The investigation of antioxidant and anticancer effects of some importance medical plants

Abstract

For thousands of years, plants have been used for the treatment of many diseases. In addition, in modern pharmacy many plant compounds have an important place in the production of pharmaceutical raw materials or new medicines. The aim of this study was to determine the biological activity of extracts obtained from the fruits of the medical important bitter melon (Momordica charantia), pepino (Solanum muricatum) and goldenberry (Physalis peruviana). The total polyphenol content of the samples was measured spectrophotometrically using Folin-Ciocaltute reactivity. The DPPH free radical scavenging effect of plant samples was determined and antioxidant capacities were determined. In addition, the cytotoxic effects of the samples on human over and breast cancer cell lines (A2780 and MCF7, respectively) were determined using MTT method. As a result of the study, it was determined that the highest total polyphenol content was in the case of potency and the lowest content was in the case of pepino. All three plant samples revealed the effect of free radical elimination of DPPH dependent predation. Finally, it was determined that all three plant samples showed high cytotoxic effect on both human over and breast cancer cell lines. Results show us that in all three plant samples it has a significantly higher anticancer effect than antioxidant effect. With the extensive studies to be carried out after that, the mechanism of the effect can be enlightened and contribute to new treatment approaches. 

Keywords

• Antioxidant,Cytotoxicity,Momordica charantia,Physalis peruviana,Solanum muricatum

Bazı önemli tıbbi bitkilerin antioksidan ve antikanser etkilerinin araştırılması

Abstract

Tıbbi açıdan önemli bitkiler binlerce yıldır birçok hastalığın tedavisi amacıyla kullanılmaktadır. Buna ek olarak modern eczacılıkta birçok bitki bileşiği ilaç ham maddesi veya yeni ilaçların yapımında önemli bir yere sahiptir. Bu çalışmada tıbbi öneme sahip kudret narı (Momordica charantia), pepino (Solanum muricatum) ve altın çilek (Physalis peruviana) bitkilerinin meyvelerinden elde edilen özütlerin biyolojik etkinliklerinin ortaya konulması amaçlanmıştır. Bitkilerin meyve özütleri %80’lik etanolde hazırlandıktan sonra toplam polifenol içeriği Folin-Ciocaltute reaktifi kullanılarak spektrofotometrik olarak ölçüldü. Bitki örneklerinin serbest radikal giderme etkisi DPPH serbest radikali kullanılarak belirlendi. Özütlerin insan over ve meme kanseri hücre hatları (sıyasıyla A2780 ve MCF7) üzerine sitotoksik etkileri MTT yöntemi kullanılarak belirlendi. Bitki örneklerindeki toplam polifenolik düzeyin en düşük 138,14±12,15 ve en yüksek 415,71±36,89 µg/gr gallik asit düzeyinde olduğu belirlendi. Bütün özütlerin düşük seviyede antioksidan etki sergilediği saptandı. Son olarak over ve meme kanseri hücre serilerine uygulanan özütlerin güçlü sitotoksik etki gösterdiği belirlendi (p<0.05). Sonuçta her üç bitki örneğinin de antioksidan etkilerinden ziyade yüksek antikanser etkiye sahip olduğunu bizlere göstermektedir. Her üç örneğinde söz konusu kanser türleri üzerine etkilerini aydınlatmak için ileriki çalışmalara ihtiyaç vardır.

Keywords

• Antioksidan,Sitotoksisite,Momordica charantia,Physalis peruviana,Solanum muricatum

