Yıl 2019, Cilt 2 , Sayı 4, Sayfalar 112 - 116 2019-10-01

Determination of 70 kDa Proteins for Different Temperatures in Cattle Oocytes Matured in vitro

Leyla Bener [1] , Mehmet Kuran [2]


This study was conducted to determine the effect of 36.5 (low) or 38.5 °C (conventional) culture temperatures during in vitro maturation (IVM) on 70 kDa proteins in cattle oocytes. In the study, cattle cumulus oocyte complexes (COC) were subjected to IVM in bicarbonate-buffered TCM–199 supplemented with 10% FCS for 22 hours with a humidified 5% CO2 in air at either 36.5 or 38.5 °C culture temperatures. Maturation of COCs was determined according to fully cumulus expansion at the end of IVM. The cumulus cells of matured COCs were removed by vortexing in TCM-199 with HEPES buffered TCM-199 containing 0.1% hyaluronidase enzyme. Following, denuded oocytes were washed in 9.6 % phosphate buffered saline (PBS) solution for protein isolation. Denuded oocytes (n = 100 for both groups) were centrifuged for 5 min at 300 rpm at 4 °C for PBS removed than the lysis buffer (100 μl) was added and shake 15 min on ice for 30 minutes. For protein isolation, the protein was centrifuged again at 13,000 rpm for 5 minutes and the protein samples were stored at -20 ° C. To increase the concentration of isolated protein samples, the samples were frozen at -80 °C for 24 hours and lyophilized at 0.140 hPa in a lyophilizer. Lyophilized protein samples were stored at -20 °C until SDS-PAGE analysis. The level of 70 kDa proteins in oocytes were determined by SDS-PAGE method. There were no significant differences between low (81.11%) or conventional (84.41%) incubation temperatures in IVM in terms of full cumulus expansion. The 70 kDa proteins band size in SDS-PAGE polyacrylamide gel image of cattle oocytes maturated at low culture temperature was weak compared to cattle oocytes maturated at conventional culture temperature. The results of this study may show that low incubation temperature during IVM decreases the amount of 70 kDa proteins in cattle oocytes.
Oocyte, Maturation, Culture Temperature, 70 kDa Proteins, Cattle
  • Al-Katanani, Y. M., Paula-Lopes, F. F., & Hansen, P. J. (2002). Effect of season and exposure to heat stress on oocyte competence in Holstein cows. Journal of Dairy Science, 85(2), 390-396.Aufricht, C. 2005. Heat–shock protein 70: molecular supertool? Pediatr Nephrol, 20: 707-713.Badinga, L., Thatcher, W. W., Diaz, T., Drost, M., & Wolfenson, D. (1993). Effect of environmental heat stress on follicular development and steroidogenesis in lactating Holstein cows. Theriogenology, 39(4), 797-810.Becker J, Craig, EA. 1994. Heat-shock proteins as molecular chaperones. Eur J Biochem, 219: 11-23.Bernardini C, Fantinati P, Castellani G, Forni M, Zannoni A, Seren E, Bacci ML. 2003. Alteration of constitutive heat sok protein 70 (HSC70) production by in vitro culture of porcine preimplanted embryos. Vet Res Commun, 27(Suppl. 1): 575-578.Camargo LSA, Viana JHM, Sa WF, Ferreira AM, Ramos AA, Vale Filho VR. 2006. Factors influencing in vitro embryo production. Anim. Reprod, 3 (1): 19-28.Cetica PD, Pintos LN, Dalvit GC, Beconi MT. 2001. Antioxidant enzyme activity and oxidative stress in bovine oocyte in vitro maturation. IUBMB Life, 51(1): 57-64.Cevik M, Sen U, Kocyigit A, Soydan E, Kuran M. 2011. Effects of serum, gonadotropins, epidermal growth factor and estradiol 17-beta on cumulus expansion and nuclear maturation of bovine oocytes. Kafkas Univ Vet Fak Derg, 17: 1009-1014.Cevik, M., Kocyigit, A., Sen, U., Kuran, M. 