Antiangiogenic effect of bevacizumab in monoclonal antibodies in experimental inflammatory pain model

Yıl 2019, Cilt: 44 Sayı: Ek 1, 33 - 40, 29.12.2019

Selma Yaman Nursima Çukadar Furkan Baran Fatih Burak Uyanık Metin Kılınç Tufan Mert

https://doi.org/10.17826/cumj.532813

Öz

Purpose: This study aimed to investigate the possible anti-nociceptive, anti-inflammatory and anti-edema effects of systemic (intaperitoneal) and local (intraplantar) bevacizumab in the experimental inflammation model (introduced with Carrageenan). 

Materials and Methods: Sensory functions of rats with inflammatory pain (using Carrageenan (CAR)), performed using healthy and CAR, systemically or locally anti-hypernaphyseceptive effects of bevacizumab at different doses, using thermal plantar test (for hyperalgesia), dynamic plantaraesthesiometer (for allodynia) anti-edema effects were evaluated by measuring paw masses.

Results: The application of local intraplantar (IPL) or intraperitoneal (IP) bevacizumab in healthy rats used as controls in the studies did not cause any change in the thermal latency or mechanical threshold parameters in neurobiophysical tests. CAR application caused a decrease in mechanical threshold and thermal latency parameters and an increase in paw mass. 

Conclusion: Systemic applications of bevacizumab may show more anti-hyperalgesic and anti-allodinic effects. Hyperalgesia pain following CAR application was determined by thermal latency measurements using a stimulus. The resulting hyperalgesia was effectively suppressed by systemic bevacizumab for a long time. 

Anahtar Kelimeler

Anti-hyperalgesic, anti-allodynic, anti-nociceptive, Bevacizumab, pain model

Monoklonal antikorlardan bevacizumab'ın deneysel inflamatuar ağrı modelinde antianjiyogenik etkisi

Öz

Amaç: Bu çalışmada, deneysel inflamatuar modelinde (Carrageenan kullanılarak meydana getirilen) sistemik (intraperitoneal: IP) ve lokal (intraplantar: IPL) olarak uygulanan bevacizumab’ın muhtemel anti-nosiseptif, anti-inflamatuar ve anti-ödem etkilerinin araştırılması amaçlanmıştır.

Gereç ve Yöntem: Sağlıklı ve Carrageenan (CAR) kullanılarak gerçekleştirilen inflamatuar ağrılı sıçanların duysal fonksiyonları termal plantar test (hiperaljezi için), dinamik plantar estesiometre (allodini için) kullanılarak, farklı dozlarda bevacizumab’ın sistemik veya lokal anti-hipernosiseptif etkileri, pençe kütleleri ölçülerek de anti-ödem etkileri değerlendirildi. Biyokimyasal analizler için sıçan pençelerinden alınan örnekler kullanıldı.

Bulgular: Kontrol olarak kullanılan sağlıklı sıçanlarda lokal (intraplantar: IPL) veya sistemik (intraperitoneal: IP) bevacizumab uygulamasının nörobiyofizik testlerde termal latans veya mekanik eşik parametrelerinde herhangi bir değişime neden olmadığı görüldü. CAR uygulaması mekanik eşik ve termal latans parametresinde düşüşlere ve pençe kütlesinde artışa neden oldu. 

Sonuç: Çalışmalarımızda, bevacizumab’ın özellikle sistemik uygulamalarının daha fazla anti-hiperaljezik ve anti-allodinik etkiler gösterebileceği belirlendi. CAR uygulaması sonrası ortaya çıkan hiperaljezi, ağrılı bir uyaranın kullanıldığı termal latans ölçümleriyle belirlendi. Ortaya çıkan hiperaljezi, sistemik bevacizumab tarafından etkili bir şekilde uzun süreli baskılandı. 

Anahtar Kelimeler

Ağrı modeli, anti-hiperaljezik, anti-allodinik, anti-nosiseptif

Kaynakça

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