HPLC Kullanarak Bazı Astragalus Türlerinin Yağda Çözünen Vitamin İçerikleri

Yıl 2019, Cilt: 8 Sayı: 1, 84 - 89, 12.03.2019

Muammer Bahşi Murat Kürşat İrfan Emre Ökkeş Yılmaz

https://doi.org/10.17798/bitlisfen.508433

Öz

Bu çalışmanın amacı, HPLC analizlerine göre lipitte çözünen vitamin
içeriklerini belirleyerek beş Astragalus türünün
(Astragalus
anthlloides,  Astragalus hirsutus,  Astragalus campylorhynchus, Astragalus
cephalotes var. cephalotes, Astragalus odaratus) biyokimyasal çalışmalarına katkıda bulunmaktır. Bu
çalışma, A. anthlloides (208,95±4,09
µg/g) ve A. hirsutus (200,9±2,8 µg/g’un
yüksek ϒ-tocopherol içeriğine sahip olduğunu göstermiştir. Ayrıca,
çalışılan Astragalus türlerinin D3
vitamin içerikleri 2,94±0,18 µg/g (Astragalus
odaratus) ve 12,53±,495 µg/g (Astragalus
anthlloides) arasında bulunmuştur. Öte yandan, Astragalus türlerinin α-tokoferol içerikleri 3,99±0,14 µg/g (Astragalus campylorhynchus) ile
11,9±0,66 µg/g (Astragalus cephalotes var.
cephalotes) arasında belirlenmiştir.
Bununla beraber, çalışılan türler arasında sadece Astragalus hirsutus’un beta karoten içeriğine (3,55±0,43 µg/g)  sahip olduğu bulunmuştur. Ayrıca,
r-tokoferol, a-tokoferol asetat, D2, K1, retinol ve retinol asetat
içeriklerinin ya çok düşük oranlarda olduğu ya da hiç bulunmadığı bulunmuştur. 

Anahtar Kelimeler

Astragalus, HPLC, lipitte çözünen vitaminler

The Lipide- Soluble Vitamin Contents Of Some Astragalus Taxa By Using HPLC

Öz

The aim of this study is to contribute the biochemical studies of five
Astragalus (Astragalus anthlloides,  Astragalus hirsutus,  Astragalus campylorhynchus, Astragalus
cephalotes var.
cephalotes, Astragalus odaratus) species by determining the lipide-soluble
vitamin contents based on HPLC analyse. Current study showed that A. anthlloides (208,95±4,09 µg/g) and A.
hirsutus (200,9±2,8 µg/g) have
high ϒ-tocopherol content. Also, D3 vitamin content of studied Astragalus species were found between
2,94±0,18 µg/g (Astragalus odaratus)
and 12,53±,495 µg/g (Astragalus
anthlloides). On the other hand, α-tocopherol content of Astragalus species have detected between
3,99±0,14 µg/g (Astragalus
campylorhynchus) and 11,9±0,66 µg/g (Astragalus
cephalotes var. cephalotes).
However, it was found that A. hirsutus only have beta caroten content (3,55±0,43
µg/g) among studied species. On the
other hand, it was found that r-tocopherol, a-tocopherol acetate, D2, K1,
retinol and retinol acetate contents 
were lowest amounts or absent. 

Anahtar Kelimeler

Astragalus, HPLC, lipide-soluble vitamins.

