Evaluation of Inhibition Effects of Some Cardiovascular Therapeutics on Human Erythrocyte Carbonic Anhydrase Isoenzymes

Namık Kılınç; Zuhal Alım; Bülent Şengül; Mehmet İşgör; Şükrü Beydemir

doi:10.17798/bitlisfen.345643

Evaluation of Inhibition Effects of Some Cardiovascular Therapeutics on Human Erythrocyte Carbonic Anhydrase Isoenzymes

Abstract

Carbonic anhydrase enzyme plays a vital role in metabolic events such as acid-base regulation and respiration. In our research, it is tried to determine the inhibitory influences of the cardiovascular therapeutics esmolol hydrochloride, amiodarone hydrochloride and lidocaine hydrochloride on human erythrocytes carbonic anhydrases (hCAI and II). In accordance with this purpose, carbonic anhydrase isoenzymes were purified from human erythrocytes by using affinity chromatography method. Enzyme purity was checked by SDS-PAGE electrophoresis method. After the aldose reductase enzymes were purified, the inhibitory affects of cardiovascular therapeutics on these enzymes, using esterase activity, which is the method of measuring in vitro activity, were examined. The three cardiovascular therapeutics dose-dependently decreased activity of hCAs. IC₅₀ values of amiodarone hydrochloride, esmolol hydrochloride and lidocaine hydrochloride were found to be, respectively, 0.91 mM, 5 mM, 5.8 mM for hCA-I and 0.41 mM, 3.5 mM, and 6.36 mM for hCA-II. Our results proved that, under in vitro conditions, cardiovascular therapeutics significantly inhibit human CA-I and II activities. So, irregular use of these medicines may cause serious adverse effects in terms of human health.

Keywords

Carbonic Anhydrase,Cardiovascular Therapeutics,Enzyme Inhibition

References

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Details

Primary Language

English

Subjects

-

Journal Section

Research Article

Authors

Namık Kılınç
IĞDIR ÜNİVERSİTESİ
Türkiye

Zuhal Alım
AHİ EVRAN ÜNİVERSİTESİ

Bülent Şengül This is me
BAYBURT ÜNİVERSİTESİ

Mehmet İşgör This is me
MUSTAFA KEMAL ÜNİVERSİTESİ

Şükrü Beydemir
ANADOLU ÜNİVERSİTESİ

Publication Date

June 29, 2018

Submission Date

October 22, 2017

Acceptance Date

January 25, 2018

Published in Issue

Year 2018 Volume: 7 Number: 1

DOI

https://doi.org/10.17798/bitlisfen.345643

IZ

https://izlik.org/JA74JP46EE

Cite

RIS / Bibtex

APA

Kılınç, N., Alım, Z., Şengül, B., İşgör, M., & Beydemir, Ş. (2018). Evaluation of Inhibition Effects of Some Cardiovascular Therapeutics on Human Erythrocyte Carbonic Anhydrase Isoenzymes. Bitlis Eren Üniversitesi Fen Bilimleri Dergisi, 7(1), 90-97. https://doi.org/10.17798/bitlisfen.345643

AMA

1.Kılınç N, Alım Z, Şengül B, İşgör M, Beydemir Ş. Evaluation of Inhibition Effects of Some Cardiovascular Therapeutics on Human Erythrocyte Carbonic Anhydrase Isoenzymes. Bitlis Eren Üniversitesi Fen Bilimleri Dergisi. 2018;7(1):90-97. doi:10.17798/bitlisfen.345643

Chicago

Kılınç, Namık, Zuhal Alım, Bülent Şengül, Mehmet İşgör, and Şükrü Beydemir. 2018. “Evaluation of Inhibition Effects of Some Cardiovascular Therapeutics on Human Erythrocyte Carbonic Anhydrase Isoenzymes”. Bitlis Eren Üniversitesi Fen Bilimleri Dergisi 7 (1): 90-97. https://doi.org/10.17798/bitlisfen.345643.

EndNote

Kılınç N, Alım Z, Şengül B, İşgör M, Beydemir Ş (June 1, 2018) Evaluation of Inhibition Effects of Some Cardiovascular Therapeutics on Human Erythrocyte Carbonic Anhydrase Isoenzymes. Bitlis Eren Üniversitesi Fen Bilimleri Dergisi 7 1 90–97.

IEEE

[1]N. Kılınç, Z. Alım, B. Şengül, M. İşgör, and Ş. Beydemir, “Evaluation of Inhibition Effects of Some Cardiovascular Therapeutics on Human Erythrocyte Carbonic Anhydrase Isoenzymes”, Bitlis Eren Üniversitesi Fen Bilimleri Dergisi, vol. 7, no. 1, pp. 90–97, June 2018, doi: 10.17798/bitlisfen.345643.

ISNAD

Kılınç, Namık - Alım, Zuhal - Şengül, Bülent - İşgör, Mehmet - Beydemir, Şükrü. “Evaluation of Inhibition Effects of Some Cardiovascular Therapeutics on Human Erythrocyte Carbonic Anhydrase Isoenzymes”. Bitlis Eren Üniversitesi Fen Bilimleri Dergisi 7/1 (June 1, 2018): 90-97. https://doi.org/10.17798/bitlisfen.345643.

JAMA

1.Kılınç N, Alım Z, Şengül B, İşgör M, Beydemir Ş. Evaluation of Inhibition Effects of Some Cardiovascular Therapeutics on Human Erythrocyte Carbonic Anhydrase Isoenzymes. Bitlis Eren Üniversitesi Fen Bilimleri Dergisi. 2018;7:90–97.

MLA

Kılınç, Namık, et al. “Evaluation of Inhibition Effects of Some Cardiovascular Therapeutics on Human Erythrocyte Carbonic Anhydrase Isoenzymes”. Bitlis Eren Üniversitesi Fen Bilimleri Dergisi, vol. 7, no. 1, June 2018, pp. 90-97, doi:10.17798/bitlisfen.345643.

Vancouver

1.Namık Kılınç, Zuhal Alım, Bülent Şengül, Mehmet İşgör, Şükrü Beydemir. Evaluation of Inhibition Effects of Some Cardiovascular Therapeutics on Human Erythrocyte Carbonic Anhydrase Isoenzymes. Bitlis Eren Üniversitesi Fen Bilimleri Dergisi. 2018 Jun. 1;7(1):90-7. doi:10.17798/bitlisfen.345643

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