Heavy Metal Tolerance Potential of Aspergillus alliaceus Isolated from a Green Turtle Nesting Site

Yıl 2021, , 49 - 56, 21.03.2021

Esra Deniz Candan

https://doi.org/10.17798/bitlisfen.830337

Öz

Heavy metals are naturally present in the environment. As a result of human activities and some natural events, mixing of these metals in water, air and soil is one of the most serious global problems. The toxicity of these metals is also a serious global problem due to their accumulation in living things and non-biodegradable characteristics. Increasing heavy metal pollution in aquatic and terrestrial environments requires improvement strategies such as bioremediation to remove these metals from the environment for human and environmental health. In this study, fungal isolation was performed to examine fungi with heavy metal remediation potential using sand samples from Sugözü Beaches where are subjected to intense environmental effects due to human activities including industrial facilities and marine traffic. This beach is also an important nesting area for the green turtle. Aspergillus alliaceus isolated from this beach was identified using primers of internal transcribed spacer 1 and 4. To determine the tolerance of A. alliaceus to iron, zinc, cobalt and copper, the fungus was inoculated into media containing metal at different concentrations (200, 400, 600, 800, and 1000 ppm). Daily mycelium growths were recorded during a ten day incubation at 30°C. A.alliaceus was found to tolerate Fe and Zn at all concentrations. Besides, it tolerated Co and Cu up to 400 and 600 ppm, respectively. In this study, the tolerance of A. alliaceus to Fe, Zn, and Co was determined for the first time and it is recommended that it can be used efficiently in bioremediation studies.