Kaynakça

• 1. Chamberlain D.F., Mathews M.A. 1970. Astragalus L. In: Davis PH (ed.). Flora of Turkey and the East Aegean Islands, Vol. 3. UK: Edinburgh University Press, pp. 49 Edinburgh.
• 2. Duran A., Aytac Z. 2005. Astragalus nezaketae (Fabaceae), a new species from Turkey, Ann. Bot. Fennici. 42: 381–385.
• 3. Martin E., Duran A., Dinc M., Erisen S. 2008. Karyotype Analyses of Four Astragalus L. (Fabaceae) Species From Turkey. Phytologia, 90(2); 147-159.
• 4. Podlech D. 1986. Taxonomic and phytogeographical problems in Astragalus of the Old World and South-West Asia. P Roy Soc Edinb B 89: 37-43.
• 5. Cetin O., Martin E., Duran A., Ozdemir A. 2010. Karyological study on endemic Astragalus stereocalyx Bornm. (Milk-vetch) in Turkey. Biological Diversity and Conservation, 3/3, 153-157.
• 6. Keskin C., Kacar S. 2013. Fatty acid composition of root and shoot samples of some Astragalus L. (Fabaceae) taxa growing in the east and southeast of Turkey. Turk J. Biol. 37: 122-128.
• 7. Jaradat N.A., Zaid A.N., Abuzant A., Khalaf S., Abu-Hassan N. 2017. Phytochemical and biological properties of four Astragalus species commonly used in traditional Palestinian medicine. European Journal of Integrative Medicine 9, 1–8.
• 8. Podlech D. 1999. New Astragali and Oxytropis from North Africa and Asia, including some new combinations and remarks on some species. Sendtnera 6: 135–171.
• 9. Agar G., Bozari S., Adiguzel A., Baris O., Gulluce M., Sengul M., Sahin F. 2009. Phenotypic and genetic variation among Astragalus species from Turkey. Romanian Biotechnological Letters. Vol. 14, No. 2, pp. 4267-4274.
• 10. Bellakhdar J.1997. La Pharmacopée Marocaine Traditionnelle, Ibis Press, Paris, France.
• 11. Kumar S., Sephuhle N., Bouic P.J., Rosenkranz B. 2018. HPLC/LC-MS Guided Phytochemical and In vitro Screening of Astragalus membranaceus (Fabaceae), and Prediction of Possible Interactions with CYP2B6, Journal of Herbal Medicine, vol.14, p 35-47. https://doi.org/10.1016/j.hermed.2018.10.008.
• 12. Arumungam R., Kirkan B., Sarikurkcu C. 2018. Phenolic profile, antioxidant and enzyme inhibitory potential of methanolic extracts from different parts of Astragalus ponticus Pall. South African Journal of Botany in press. https://doi.org/10.1016/j.sajb.2018.07.002.
• 13. Liu Y., Liu J., Wang Y., Abozeid A., Tang Z.H. 2016. Simultaneous determination of six active metabolites in Astragalus mongholicus (Fisch.) Bge. Under salt stress by ultra-pressure liquid chromatography with tandem mass spectrometry, 5, 927.
• 14. Ionkova I., Shkondrov A., Krasteva I., Ionkov T. 2014. Recent progress in phytochemistry, pharmacology and biotechnology of Astragalus saponins. Phytochemistry Reviews 13(2):343-374. DOI: 10.1007/s11101-014-9347-3.
• 15. Bratkov V., Shkondrov A., Zdraveva P., Krasteva I. 2016. Flavonoids from the genus Astragalus: phytochemistry and biological activity. Pharmacogn. Rev. 10, 11.http:// dx.doi.org/10.4103/0973-7847.176550.
• 16. Krasteva I., Shkondrov A., Ionkova I., Zdraveva P. 2016. Advances in phytochemistry, pharmacology and biotechnology of Bulgarian Astragalus species. Phytochem. Rev 15 (4), 567–590.http://dx.doi.org/10.1007/s11101-016-9462-4.
• 17. Liu Y., Liu J., Wu K.X., Guo X.R., Tang Z.H. 2018. A rapid method for sensitive profiling of bioactive triterpene and flavonoid from Astragalus mongholicus and Astragalus membranaceusby ultra-pressure liquid chromatography with tandem mass spectrometry. Journal of Chromatography B 1085; 110–118.
