Myrtus communis L. (Mersin) Yaprak Ekstraktının Genoprotektif Etkisinin Somatik Mutasyon ve Rekombinasyon Testi ile Değerlendirilmesi

Selda Öz; Şükran Çakır Arıca

doi:10.17798/bitlisfen.577518

Myrtus communis L. (Mersin) Yaprak Ekstraktının Genoprotektif Etkisinin Somatik Mutasyon ve Rekombinasyon Testi ile Değerlendirilmesi

Abstract

Bu çalışmada Myrtus communisL. (mersin) yaprak ekstraktının genoprotektif etkisi somatik mutasyon ve rekombinasyon testi (SMART) ile incelendi. Yüksek genotoksik etkiye sahip kemoterapötik bir ajan olan doksorubisin (DXR) pozitif kontrol olarak kullanıldı. Test maddeleri flare (flr³) ve çoklu kanat kılı (mwh) mutant işaret genlerini taşıyan üç günlük (72 ± 4 saat) transheterozigot Drosophila melanogaster larvalarına uygulandı. Mersin yaprak ekstraktı, genotoksik etkisini değerlendirmek için tek başına (1,5 ve 10 mg/mL) ve antigenotoksik etkisini değerlendirmek için doksorubisin (0,125 mg/ml) ile birlikte uygulandı. İnhibisyon yüzdeleri 1, 5 ve 10 mg/mL dozlarında sırasıyla %91.70, % 97.51 ve % 98.34 olarak hesaplandı. Bu çalışmadan elde edilen sonuçlar mersin yaprak ekstraktının test edilen tüm dozlarda doksorubisin kaynaklı mutant klon oluşumunu inhibe ederek antigenotoksik etki gösterdiğini ortaya koymuştur.

Keywords

Myrtus communis L.,antigenotoksisite,doksorubisin,Drosophila melanogaster

Supporting Institution

Kırıkkale Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi

Project Number

2012/010

Thanks

Bu çalışma Kırıkkale Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi tarafından desteklenmiştir (Proje No: 2012/010).

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Details

Primary Language

Turkish

Subjects

-

Journal Section

Research Article

Authors

Selda Öz ^*
0000-0003-1883-3441
Türkiye

Şükran Çakır Arıca This is me
0000-0003-1413-1143
Türkiye

Publication Date

September 30, 2019

Submission Date

June 13, 2019

Acceptance Date

August 20, 2019

Published in Issue

Year 2019 Volume: 8 Number: 3

DOI

https://doi.org/10.17798/bitlisfen.577518

IZ

https://izlik.org/JA68KJ82JZ

Cite

RIS / Bibtex

APA

Öz, S., & Çakır Arıca, Ş. (2019). Myrtus communis L. (Mersin) Yaprak Ekstraktının Genoprotektif Etkisinin Somatik Mutasyon ve Rekombinasyon Testi ile Değerlendirilmesi. Bitlis Eren Üniversitesi Fen Bilimleri Dergisi, 8(3), 875-882. https://doi.org/10.17798/bitlisfen.577518

AMA

1.Öz S, Çakır Arıca Ş. Myrtus communis L. (Mersin) Yaprak Ekstraktının Genoprotektif Etkisinin Somatik Mutasyon ve Rekombinasyon Testi ile Değerlendirilmesi. Bitlis Eren Üniversitesi Fen Bilimleri Dergisi. 2019;8(3):875-882. doi:10.17798/bitlisfen.577518

Chicago

Öz, Selda, and Şükran Çakır Arıca. 2019. “Myrtus Communis L. (Mersin) Yaprak Ekstraktının Genoprotektif Etkisinin Somatik Mutasyon Ve Rekombinasyon Testi Ile Değerlendirilmesi”. Bitlis Eren Üniversitesi Fen Bilimleri Dergisi 8 (3): 875-82. https://doi.org/10.17798/bitlisfen.577518.

EndNote

Öz S, Çakır Arıca Ş (September 1, 2019) Myrtus communis L. (Mersin) Yaprak Ekstraktının Genoprotektif Etkisinin Somatik Mutasyon ve Rekombinasyon Testi ile Değerlendirilmesi. Bitlis Eren Üniversitesi Fen Bilimleri Dergisi 8 3 875–882.

IEEE

[1]S. Öz and Ş. Çakır Arıca, “Myrtus communis L. (Mersin) Yaprak Ekstraktının Genoprotektif Etkisinin Somatik Mutasyon ve Rekombinasyon Testi ile Değerlendirilmesi”, Bitlis Eren Üniversitesi Fen Bilimleri Dergisi, vol. 8, no. 3, pp. 875–882, Sept. 2019, doi: 10.17798/bitlisfen.577518.

ISNAD

Öz, Selda - Çakır Arıca, Şükran. “Myrtus Communis L. (Mersin) Yaprak Ekstraktının Genoprotektif Etkisinin Somatik Mutasyon Ve Rekombinasyon Testi Ile Değerlendirilmesi”. Bitlis Eren Üniversitesi Fen Bilimleri Dergisi 8/3 (September 1, 2019): 875-882. https://doi.org/10.17798/bitlisfen.577518.

JAMA

1.Öz S, Çakır Arıca Ş. Myrtus communis L. (Mersin) Yaprak Ekstraktının Genoprotektif Etkisinin Somatik Mutasyon ve Rekombinasyon Testi ile Değerlendirilmesi. Bitlis Eren Üniversitesi Fen Bilimleri Dergisi. 2019;8:875–882.

MLA

Öz, Selda, and Şükran Çakır Arıca. “Myrtus Communis L. (Mersin) Yaprak Ekstraktının Genoprotektif Etkisinin Somatik Mutasyon Ve Rekombinasyon Testi Ile Değerlendirilmesi”. Bitlis Eren Üniversitesi Fen Bilimleri Dergisi, vol. 8, no. 3, Sept. 2019, pp. 875-82, doi:10.17798/bitlisfen.577518.

Vancouver

1.Selda Öz, Şükran Çakır Arıca. Myrtus communis L. (Mersin) Yaprak Ekstraktının Genoprotektif Etkisinin Somatik Mutasyon ve Rekombinasyon Testi ile Değerlendirilmesi. Bitlis Eren Üniversitesi Fen Bilimleri Dergisi. 2019 Sep. 1;8(3):875-82. doi:10.17798/bitlisfen.577518

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