Anahtar Kelimeler

Aspergillus alliaceus, Chelonia mydas, Heavy metal, Metal tolerance

Proje Numarası

FEN-BAP-A-150219-11

Kaynakça

• Järup L. 2003. Hazards of Heavy Metal Contamination. British Medical Bulletin, 68 (1): 167-182.
• Morais S., Costa F.G., Pereira M.D.L. 2012. Heavy Metals and Human Health. Environmental Health–Emerging Issues and Practice, 10: 227-246.
• Tchounwou P.B., Yedjou C.G., Patlolla A.K., Sutton D.J. 2012. Heavy Metal Toxicity and the Environment. In: Molecular, Clinical and Environmental Toxicology, Edited by Luch A., Vol: 5, Experientia Supplementum, Springer, Basel, 133-164.
• Duruibe J.O., Ogwuegbu M.O.C., Egwurugwu J.N. 2007. Heavy Metal Pollution and Human Biotoxic Effects. International Journal of Physical Sciences, 2 (5): 112-118.
• Rehman A.U., Nazir S., Irshad R., Tahir K., urRehman K., Islam R.U., Wahab Z. 2020. Toxicity of Heavy Metals in Plants and Animals and Their Uptake by Magnetic Iron Oxide Nanoparticles. Journal of Molecular Liquids, 114455.
• Jan A.T., Azam M., Siddiqui K., Ali A., Choi I., Haq Q.M. 2015. Heavy Metals and Human Health: Mechanistic Insight into Toxicity and Counter Defense System of Antioxidants. International Journal of Molecular Sciences, 16 (12): 29592-29630.
• Zotti M., Di Piazza S., Roccotiello E., Lucchetti G., Mariotti M.G., Marescotti P. 2014. Microfungi in Highly Copper-Contaminated Soils from an Abandoned Fe–Cu Sulphide Mine: Growth Responses, Tolerance and Bioaccumulation. Chemosphere, 117: 471-476.
• Jacob J.M., Karthik C., Saratale R.G., Kumar S.S., Prabakar D., Kadirvelu K., Pugazhendhi A. 2018. Biological Approaches to Tackle Heavy Metal Pollution: A Survey of Literature. Journal of Environmental Management, 217: 56-70.
• Carrillo-González R., González-Chávez M.D.C.A. 2012. Tolerance to and Accumulation of Cadmium by the Mycelium of the Fungi Scleroderma citrinum and Pisolithustinctorius. Biological Trace Element Research, 146 (3): 388-395.
• Ceci A., Maggi O., Pinzari F., Persiani A.M. 2012. Growth Responses to and Accumulation of Vanadium in Agricultural Soil Fungi. Applied Soil Ecology, 58: 1-11.
• Zafar S., Aqil F., Ahmad I. 2007. Metal Tolerance and Biosorption Potential of Filamentous Fungi Isolated from Metal Contaminated Agricultural Soil. Bioresource Technology, 98 (13): 2557-2561.
• Valix M., Tang J.Y., Malik R. 2001. Heavy Metal Tolerance of Fungi. Minerals Engineering, 14 (5): 499-505.
• Anahid S., Yaghmaei S., Ghobadinejad Z. 2011. Heavy Metal Tolerance of Fungi. ScientiaIranica, 18 (3): 502-508.
• Iskandar N.L., Zainudin N.A.I.M., Tan S.G. 2011. Tolerance and Biosorption of Copper (Cu) and Lead (Pb) by Filamentous Fungi Isolated from a Freshwater Ecosystem. Journal of Environmental Sciences, 23 (5): 824-830.
• Ojuederie O.B., Babalola O.O. 2017. Microbial and Plant-Assisted Bioremediation of Heavy Metal Polluted Environments: A Review. International Journal of Environmental Research and Public Health, 14 (12): 1504.
• Rose P.K., Devi R. 2018. Heavy Metal Tolerance and Adaptability Assessment of Indigenous Filamentous Fungi Isolated from Industrial Wastewater and Sludge Samples. Beni-Suef University Journal of Basic and Applied Sciences, 7 (4): 688-694.
• Doygun H., Alphan H. 2006. Monitoring Urbanization of Iskenderun, Turkey, and Its Negative Implications. Environmental Monitoring and Assessment, 114 (1-3): 145-155.
• Yilmaz A.B. 2003. Levels of Heavy Metals (Fe, Cu, Ni, Cr, Pb, And Zn) in Tissue of Mugil cephalus and Trachurus mediterraneus from Iskenderun Bay, Turkey. Environmental Research, 92 (3): 277-281.
• Cevik U., Koz B., Makarovska Y. 2010. Heavy Metal Analysis Around Iskenderun Bay in Turkey. X‐Ray Spectrometry: An International Journal, 39 (3): 202-207.
• Yılmaz A.B., Sangün M.K., Yağlıoğlu D., Turan C. 2010. Metals (Major, Essential to Non-Essential) Composition of The Different Tissues of Three Demersal Fish Species from Iskenderun Bay, Turkey. Food Chemistry, 123 (2): 410-415.
• Çiftçi N., Ayas D., Bakan M. 2020. The Comparison of Heavy Metal Level in Surface Water, Sediment and Biota Sampled from the Polluted and Unpolluted Sites in the Northeastern Mediterranean Sea. Thalassas: An International Journal of Marine Sciences, 1-12.
• Duysak Ö., Uğurlu E. 2020. Trace Metal Concentrations in the Seston of the Gulf of İskenderun (Turkey, North-Eastern Mediterranean). Thalassas: An International Journal of Marine Sciences, 36 (1): 125-132.
• Canbolat A.F., Atatunç K., Candan O., Barcak D. 2005. A New Green Turtle (Chelonia mydas) Nesting Site in The Mediterranean: Sugözü Beaches, Adana (Turkey). The Second Mediterranean Conference on Sea Turtles, Book of Abstracts, 4-7 May, Antalya, 65.
• Seminoff J.A. 2004. Chelonia mydas. The IUCN Red List of Threatened Species 2004: e. T4615A11037468.
• Anan Y., Kunito T., Sakai H., Tanabe S. 2002. Subcellular Distribution of Trace Elements in The Liver of Sea Turtles. Marine Pollution Bulletin, 45 (1-12): 224-229.
• Sinaei M., Bolouki M. 2017. Metals in Blood and Eggs of Green Sea Turtles (Chelonia mydas) from Nesting Colonies of the Northern Coast of the Sea of Oman. Archives of Environmental Contamination and Toxicology, 73 (4): 552-561.
• Candan O., Candan E.D. 2020. Bacterial Diversity of the Green Turtle (Chelonia mydas) Nest Environment. Science of The Total Environment, 720: 137717.
• White T.J., Bruns T., Lee S.J.W.T., Taylor J. 1990. Amplification and Direct Sequencing of Fungal Ribosomal RNA Genes for Phylogenetics. PCR protocols: A guide to methods and Applications, 18 (1): 315-322.
• Oladipo O.G., Awotoye O.O., Olayinka A., Ezeokoli O.T., Maboeta M.S., Bezuidenhout C.C. 2016. Heavy Metal Tolerance Potential of Aspergillus Strains Isolated from Mining Sites. Bioremediation Journal, 20 (4): 287-297.
• Gavrilescu M. 2004. Removal of Heavy Metals from the Environment by Biosorption. Engineering in Life Sciences, 4 (3): 219-232.
• Mukherjee A., Das D., Mondal S.K., Biswas R., Das T.K., Boujedaini N., Khuda-Bukhsh A.R. 2010. Tolerance of Arsenate-Induced Stress in Aspergillus niger, A Possible Candidate for Bioremediation. Ecotoxicology and Environmental Safety, 73 (2): 172-182.
• Tchounwou P.B., Newsome C., Williams J., Glass K. 2008. Copper-induced cytotoxicity and transcriptional activation of stress genes in human liver carcinoma (HepG2) cells. Metal Ions in Biology and Medicine, 10: 285-290.
• Kushwaha A., Rani R., Kumar S., Gautam A. 2015. Heavy Metal Detoxification and Tolerance Mechanisms in Plants: Implications for Phytoremediation. Environmental Reviews, 24 (1): 39-51.
• Vuori K.M. 1995. Direct and indirect effects of iron on river ecosystems. In Annales Zoologici Fennici, 32: 317-329.
• McKinley K., McLellan I., Gagné F., Quinn B. 2019. The Toxicity of Potentially Toxic Elements (Cu, Fe, Mn, Zn and Ni) to the Cnidarian Hydra attenuata at Environmentally Relevant Concentrations. Science of the Total Environment, 665: 848-854.
• Muñoz A.J., Ruiz E., Abriouel H., Gálvez A., Ezzouhri L., Lairini K., Espínola F. 2012. Heavy Metal Tolerance of Microorganisms Isolated from Wastewaters: Identification and Evaluation of Its Potential for Biosorption. Chemical Engineering Journal, 210: 325-332.
• Iram S., Zaman A., Iqbal Z., Shabbir R. 2013. Heavy Metal Tolerance of Fungus Isolated from Soil Contaminated with Sewage and Industrial Wastewater. Polish Journal of Environmental Studies, 22: 3.