References

1. Faydaoğlu E, Sürücüoğlu MS. 2011. Geçmişten günümüze tıbbi ve aromatik bitkilerin kullanılması ve ekonomik önemi, Kastamonu Üniversitesi Orman Fakültesi Dergisi, 11(1): 52-67.
2. Dar RA, Shahnawaz M, Qazi PH. 2017. General overview of medicinal plants: A review, The Journal of Phytopharmacology, 6(6): 349-51.
3. Singh R. 2015. Medicinal plants: A review, Journal of Plant Sciences, 3(1-1): 50-5.
4. Salehi B, Kumar NVA, Sener B, Sharifi-Rad M, Kilic M, Mahady GB, Vlaisavljevic S, Iriti M, Kobarfard F, Setzer WN, Ayatollahi SA, Ata A, Sharifi-Rad J. 2018. Medicinal Plants Used in the Treatment of Human Immunodeficiency Virus, Int J Mol Sci, 19(5).
5. Rehman S, Ashfaq UA, Ijaz B, Riazuddin S. 2018. Anti-hepatitis C virus activity and synergistic effect of Nymphaea alba extracts and bioactive constituents in liver infected cells, Microb Pathog, 121: 198-209.
6. Doaei S, Hajiesmaeil M, Aminifard A, Mosavi-Jarrahi SA, Akbari ME, Gholamalizadeh M. 2018. Effects of gene polymorphisms of metabolic enzymes on the association between red and processed meat consumption and the development of colon cancer; a literature review, J Nutr Sci, 7: e26.
7. Hamburger M, Hostettmann K. 1991. 7. Bioactivity in plants: the link between phytochemistry and medicine, Phytochemistry, 30(12): 3864-74.
8. Singleton VL, Rossi JA. 1965. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents, American journal of Enology and Viticulture, 16(3): 144-58.

