DNA damage and inflammation in COVID-19 cases

Gonca Gülbay; Atakan Savrun

doi:10.17826/cumj.1102599

TR EN

COVID-19 vakalarında DNA hasarı ve enflamasyon

Öz

Amaç: Çalışmamızda, COVID-19 enfeksiyonunu farklı şiddetlerde geçiren hastalarda oksidatif DNA hasarını (8-OHdG), inflamatuar mediatörlerle (IL6 ve TNFA) ilişkisini, ve laboratuvar bulgularına yansımalarını görmeyi amaçladık. Gereç ve Yöntem: Serum interlökin-6 (IL6), tümör nekroz faktör-alfa (TNFA) ve 8-hidroksi-2′-deoksiguanozin (8-OHdG) düzeyleri ELISA (enzyme-linked immunosorbent assay) prensibine dayalı kitler kullanılarak ölçülmüştür. Bulgular: Yoğun bakımda tedavi edilen COVID-19 pozitif hastalarda 8-OHdG marker seviyesi en üst düzeyde ve istatistiksel olarak anlamlı idi. Yatarak tedavi gören hastalarda 8-OHdG marker düzeyi kontrol ve ayakta tedavi gruplarına göre daha yüksekti. Yoğun bakımda tedavi edilen hasta grubunda IL6 değerleri en yüksek düzeyde olup, ayaktan ve kontrol gruplarına göre daha yüksekti. TNFA değerleri açısından kontrol ve hasta grupları arasında istatistiksel olarak anlamlı fark yoktu. Nötrofil-lenfosit oranı (NLR) kontrol grubunda tüm hasta gruplarına göre daha düşüktü. Hastanede yatan hastalarda kontrol grubuna göre C-reaktif protein (CRP) daha yüksekti. Laktat dehidrojenaz (LDH) hastanede yatan hastalarda ayaktan hastalara göre istatistiksel olarak anlamlı derecede yüksek bulundu. Sonuç: COVID-19' un şiddeti arttıkça serum 8-OHdG ve IL6 seviyeleri de artmaktadır. Bu parametreler COVID-19 hastalarının hastalık seyrinin erken evrelerinde tanınmasına rehberlik edebilir.

Anahtar Kelimeler

Covid-19 , DNA damage , Interleukin-6 , Tumor necrosis factor-alpha , 8-hydroxy-2′-deoxyguanosine.

DNA damage and inflammation in COVID-19 cases

Abstract

Purpose: The aim of this study is to see oxidative DNA damage (8-OHdG), its relationship with inflammatory mediators (IL6 and TNFA), and its reflections on laboratory findings in patients who had COVID-19 infection at different intensities. Materials and Methods: Serum interleukin-6 (IL6), tumor necrosis factor-alpha (TNFA), and 8-hydroxy-2′-deoxyguanosine (8-OHdG) levels were measured using kits based on the enzyme-linked immunosorbent assay (ELISA) principle. Results: In COVID-19 positive patients treated in intensive care 8-OHdG marker level is at the highest level and statistically significant. In patients receiving inpatient treatment in the hospitalized, the 8-OHdG marker level is higher than the control and outpatient groups. IL6 values were at the highest level in the patient group treated in the intensive care unit and were higher than the outpatient and control groups. There was no statistically significant difference between the control and patient groups in terms of TNFA values. Neutrophil-to-lymphocyte ratio (NLR) was lower in the control group than in all patient groups. C-reactive protein (CRP) is higher in hospitalized patients than in the control group. Lactate dehydrogenase (LDH) was found to be statistically significantly higher in hospitalized patients than outpatients. Conclusion: As the severity of COVID-19 increases, serum 8-OHdG and IL6 levels also increase. These parameters can guide the diagnosis of COVID-19 patients in the early stages of the disease course.

Keywords

COVID-19 , DNA damage , Interleukin-6 , Tumor necrosis factor-alpha , 8-hydroxy-2′-deoxyguanosine

Kaynakça

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Ayrıntılar

Birincil Dil

İngilizce

Konular

Klinik Tıp Bilimleri

Bölüm

Araştırma Makalesi

Yazarlar

Gonca Gülbay ^*
0000-0001-5201-6352
Türkiye

Atakan Savrun
0000-0001-7468-4159
Türkiye

Yayımlanma Tarihi

30 Eylül 2022

Gönderilme Tarihi

13 Nisan 2022

Kabul Tarihi

17 Temmuz 2022

Yayımlandığı Sayı

Yıl 2022 Cilt: 47 Sayı: 3

DOI

https://doi.org/10.17826/cumj.1102599

IZ

https://izlik.org/JA79JY68NP

MLA

Gülbay, Gonca, ve Atakan Savrun. “DNA damage and inflammation in COVID-19 cases”. Cukurova Medical Journal, c. 47, sy 3, Eylül 2022, ss. 1073-9, doi:10.17826/cumj.1102599.