798/bitlisfen.1866992

IZ

https://izlik.org/JA97PJ42BZ

Cite

RIS / Bibtex

APA

Şahin, B., & Aktaş, G. (2026). Determination of Phenolic Content and Antioxidant Activities of Moringa oleifera Extracts. Bitlis Eren Üniversitesi Fen Bilimleri Dergisi, 15(3), 1138-1146. https://doi.org/10.17798/bitlisfen.1866992

AMA

1.Şahin B, Aktaş G. Determination of Phenolic Content and Antioxidant Activities of Moringa oleifera Extracts. Bitlis Eren Üniversitesi Fen Bilimleri Dergisi. 2026;15(3):1138-1146. doi:10.17798/bitlisfen.1866992

Chicago

Şahin, Bihter, and Gülsüm Aktaş. 2026. “Determination of Phenolic Content and Antioxidant Activities of Moringa Oleifera Extracts”. Bitlis Eren Üniversitesi Fen Bilimleri Dergisi 15 (3): 1138-46. https://doi.org/10.17798/bitlisfen.1866992.

EndNote

Şahin B, Aktaş G (September 1, 2026) Determination of Phenolic Content and Antioxidant Activities of Moringa oleifera Extracts. Bitlis Eren Üniversitesi Fen Bilimleri Dergisi 15 3 1138–1146.

IEEE

[1]B. Şahin and G. Aktaş, “Determination of Phenolic Content and Antioxidant Activities of Moringa oleifera Extracts”, Bitlis Eren Üniversitesi Fen Bilimleri Dergisi, vol. 15, no. 3, pp. 1138–1146, Sept. 2026, doi: 10.17798/bitlisfen.1866992.

ISNAD

Şahin, Bihter - Aktaş, Gülsüm. “Determination of Phenolic Content and Antioxidant Activities of Moringa Oleifera Extracts”. Bitlis Eren Üniversitesi Fen Bilimleri Dergisi 15/3 (September 1, 2026): 1138-1146. https://doi.org/10.17798/bitlisfen.1866992.

JAMA

1.Şahin B, Aktaş G. Determination of Phenolic Content and Antioxidant Activities of Moringa oleifera Extracts. Bitlis Eren Üniversitesi Fen Bilimleri Dergisi. 2026;15:1138–1146.

MLA

Şahin, Bihter, and Gülsüm Aktaş. “Determination of Phenolic Content and Antioxidant Activities of Moringa Oleifera Extracts”. Bitlis Eren Üniversitesi Fen Bilimleri Dergisi, vol. 15, no. 3, Sept. 2026, pp. 1138-46, doi:10.17798/bitlisfen.1866992.

Vancouver

1.Bihter Şahin, Gülsüm Aktaş. Determination of Phenolic Content and Antioxidant Activities of Moringa oleifera Extracts. Bitlis Eren Üniversitesi Fen Bilimleri Dergisi. 2026 Sep. 1;15(3):1138-46. doi:10.17798/bitlisfen.1866992