2014. Can commercial human embryo culture media be used in bovine embryo culture? The Journal of the Faculty of Veterinary Medicine, University of Kafkas, 20 (1): 149–153.Edwards JL, Ealy AD, Monterroso VH, Hansen PJ. 1997. Ontogeny of temperature-regulated heat shock protein 70 synthesis in preimplantation bovine embryos. Mol Reprod Dev, 48: 25-33.Edwards JL, Hansen, PJ. 1997). Differential responses of bovine oocytes and preimplantation embryos to heat shock. Mol Reprod Dev, 46: 138-45.Feige U, Morimoto R I, Yahara I, Polla B. 1996. Stress Inducible Cellular Responses, Birkhauser Verlag, 77.Gao J, Zhang W, Dang W, Mou Y, Gao Y, Sun BJ, Du WG. 2014 Heat shock protein expression enhances heat tolerance of reptile embryos. Proc Biol Sci. 281(1791): 1-10.Gilmour DS, Lis JT. 1986. RNA polymerase II interacts with the promoter region of the noninduced hsp70 gene in Drosophila melanogaster cells. Mol Cell Biol, 6: 3984-3989.Hamdoun AM, Cheney DP, Cherr GN. 2003. Phenotypic plasticity of HSP70 and HSP70 gene expression in the Pacific oyster (Crassostrea gigas): implications for thermal limits and induction of thermal tolerance. Biol. Bull. 205, 160– 169.Hendry J, Kola I. 1991. Thermolability of mouse oocytes is due to the lack of expression and/or inducibility of hsp70. Mol Reprod Dev, 28: 1-8.Hunter RHF, Einer-Jensen N, Greve T. 2006 Presence and significance of temperature gradients among different ovarian tissues. Microsc Res Tech, 69: 501-507.Hunter RHF. 2005. The Fallopian tubes in domestic mammals: how vital is their physiological activity? Reprod Nutr Dev, 45: 281-290.Ispada, J., Rodrigues, T. A., Risolia, P. H. B., Lima, R. S., Gonçalves, D. R., Rettori, D., ... & Paula-Lopes, F. F. (2018). Astaxanthin counteracts the effects of heat shock on the maturation of bovine oocytes. Reproduction, Fertility and Development, 30(9), 1169-1179.Ju, J. C., Jiang, S., Tseng, J. K., Parks, J. E., & Yang, X. (2005). Heat shock reduces developmental competence and alters spindle configuration of bovine oocytes. Theriogenology, 64(8), 1677-1689.Karl I, Sorensen JG, Loeschcke V, Fischer K. 2009. HSP70 expression in the copper butterfly Lycaena tityrus across altitudes and temperatures. J. Evol. Biol. 22, 172– 178. Katska-Ksiazkiewicz L, Alm H. 2005. Effect of culture methods on cumulus and oocyte morphology and meiotic competence of bovine oocytes from early antral follicles. Arch Tierz Dummerstorf, 48: 562-571.Kawarsky, S. J., & King, W. A. (2001). Expression and localisation of heat shock protein 70 in cultured bovine oocytes and embryos. Zygote, 9(1), 39-50.Kocyigit, A., Cevik, M., Sen, U., Kuran, M. 2015. The effect of macromolecule and growth factors combinations on in vitro development of bovine embryos. Turkish Journal of Veterinary and Animal Sciences, 39: 308–313.Lánská, V., Chmelíková, E., Sedmíková, M., Petr, J., Rajmon, R., Ješeta, M., & Rozinek, J. (2006). Expression of heat shock protein70 in pig oocytes: Heat shock response during oocyte growth. Animal reproduction science, 96(1-2), 154-164.Leese HJ, Baumann CG, Brison DR, McEvoy TG, Sturmey R.G. 2008. Metabolism of the viable mammalian embryo: quietness revisited. Molec Human Reprod, 14: 667-672.Lenz RW, Ball GD, Leibfried ML, Ax RL, First NL. 1983. İn vitro maturation and fertilization of bovine oocytes are temperature dependent processes. Biol Reprod, 29: 173-179.Maya-Soriano, M. J., Taberner, E., & López-Béjar, M. (2013). Retinol improves in vitro oocyte nuclear maturation under heat stress