The Determination of IL-6 rs1800795 Polymorphism Distribution in Turkish National Cross-Country Skiing Athletes Sub-groups Created Referring to the 1km CCSTAs

Year 2021, Volume: 11 Issue: 4, 782 - 786, 26.10.2021

Derya Kazancı , Tolga Polat , Canan Sercan Doğan , Beste Tacal Aslan , Şehkar Oktay , Muhammed Fatih Bilici , Ömer Kaynar , Başak Funda Eken , Korkut Ulucan

https://doi.org/10.33808/clinexphealthsci.904524

Cited By: 1

Abstract

Objective: Interleukin-6 (IL-6) is an effective and functional protein with a cytokine structure. IL-6 is effective in the immune system like a pro/anti-inflammatory response, and muscle tissue hypertrophy and repair. Our study aimed to investigate the IL-6 rs1800795 polymorphism distribution in Turkish National Cross-Country Skiing Athletes and to determine the advantageous genotype for endurance performance.
Methods: 34 athletes within 3 groups; General (Group-1), Women (Group-2), and Men (Group-3) were enrolled to the study. Also each groups were divided into sub-groups according to their “1-kilometer cross-country average skiing-times (1 km-CCSTAs)”. Blood samples were used for DNA isolation and genotyping were completed by real time- PCR.
Results: The groups' 1 km-CCSTAs were 188.7 ± 22.4, 212.3 ± 17.8, and 177.4 ± 14.1 seconds, respectively. We detected the genotypes as 17 GG (50%) and 17 GC (50%) in Group-1; 5 GG (45%) and 6 GC (55%) in Group-2; 12 GG (52%) and 11 GC (48%) in Group- 3. We detected no CC genotype in groups. Chi-square Fisher’s test was used for statistical analysis.
Conclusion: An increased GC genotype ratio was detected in the faster sub-groups of each group compared to the slower sub-groups. But we obtained the significant genotype distribution only in Group-3 (p <0.0001). Also, an increased C-allele frequency was detected in the faster sub-groups. However, the significant allele gene distribution was obtained only in Group-3 (p=0.0002). According to our results, we can speculate that the GC genotype is more advantageous than the GG genotype in Cross-Country Skiing Athletes.

Keywords

IL-6 genotypes, cross-country skiing, endurance, athletic performance, sports genetics

Supporting Institution

Marmara University Scientific Research Projects Coordination Unit

Project Number

FEN-C-DRP-101018-0535

Thanks

This work has been supported by Marmara University Scientific Research Projects Coordination Unit under grant number

References

• [1] Ulucan K. Spor Genetiği Açısından Türk Sporcuların ACTN3 R577X Polimorfizm Literatür Özeti. Clin Exp Health Sci, 2016; 6: 44-47.
• [2] Cakır E. To investigate the relationship between leg strength and aerobic and anaerobic capacities of cross-country skiers. GUSBD 2019; 4(1): 11-18.
• [3] Ulucan K, Yüksel İ, Sercan C, Kavas NC, Bilici F, Kaynar Ö. Interleukın-6 rs1800795 polymorphısm is not considered as a genetic biomarker in Turkish national skiing running athlete cohort. Fresenius Environmental Bulletin 2020; 29(07A/2020), 6359-6362.
• [4] Eider J, Cieszczyk P, Leonska-Duniec A, Maciejewska A, Sawczuk M, Ficek K, Kotarska K. Association of the 174 G/C polymorphism of the IL6 gene in Polish power-orientated athletes. J Sports Med Phys Fitness. 2013; 53(1): 88-92.
• [5] Eynon N, Ruiz JR, Meckel Y, Santiago C, Fiuza-Luces C, G´omez-Gallego F, Oliveira J, Lucia A. Is the −174 C/G polymorphism of the IL6 gene associated with elite power performance? A replication study with two different Caucasian cohorts. Exp Physiol 2011; 96(2):156–162.
• [6] Ruiz JR, Buxens A, Artied, M, Arteta D, Santiago C, Rodríguez-Romo G, Laoe J, Gallego FG, Lucia A. The −174 G/Cpolymorphism of the IL6 gene is associated with elite power performance. J Sci Med Sport 2010; 13:549–553.
• [7] Arıca S, Solgun HA, Demirel Dengi AS, Bakmaz E, Akçay T, Kapıcı S, Sercan C, Ulucan K. -174 G/C polymorphism of interleukin 6 gene is not significantly different in Turkish professional short and long distance runners. Cell Mol Biol 2018; 64(11):85-87.
• [8] Cenikli A, Nursal AF, Tural E, PolatY, Yalcin M. The Correlation between rs1800795 variant of IL-6 and sports performance among Turkish elite athletes. IJHSSE 2016; 3(11): 1-5.
• [9] Ben-Zaken S, Meckel Y, Nemet D, Kassem E, Eliakim A. Increased prevalence of the IL-6 -174C genetic polymorphism in long distance swimmers. J Hum Kinet 2017; 58:121-130.
• [10] Brandt C, Pedersen BK. The role of exercise-induced myokines in muscle homeostasis and the defense against chronic diseases. J Biomed Biotechnol 2010: 520258.
• [11] Muñoz-Cánoves P, Scheele C, Pedersen BK, Serrano AL. Interleukin-6 myokine signaling in skeletal muscle: a double-edged sword?.FEBS J 2013; 280 (17): 4131–48.
• [12] Nehlson-Cannarella SL, Fagoaga OR, Nieman DC, Henson DA, Butterworth DE, Schmitt RL. Carbohydrate and the cytokine response to 2.5 h of running. J Appl Physiol (1985) 1997; 82(5): 1662-1667.
• [13] Lancaster GI, Khan Q, Drysdale P, Wallace F, Jeukendrup AE, Drayson MT, Gleeson M. The physiological regulation of toll-like receptor expression and function in humans. J Physiol 2005; 563: 945-955.
• [14]Weyestraβ J, Stewart K, Wesselius A, Zeegersb M. Review Ten genetic polymorphisms associated with power athlete status-A meta-analysis. J Sci Med Sport 2018; 21:213-220.