9. Çöteli E, Erden Y, Karataş F. 2013. Yarpuz (Mentha pulegium L.) bitkisindeki malondialdehit, glutatyon ve vitamin miktarları ile total antioksidan kapasitesinin araştırılması, Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 17(2).
10. Brand-Williams W, Cuvelier ME, Berset C. 1995. Use of a free radical method to evaluate antioxidant activity, LWT - Food Science and Technology, 28(1): 25-30.
11. Tekin S, Erden Y, Sandal S, Yilmaz B. 2015. Is irisin an anticarcinogenic peptide?, Medicine Science, 4(2): 2172-80.
12. Mosmann T. 1983. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays, Journal of immunological methods, 65(1-2): 55-63.
13. Prinsloo G, Nogemane N, Street R. 2018. The use of plants containing genotoxic carcinogens as foods and medicine, Food Chem Toxicol, 116(Pt B): 27-39.
14. Gram H. 1993. Summary of WHO guidelines for the assessment of herbal medicines, Switzerland: World Health Organization: 13-4.
15. Güdr A. 2016. Influence of total anthocyanins from bitter melon (Momordica charantia Linn.) as antidiabetic and radical scavenging agents, Iranian journal of pharmaceutical research: IJPR, 15(1): 301.
16. Leelaprakash G, Rose JC, Gowtham B, Javvaji PK, Prasad S. 2011. In vitro antimicrobial and antioxidant activity of Momordica charantia leaves, Pharmacophore, 2(4): 244-52.
17. Sen S, Chakraborty R, Borah B, Dey B, Sarkar BR, Sahariah B. 2014. In vitro anthelmintic and antioxidant potential of fruits of Momordica charantia: A comparative study, Indian Journal of Health Sciences and Biomedical Research (KLEU), 7(2): 113.
18. Sudha G, Sangeetha Priya M, Indhu Shree RB, Vadivukkarasi S. 2012. Antioxidant activity of ripe and unripe pepino fruit (Solanum muricatum Aiton), Journal of food science, 77(11): C1131-C5.
19. Chang J, Lin C, Wu S, Lin D, Wang S, Miaw C, Ng L. 2008. Antioxidative and hepatoprotective effects of Physalis peruviana extract against acetaminophen-induced liver injury in rats, Pharmaceutical biology, 46(10-11): 724-31.
20. Koca N, Karadeniz F. 2005. Gıdalardaki doğal antioksidan bileşikler, Gıda/The Journal of Food, 30(4).
21. Cook N, Samman S. 1996. Flavonoids—chemistry, metabolism, cardioprotective effects, and dietary sources, The Journal of nutritional biochemistry, 7(2): 66-76.
22. Desai AG, Qazi GN, Ganju RK, El-Tamer M, Singh J, Saxena AK, Bedi YS, Taneja SC, Bhat HK. 2008. Medicinal plants and cancer chemoprevention, Current drug metabolism, 9(7): 581-91.
23. G Gutheil W, Reed G, Ray A, Anant S, Dhar A. 2012. Crocetin: an agent derived from saffron for prevention and therapy for cancer, Current pharmaceutical biotechnology, 13(1): 173-9.
24. Aggarwal BB, Shishodia S. 2006. Molecular targets of dietary agents for prevention and therapy of cancer, Biochem Pharmacol, 71(10): 1397-421.
25. Lee BM, Park KK. 2003. Beneficial and adverse effects of chemopreventive agents, Mutat Res, 523-524: 265-78.
26. Dia VP, Krishnan HB. 2016. BG-4, a novel anticancer peptide from bitter gourd (Momordica charantia), promotes apoptosis in human colon cancer cells, Sci Rep, 6: 33532.
27. Li CJ, Tsang SF, Tsai CH, Tsai HY, Chyuan JH, Hsu HY. 2012. Momordica charantia Extract Induces Apoptosis in Human Cancer Cells through Caspase- and Mitochondria-Dependent Pathways, Evid Based Complement Alternat Med, 2012: 261971.
28. Muhammad N, Steele R, Isbell TS, Philips N, Ray RB. 2017. Bitter melon extract inhibits breast cancer growth in preclinical model by inducing autophagic cell death, Oncotarget, 8(39): 66226-36.
29. Ren W, Tang DG. 1999. Extract of Solanum muricatum (Pepino/CSG) inhibits tumor growth by inducing apoptosis, Anticancer Res, 19(1A): 403-8.
30. Shathish K, Guruvayoorappan C. 2014. Solanum muricatum Ait. inhibits inflammation and cancer by modulating the immune system, J Cancer Res Ther, 10(3): 623-30.
31. Shathish K, Sakthivel KM, Guruvayoorappan C. 2015. Protective Effect of Solanum muricatum on Tumor Metastasis by Regulating Inflammatory Mediators and Nuclear Factor-Kappa B Subunits, J Environ Pathol Toxicol Oncol, 34(3): 249-62.
32. Wu SJ, Chang SP, Lin DL, Wang SS, Hou FF, Ng LT. 2009. Supercritical carbon dioxide extract of Physalis peruviana induced cell cycle arrest and apoptosis in human lung cancer H661 cells, Food Chem Toxicol, 47(6): 1132-8.
33. Lee CC, Chang WH, Chang YS, Liu TY, Chen YC, Wu YC, Chang JG. 2017. 4beta-Hydroxywithanolide E Modulates Alternative Splicing of Apoptotic Genes in Human Hepatocellular Carcinoma Huh-7 Cells, Sci Rep, 7(1): 7290.
34. Chang LC, Sang-Ngern M, Pezzuto JM, Ma C. 2016. The Daniel K. Inouye College of Pharmacy Scripts: Poha Berry (Physalis peruviana) with Potential Anti-inflammatory and Cancer Prevention Activities, Hawaii J Med Public Health, 75(11): 353-9.
35. Ang A, Pullar JM, Currie MJ, Vissers MCM. 2018. Vitamin C and immune cell function in inflammation and cancer, Biochem Soc Trans, 46(5):1147-1159.
36. Rengasamy KRR, Khan H, Gowrishankar S, Lagoa RJL, Mahomoodally FM, Khan Z, Suroowan S, Tewari D, Zengin G, Hassan STS, Pandian SK. 2018. The role of flavonoids in autoimmune diseases: Therapeutic updates, Pharmacol Ther.
37. Salehi B, Mishra AP, Nigam M, Sener B, Kilic M, Sharifi-Rad M, Fokou PVT, Martins N, Sharifi-Rad J. 2018. Resveratrol: A Double-Edged Sword in Health Benefits, Biomedicines, 6(3): e91.