in heifers. Zygote, 21(4), 377-384.Morimoto RI, Hunt C, Huang S Y, Berg L, Banerji S. 1986. Organization, nucleotide sequence, and transcription of the chicken HSP70 gene. J Biol Chem, 261: 12692-12699.Morimoto RI, Santoro MG. 1998. Stress–inducible responses and heat shock proteins: new pharmacologic targets for cytoprotection. Nat Biotechnol, 16: 833-838.Moulavi F, Hosseini SM, Ashtiani SK, Shahverdi A, Nasr-Esfahani MH. 2006. Can Vero cell co-culture improve in-vitro maturation of bovine oocytes? Reprod Biomed Online, 13: 404-411.Nabenishi, H., Takagi, S., Kamata, H., Nishimoto, T., Morita, T., Ashizawa, K., & Tsuzuki, Y. (2012). The role of mitochondrial transition pores on bovine oocyte competence after heat stress, as determined by effects of cyclosporin A. Molecular reproduction and development, 79(1), 31-40.Parsell DA, Lindquist S. 1993. The function of heatshock proteins in stress tolerance: degradation and reactivation of damaged proteins. Annu Rev Genet, 27: 437-96.Petrof EO, Ciancio M, Chang EB. 2004. Role and regulation of intestinal epithelial heat shock proteins in health and disease. Chin J Dig Dis, 5: 45-50.Pfeifer LFM, Schneider A, Correa MN. 2008. Factors that affect the in vitro production of bovine embryos: A review Rev Colomb Cienc Pecu, 21: 109-120.Ravindranatha BM, Nandi S, Raghu HM, Reddy SM. 2003. İn vitro maturation and fertilization of buffalo oocytes: effects of storage of ovaries, IVM temperatures, storage of processed sperm and fertilization media. Reprod Domest Anim, 38: 21-26.Roth, Z., & Hansen, P. J. (2004). Involvement of apoptosis in disruption of developmental competence of bovine oocytes by heat shock during maturation. Biology of reproduction, 71(6), 1898-1906.Roth, Z., & Hansen, P. J. (2005). Disruption of nuclear maturation and rearrangement of cytoskeletal elements in bovine oocytes exposed to heat shock during maturation. Reproduction, 129(2), 235-244.Sarge KD, Murphy SP, Morimoto RI. 2009. Activation of heat shock gene transcription by Heat Shock Factor 1 involves oligomerization, acquisition of DNA–binding activity, and nuclear localization and can occur in the absence of stress. Mol Cell Biol, 13: 1392-1407.Sen U, Kuran M. 2018. Low incubation temperature successfully supports the in vitro bovine oocyte maturation and subsequent development of embryos. Asian-Australas J Anim Sci, 31(6): 827-834.Sen U. 2015. Effects of sperm from different bulls on developmental competence, blastosist quality and cell number of bovine embryos in vitro. The Journal of the Faculty of Veterinary Medicine, University of Kafkas, 21 (3): 339–344.Shi DS, Avery B, Greve T. 1998. Effects of temperature gradients on in vitro maturation of bovine oocytes. Theriogenology, 50: 667-674.Sørensen JG, Kristensen TN, Loeschcke V. 2003. The evolutionary and ecological role of heat shock proteins. Ecol. Lett. 6, 1025– 1037.Tavares A, Oliveira de D, Felix Lopes RF, Rodrigues LJ. 2005. Gene expression and developmental competence of bovine embryos produced in vitro under varying embryo density conditions. Theriogenology, 64: 1559-1572.Welch RJ, Feramisco, JR. 1984. Nuclear and nucleolar localization of the 72 000-dalton heat shock protein in heatshocked mammalian cells. J Biol Chem, 259: 4501-4513.Ye J, Coleman J, Hunter MG, Craigon J, Campbell KH, Luck MR. 2007. Physiological temperature variants and culture media modify meiotic progression and developmental potential of pig oocytes in vitro. Reprod, 133: 877-886.
Birincil Dil en
Bölüm Makaleler
Yazarlar

