Investigation of Neuroprotective Activity of Morus Nigra in Mouse Neuroblastoma Cell Culture Line

Öz

Abstract Aim; Morus Nigra (Moraceae) is a fruit frequently consumed by humans. It is also used in traditional medicine to treat various diseases because it has antiallergic, anti-inflammatory, antibacterial and anticancer properties. It has been stated that Morus Nigra extract has effects on cell viability and some sources do not have this effect. Therefore, this study was carried out to investigate the effects of Morus Nigra extract on neurotoxicity, apoptosis and cell viability in Nb2a cell line Method: Methanol extracts of Morus Nigra were applied to Nb2a cell line at different concentrations (1, 3, 10, 30, 100, 300 and 1000 μg/ml). The effects on the cells were evaluated using neurotoxicity screening test (NTT), apoptosis detection by annexin V method and cell viability/proliferation tests. Results: It was found that methanol extracts of Morus Nigra had no significant effect on cell viability and apoptosis, but showed moderate neuroprotective effect on neurite elongation at concentrations of 100 and 300 µg/ml. Conclusion: These findings indicate that although Morus Nigra is not thought to have anticancer properties, it has been shown that the phenolic compounds it contains may protect axons in the neuronal system due to their antioxidant effects. Keywords: Morus Nigra, Cell culture, Nb2a, apoptosis, NTT

Anahtar Kelimeler

• Morus Nigra
• Cell culture
• Nb2a
• Apoptosis
• NTT

Morus Nigra’nın Fare Nöroblastoma Hücre Kültür Dizinindeki Nöroprotektif Etkinliğinin Araştırılması

Öz

Morus Nigra’nın Fare Nöroblastoma Hücre Kültür Dizinindeki Nöroprotektif Etkinliğinin Araştırılması D. Gülce TATLI1, Kâmil VURAL1Yeşim Turhan ÖZCAN2, Ertan DARIVERENLİ1 1 Manisa Celal Bayar Üniversitesi Farmakoloji /Türkiye 2 İzmir Şehir Hastanesi Çocuk ve Ergen Psikiyatri /Türkiye E-mail: D. Gülce TATLI: [e-mail] Kâmil VURAL: [e-mail] Yeşim Turhan ÖZCAN: [e-mail] Ertan Darıverenli: [e-mail] *Sorumlu Yazar / Corresponding Author: D. Gülce TATLI Gönderim Tarihi / Received: Kabul Tarihi / Accepted: DOI: 10.34087/cbusbed. Öz Giriş ve Amaç: Morus Nigra (Moraceae), insanlar tarafından sıkça tüketilen bir meyvedir. Aynı zamanda geleneksel tıpta çeşitli hastalıkların tedavisinde antialerjik, antienflamatuvar, antibakteriyel ve antikanser özellikleri olduğu için kullanıla gelmektedir. Morus Nigra ektresinin hücre canlılığı üzerine etkileri olduğu bazı kaynaklarda ise bu etkisinin olmadığı belirtilmiştir. Bu nedenle Morus Nigra ektresinin Nb2a hücre hattında nörotoksisite, apoptoz ve hücre canlılığı üzerindeki etkilerini incelemek amacıyla yapılmıştır. Gereç ve Yöntemler: Morus Nigra’nın metanol ekstratları, farklı konsantrasyonlarda (1, 3, 10, 30, 100, 300 ve 1000 μg/ml) Nb2a hücre hattına uygulanmıştır. Nörotoksisite tarama testi (NTT), annexin V yöntemiyle apoptoz tespiti ve hücre canlılık/proliferasyon testleri kullanılarak hücre üzerindeki etkiler değerlendirilmiştir. Bulgular: Morus Nigra'nın metanol ekstratlarının hücre canlılığı ve apoptozis üzerinde belirgin bir etki göstermediğini, ancak nörit uzaması üzerine 100 ve 300 µg/ml konsantrasyonlarda ılımlı nöroprotektif etki gösterdiği ortaya koymuştur. Sonuç: Bu bulgular, Morus Nigra'nın antikanser özellikleri olduğu düşünülmemekle birlikte içerdiği fenolik bileşiklerin antioksidan etkileri nedeniyle nöronal sistemdeki aksonları koruyabileceği gösterilmiştir. Anahtar kelimeler: Morus Nigra, Hücre kültürü, Nb2a, apopitozis, NTT Abstract Aim; Morus Nigra (Moraceae) is a fruit frequently consumed by humans. It is also used in traditional medicine to treat various diseases because it has antiallergic, anti-inflammatory, antibacterial and anticancer properties. It has been stated that Morus Nigra extract has effects on cell viability and some sources do not have this effect. Therefore, this study was carried out to investigate the effects of Morus Nigra extract on neurotoxicity, apoptosis and cell viability in Nb2a cell line Method: Methanol extracts of Morus Nigra were applied to Nb2a cell line at different concentrations (1, 3, 10, 30, 100, 300 and 1000 μg/ml). The effects on the cells were evaluated using neurotoxicity screening test (NTT), apoptosis detection by annexin V method and cell viability/proliferation tests. Results: It was found that methanol extracts of Morus Nigra had no significant effect on cell viability and apoptosis, but showed moderate neuroprotective effect on neurite elongation at concentrations of 100 and 300 µg/ml. Conclusion: These findings indicate that although Morus Nigra is not thought to have anticancer properties, it has been shown that the phenolic compounds it contains may protect axons in the neuronal system due to their antioxidant effects. Keywords: Morus Nigra, Cell culture, Nb2a, apoptosis, NTT

Anahtar Kelimeler

• Morus Nigra
• Hücre kültürü
• Nb2a
• apopitozis
• NTT

Kaynakça

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