• 18. Shkondrov A., Krasteva I., Bucar F., Kunert O., Kondeva-Burdina M., Ionkova I. 2018. Flavonoids and saponins from two Bulgarian Astragalus species and their neuroprotective activity. Phytochemistry Letters 26; 44–49.
• 19. Balch P. 2006. Prescription for Nutritional Healing (4th Ed.). Avery Penguin Putnam. ISBN 978-1-58333-236-8.
• 20. Ko J.K., Chik C.W. 2009. The protective action ofradix Astragalus membranaceus against hapten induced colitis through modulation of cytokines. Cytokine 47;85–90.
• 21. Guo K., He X., Zhang Y., Li X., Yan Z., Pan L., Qin B. 2016. Flavoniods from aerial parts of Astragalus hoantchy. Fitoterapia 114, 34–39.
• 22. Shang H., Chen S., Li R., Zhou H., Wu H., Song H., 2018. Influences of extraction methods on physicochemical characteristics and activities of Astragalus cicer L. polysaccharides. Process Biochemistry 73; 220–227.
• 23. Hara A., Radin N.S. 1978. Lipid extraction of tissues with a low-toxicity solvent. Anal.Biochem. 90 (1) 420-426.
• 24. Sanchez-Machado D.I., Lopez-Hernandez J., Paseiro-Losado P. 2002. High-performance liquid chromatographic determination of a-tocopherol in macroalgae. Journal of Chromatography A 976 (1) 277–284.
• 25. Yılmaz O., Keser S., Tuzcu M., Cetintas B. 2007. Resveratrol (trans-3,4’,5-trihydoxystilbene) decreases lipid peroxidation level and protects antioxidant capacity in sera and erytrocytes of old female Wistar rats induced by the kidney carcinogen potassium bromate. Envir. Toxicol. Pharmacol., 24, 79-85.
• 26. Gumienna M., Lasik M., Czarnecki Z. 2009. Influence of plant extracts addition on the antioxidative properties of products obtained from green lentil seeds during in vitro d2igestion process. Polish Journal of Food and Nutrition Sciences, 59, 295–298.
• 27. Oboh G. 2006. Antioxidant properties of some commonly consumed and under utilized tropical legumes. Eur Food Res Technol., 224, 61–65.
• 28. E-Siong T., Ah-Heng G., Swan-Choo K. 1995. Carotenoid composition and content of legumes, tubers and starchy roots by HPLC. Mal J Nutr 1: 63-74.
• 29. Fernandez-Marin B., Milla R., Martin-Robles N., Arc E., Kranner I., Becerril J.M., Garcia-Plazaola I. 2014. Side-effects of domestication: cultivated legume seeds contain similar tocopherols and fatty acids but less carotenoids than their wild counterparts. BMC Plant Biology,14:1599.
• 30. Valdivielso I., Bustamante M.A., Gordoa J.C.R., Najera A.I., Renobales M., Barron L.J.R. 2015. Simultaneous analysis of carotenoids and tocopherols in botanical species using one step solid–liquid extraction followed by high performance liquid chromatography. Food Chemistry 173, 709–717.
• 31. Ryan E., Galvin K., O’Connor P., Maguire A.R., O’Brien N.M. 2007. Phytosterol, Squalene, Tocopherol Content and Fatty Acid Profile of Selected Seeds, Grains, and Legumes. Plant Foods Hum Nutr., 62:85–91.
• 32. Wyatt C.J., Carballido S.P., Mendez R.O. 1998. α- and γ-tocopherol Content of Selected Foods in the Mexican Diet: Effect of Cooking Losses. J. Agric. Food Chem. 46, 4657−4661.
• 33. Cho Y.S., Yeum K.J., Chen C.Y., Beretta G., Tang G., Krinsky N.I., Yoon S., Lee‐Kim Y.C., Blumberg J.B., Russell R.M. 2007. Phytonutrients affecting hydrophilic and lipophilic antioxidant activities in fruits, vegetables and legumes. Journal of The Science of Food and Agriculture.Volume 87, Issue6. https://doi.org/10.1002/jsfa.2817.