The Change in Expression Levels of RYBP and MDM2 Genes in Colorectal Cancer

Öz

Objective: Colorectal cancer, frequently seen among gastrointestinal cancers, is one of the major causes in mortality and morbidity worldwide. In this study, we aimed to investigate the expression levels of RYBP and MDM2 genes in colorectal cancer. We further examined the possible correlation between RYBP and MDM2 expressions in colorectal cancer and the relationship between the expression of these genes and the clinicopathological features of patients with colorectal cancer. Materials and Methods: In this study, fresh tumor colon/rectum tissues and the adjacent healthy tissues collected from 43 patients with colorectal cancer during a surgical operation. RYBP and MDM2 mRNA expressions were examined using Real-Time PCR. Results: In this study results showed that the expression levels of both RYBP and MDM2 were decreased in tumor tissues compared to normal tissues with colorectal cancer patients, but these decreases were not statistically significant (p=0.673 and p=0.721, respectively). Moreover, while there was no correlation between RYBP and MDM2 mRNA expressions in colorectal cancer, no statistically significant relationship was found between the expressions of these genes and clinicopathological data (p>0.05). Conclusion: Consequently, it can be said that RYBP and MDM2 expressions are not related to colorectal cancer, however, future studies with larger sample groups are needed to validate the results obtained from this study.

Anahtar Kelimeler

• Colorectal cancer
• Gene Expression
• RYBP
• MDM2
• Real-Time PCR

Kolorektal Kanserde RYBP ve MDM2 Genlerinin Ekspresyon Düzeylerindeki Değişimi

Öz

Giriş ve Amaç: Gastrointestinal kanserler arasında sıklıkla görülen kolorektal kanser, dünya genelinde mortalite ve morbiditenin en önemli nedenlerinden biridir. Bu çalışmada kolorektal kanserde RYBP ve MDM2 genlerinin ekspresyon düzeylerini araştırmayı amaçladık. Ayrıca kolorektal kanserde RYBP ve MDM2 ekspresyonları arasındaki olası korelasyonu ve bu genlerin ekspresyonu ile kolorektal kanserli hastaların klinikopatolojik özellikleri arasındaki ilişkiyi inceledik. Gereç ve Yöntemler: Bu çalışmada, cerrahi operasyon sonucunda 43 kolorektal kanserli hastanın tümörlü kolon/rektum dokuları ve komşu sağlıklı dokuları toplandı. RYBP ve MDM2 mRNA ekspresyonları Real-Time PCR kullanılarak incelendi. Bulgular: Çalışmanın sonucunda tümörlü dokularda normal dokulara kıyasla hem RYBP hem de MDM2 ekspresyonlarında azalış görüldü fakat bu azalış istatistiksel açıdan anlamlı değildi (sırasıyla p=0.673 ve p=0.721). Ayrıca kolorektal kanserde RYBP ve MDM2 mRNA ekspresyonları arasında korelasyon bulunmazken, bu genlerin ekspresyonları ile klinikopatolojik veriler arasında istatistiksel olarak anlamlı bir ilişki saptanmadı (p>0.05). Sonuç: Sonuç olarak, RYBP ve MDM2 ekspresyonlarının kolorektal kanser ile ilişkili olmadığı söylenebilir, ancak bu çalışmadan elde edilen sonuçları doğrulamak için daha büyük örneklem grupları ile yapılacak çalışmalara ihtiyaç vardır.

Anahtar Kelimeler

• Kolorektal kanser
• Gen ekspresyonu
• RYBP
• MDM2
• Real-Time PCR

Kaynakça

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