Orcid: 0000-0001-6329-5350
Yazar: Leyla Bener (Sorumlu Yazar)
Ülke: Turkey


Orcid: 0000-0002-5139-1721
Yazar: Mehmet Kuran

Tarihler

Yayımlanma Tarihi : 1 Ekim 2019

Bibtex @araştırma makalesi { bsengineering587008, journal = {Black Sea Journal of Engineering and Science}, issn = {}, eissn = {2619-8991}, address = {bsjsci@blackseapublishers.com}, publisher = {Uğur ŞEN}, year = {2019}, volume = {2}, pages = {112 - 116}, doi = {10.34248/bsengineering.587008}, title = {Determination of 70 kDa Proteins for Different Temperatures in Cattle Oocytes Matured in vitro}, key = {cite}, author = {Bener, Leyla and Kuran, Mehmet} }
APA Bener, L , Kuran, M . (2019). Determination of 70 kDa Proteins for Different Temperatures in Cattle Oocytes Matured in vitro. Black Sea Journal of Engineering and Science , 2 (4) , 112-116 . DOI: 10.34248/bsengineering.587008
MLA Bener, L , Kuran, M . "Determination of 70 kDa Proteins for Different Temperatures in Cattle Oocytes Matured in vitro". Black Sea Journal of Engineering and Science 2 (2019 ): 112-116 <https://dergipark.org.tr/tr/pub/bsengineering/issue/47094/587008>
Chicago Bener, L , Kuran, M . "Determination of 70 kDa Proteins for Different Temperatures in Cattle Oocytes Matured in vitro". Black Sea Journal of Engineering and Science 2 (2019 ): 112-116
RIS TY - JOUR T1 - Determination of 70 kDa Proteins for Different Temperatures in Cattle Oocytes Matured in vitro AU - Leyla Bener , Mehmet Kuran Y1 - 2019 PY - 2019 N1 - doi: 10.34248/bsengineering.587008 DO - 10.34248/bsengineering.587008 T2 - Black Sea Journal of Engineering and Science JF - Journal JO - JOR SP - 112 EP - 116 VL - 2 IS - 4 SN - -2619-8991 M3 - doi: 10.34248/bsengineering.587008 UR - https://doi.org/10.34248/bsengineering.587008 Y2 - 2019 ER -
EndNote %0 Black Sea Journal of Engineering and Science Determination of 70 kDa Proteins for Different Temperatures in Cattle Oocytes Matured in vitro %A Leyla Bener , Mehmet Kuran %T Determination of 70 kDa Proteins for Different Temperatures in Cattle Oocytes Matured in vitro %D 2019 %J Black Sea Journal of Engineering and Science %P -2619-8991 %V 2 %N 4 %R doi: 10.34248/bsengineering.587008 %U 10.34248/bsengineering.587008
ISNAD Bener, Leyla , Kuran, Mehmet . "Determination of 70 kDa Proteins for Different Temperatures in Cattle Oocytes Matured in vitro". Black Sea Journal of Engineering and Science 2 / 4 (Ekim 2019): 112-116 . https://doi.org/10.34248/bsengineering.587008
AMA Bener L , Kuran M . Determination of 70 kDa Proteins for Different Temperatures in Cattle Oocytes Matured in vitro. BSJ Eng. Sci.. 2019; 2(4): 112-116.
Vancouver Bener L , Kuran M . Determination of 70 kDa Proteins for Different Temperatures in Cattle Oocytes Matured in vitro. Black Sea Journal of Engineering and Science. 2019; 2(4): 116-112.