Evaluation of Inhibition Effects of Some Cardiovascular Therapeutics on Human Erythrocyte Carbonic Anhydrase Isoenzymes

Yıl 2018, Cilt: 7 Sayı: 1, 90 - 97, 29.06.2018

Namık Kılınç Zuhal Alım Bülent Şengül Mehmet İşgör Şükrü Beydemir

https://doi.org/10.17798/bitlisfen.345643

Cited By: 3

Öz

Abstract

Carbonic
anhydrase enzyme plays a vital role in metabolic events such as acid-base
regulation and respiration. In our research, it is tried to determine the
inhibitory influences of the cardiovascular therapeutics esmolol hydrochloride,
amiodarone hydrochloride and lidocaine hydrochloride on human erythrocytes
carbonic anhydrases (hCAI and II). In accordance with this purpose, carbonic
anhydrase isoenzymes were purified from human erythrocytes by using affinity
chromatography method. Enzyme purity was checked by SDS-PAGE electrophoresis
method. After the aldose reductase enzymes were purified, the inhibitory
affects of cardiovascular therapeutics on these enzymes, using esterase
activity, which is the method of measuring in
vitro activity, were examined. The three cardiovascular therapeutics
dose-dependently decreased activity of hCAs. IC₅₀ values of
amiodarone hydrochloride, esmolol hydrochloride and lidocaine hydrochloride
were found to be, respectively, 0.91 mM, 5 mM, 5.8 mM for hCA-I and 0.41 mM,
3.5 mM, and 6.36 mM for hCA-II. Our results proved that, under in vitro conditions, cardiovascular
therapeutics significantly inhibit human CA-I and II activities. So, irregular
use of these medicines may cause serious adverse effects in terms of human
health.

Anahtar Kelimeler

Carbonic Anhydrase, Cardiovascular Therapeutics, Enzyme Inhibition

Kaynakça

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