Evaluation of Inhibition Effects of Some Cardiovascular Therapeutics on Human Erythrocyte Carbonic Anhydrase Isoenzymes

Yıl 2018, Cilt: 7 Sayı: 1, 90 - 97, 29.06.2018

Namık Kılınç , Zuhal Alım , Bülent Şengül Mehmet İşgör Şükrü Beydemir

https://doi.org/10.17798/bitlisfen.345643

Cited By: 3

Öz

Abstract

Carbonic
anhydrase enzyme plays a vital role in metabolic events such as acid-base
regulation and respiration. In our research, it is tried to determine the
inhibitory influences of the cardiovascular therapeutics esmolol hydrochloride,
amiodarone hydrochloride and lidocaine hydrochloride on human erythrocytes
carbonic anhydrases (hCAI and II). In accordance with this purpose, carbonic
anhydrase isoenzymes were purified from human erythrocytes by using affinity
chromatography method. Enzyme purity was checked by SDS-PAGE electrophoresis
method. After the aldose reductase enzymes were purified, the inhibitory
affects of cardiovascular therapeutics on these enzymes, using esterase
activity, which is the method of measuring in
vitro activity, were examined. The three cardiovascular therapeutics
dose-dependently decreased activity of hCAs. IC₅₀ values of
amiodarone hydrochloride, esmolol hydrochloride and lidocaine hydrochloride
were found to be, respectively, 0.91 mM, 5 mM, 5.8 mM for hCA-I and 0.41 mM,
3.5 mM, and 6.36 mM for hCA-II. Our results proved that, under in vitro conditions, cardiovascular
therapeutics significantly inhibit human CA-I and II activities. So, irregular
use of these medicines may cause serious adverse effects in terms of human
health.

Anahtar Kelimeler

Carbonic Anhydrase, Cardiovascular Therapeutics, Enzyme Inhibition

Kaynakça

• 1. Supuran, C.T. 2008. Carbonic Anhydrases: Novel Therapeutic Applications for Inhibitors and Activators, Nature Reviews Drug Discovery, 7(2), 168–181.
• 2. Temperini, C., Innocenti, A., Scozzafava, A., Parkkila, S., Supuran, C.T. 2010. The Coumarin-binding Site in Carbonic Anhydrase Accommodates Structurally Diverse Inhibitors: The Antiepileptic Lacosamide As an Example and Lead Molecule for Novel Classes of Carbonic Anhydrase Inhibitors, Journal of Medicinal Chemistry, 53(2), 850-854.
• 3. Ekinci, D., Beydemir, S., Alim, Z. 2007. Some Drugs Inhibit In Vitro Hydratase and Esterase Activities of Human Carbonic Anhydrase-I and II, Pharmacological Reports, 59(5), 580-587.
• 4. Ekinci, D., Al-Rashida, M., Abbas, G., Senturk, M., Supuran, C.T. 2012. Chromone Containing Sulfonamides as Potent Carbonic Anhydrase Inhibitors, Journal of Enzyme Inhibition and Medicinal Chemistry, 27(5), 744–747.
• 5. Ridderstrale, Y., Wistrand, P.J., William, F.B. 1994. Membrane-associated CA Activity in The Eye of The CA II-Deficient Mouse, Investigative Ophthalmology & Visual Science, 35(5), 2577-2584.
• 6. Parkkila, S., Rajaniemi, H., Parkkila, A.K., Kivela, J., Waheed, A., Pastoreková, S., Pastorek, J., Sly, W.S. 2000. Carbonic Anhydrase Inhibitor Suppresses Invasion of Renal Cancer Cells In-Vitro, Proceedings of the National Academy of Sciences, 97(5), 2220-2224.
• 7. Hynninen, P., Parkkila, S., Huhtala, H., Pastorekova, S., Pastorek, J., Waheed, A., Sly, W.S., Tomas, E. 2012. Carbonic Anhydrase İsozymes II, IX, And XII in Uterine Tumors. APMIS, 120(2), 117-129.
• 8. Yoshiura, K., Nakaoka, T., Nishishita, T., Sato, K., Yamamoto, A., Shimada, S., Saida, T., Kawakami, Y., Takahashi, T.A., Fukuda, H., Imajoh-Ohmi, S., Oyaizu, N., Yamashita, N. 2005. Carbonic Anhydrase II is a Tumor Vessel Endothelium-associated Antigen Targeted by Dendritic Cell Therapy, Clinical Cancer Research, 11(22), 8201-8207.
• 9. Parkkila, S., Lasota, J., Fletcher, J.A., Ou, W.B., Kivelä, A.J., Nuorva, K., Parkkila, A., Ollikainen, J., Sly, W.S., Waheed, A., Pastorekova, S., Pastorek, J., Isola, J., Miettinen, M. 2010. Carbonic Anhydrase II. a Novel Biomarker for Gastrointestinal Stromal Tumors, Modern Pathology, 23 (5), 743-50.
• 10. Desmond, J., Steven, S., Edward, M. 1993. Esmolol Hydrochloride, Sodium Nitroprusside, and Isoflurane Differ in Their Ability to Alter Peripheral Sympathetic Responses, Anesthesia & Analgesia, 77(2), 281-290.
• 11. Swan, J.H. and Chisholm, A.W. 1976. Control of Recurrent Supraventricular Tachycardia with Amiodarone Hydrochloride, Canadian Medical Association Journal, 114(1), 43–44.
• 12. Claffey, E., Reader, A., Nusstein, J., Beck, M., Weaver, J. 2004. Anesthetic Efficacy of Articaine for Inferior Alveolar Nerve Blocks in Patients with Irreversible Pulpitis, Journal of Endodontics, 30 (8), 568-571.
• 13. Wilbur, K.M. and Anderson, N.G. 1948. Electrometric and Colorimetric Determination of Carbonic Anhydrase, Journal of Biological Chemistry, 176 (1), 147–154.
• 14. Verpoorte, J.A., Mehta, S., Edsall, J.T. 1967. Esterase Activities of Human Carbonic Anhydrases B And C, Journal of Biological Chemistry, 242(18), 4221–4229.
• 15. Alim, Z., Kilinc, N., Isgor, M.M., Sengul, B., Beydemir, S. 2015. Some Anti-inflammatory Agents Inhibit Esterase Activities of Human Carbonic Anhydrase Isoforms I and II: An In Vitro Study, Chemical Biology & Drug Design, 86 (4), 857–863.
• 16. Bradford, M.M. 1976. A Rapid and Sensitive Method for The Quantitation of Microgram Quantities of Protein Utilizing The Principle of Protein-dye Binding, Analytical Biochemistry, 72(1-2), 248–251.
• 17. Laemmli, U.K. 1970. Cleavage of Structural Proteins During The Assembly of The Head of Bacteriophage T4, Nature, 227(5259), 680–685.
• 18. Alim, Z. and Beydemir, S. 2016. Some Anticancer Agents Act on Human Serum Paraoxonase‐1 to Reduce Its Activity, Chemical Biology & Drug Design, 88(2), 188-196.
• 19. Sen, E., Alım, Z., Duran, H., İsgör, M.M., Beydemir, S., Kasımogulları, R., Ok, S. 2013. Inhibitory Effect of Novel Pyrazole Carboxamide Derivatives on Human Carbonic Anhydrase Enzyme, Journal of Enzyme Inhibition and Medicinal Chemistry, 28(2), 328-336.
• 20. Ekinci, D. and Beydemir, S. 2009. Effect of Some Analgesics on Paraoxonase-1 Purified from Human Serum, Journal of Enzyme Inhibition and Medicinal Chemistry, 24(4), 1034–1039.