Fluorescent Copper Phosphate Nanoflowers: A Novel Toxicity Investigation Study Based On Tenebrio Molitor Linnaeus, 1758 (Coleoptera: Tenebrionidae) Larvae

Abstract

Organic-inorganic hybrid nanoflowers with commercialization potential are the novel materials and are widely used in many experimental applications, recently. However their potential toxicity values of these nanoflowers are still unknown. In this study, fluorescent copper phosphate nanoflowers (FCPnfs) were first synthesized to constructed via organic-inorganic hybrid nanoflower synthesis method at Science Technology Application and Research Center Laboratory of Nevsehir Haci Bektas Veli University in 2018. The morphological features of FCPnfs (acridine orange amount, shape, size) were analyzed. The results showed that different amount of acridine orange caused different morphology and the organic component were homogeneously distributed inside the nanoflowers. These materials were characterized by SEM, EDX, XRD, and FTIR techniques. The toxic effects of the as-prepared FCPnfs were investigated in Tenebrio molitor Linnaeus, 1758 (Coleoptera: Tenebrionidae) larvae compared to pure copper phosphate nanoflowers (CPnfs) at Plant and Animal Production Department Laboratory of Nevsehir Haci Bektas Veli University, Avanos Vocational School in 2018. Nanoflowers were force feed to larvae at the different doses of 0.001, 0.005, 0.010, 0.025, 0.050, 0.100 mg/10μl. According to probit assay, LC₅₀ and LC₉₉ values of FCPnfs were found 0.490 and 0.145 mg/10μl, respectively. On the other hand, LC₅₀ and LC₉₉ values of CPnfs were detected 0.066 and 0.172 mg/10μl, respectively. It was found that the insect exhibited slightly more resistance to CPnfs than FCPnfs when compared to both chemical toxic values. These new findings will be offer a new insight into how to design new materials in industrial uses.

Keywords

• Copper phosphate; Nanoflowers; Toxic effects.

Tenebrio molitor larvasında gerçekleştirilen yeni bir toksik araştırma: Floresan bakır fosfat nano çiçekler

Abstract

Son zamanlarda birçok deneysel uygulamada yaygın olarak kullanılan organik-inorganik hibrit nano çiçekler ticarileşme potansiyeline sahip yeni malzemelerdir. Ancak bu nano çiçeklerin toksik etkileri hala bilinmemektedir. Bu çalışmada, ilk defa organik-inorganik hibrit nano çiçek sentez metodu kullanılarak floresan bakır fosfat nano çiçekler (FCPnfs) 2018 yılında Nevşehir Hacı Bektaş Veli Üniversitesi, Bilim Teknoloji Araştırma ve Uygulama Merkezi laboratuvarında sentezlendi. FCPnfs'in morfolojik özellikleri (akridin orange miktarı, şekli, boyutu) analiz edildi. Sonuçlar, farklı miktarda akridin orange’ın farklı morfolojiye neden olduğu ve organik bileşenin nano çiçeklerin içinde homojen bir şekilde dağıldığını gösterdi. Sentezlenen malzemelerin karakterizasyonları SEM, EDX, XRD ve FTIR teknikleri ile gerçekleştirildi. Model organizma olarak seçilen Tenebrio molitor Linnaeus, 1758 (Coleoptera: Tenebrionidae) larvalarında sentezlenen FCPnfs'lerin toksik etkileri, saf bakır fosfat nano çiçekleriyle (CPnfs) karşılaştırmalı olarak 2018 yılında Nevşehir Hacı Bektaş Veli Üniversitesi, Avanos Meslek Yüksekokulu, Bitkisel ve Hayvansal Üretim Laboratuvarı’nda incelendi. Larvalara nano çiçekler, 0.001, 0.005, 0.010, 0.025, 0.050, 0.100 mg/10µl olacak şekilde farklı dozlarda zorla besleme yapıldı. Yapılan probit analizine göre, FCPnfs'in LC₅₀ değeri 0.490 mg/10µl iken LC₉₉ değeri 0.145 mg/10µl bulundu. Öte yandan, CPnfs'nin LC₅₀ değeri 0.066 mg/10µl iken LC₉₉ değeri 0.172 mg/10µl bulundu. Elde edilen toksik veriler değerlendirildiğinde; Tenebrio molitor larvalarının CPnfs'ye FCPnfs'den biraz daha fazla direnç gösterdiği bulundu. Bu bulgular, endüstriyel kullanımlarda yeni malzemelerin nasıl tasarlanacağı hakkında yeni bir bakış açısı kazandıracaktır.

Keywords

• Bakır fosfat; Nano çiçekler; Toksik etki.

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