The effects of alpha-tocopherol on endometrial uterine vascularization, uterine cervix oxidative stress and proliferation in female rats exposed to tobacco smoke

Year 2014, Volume: 39 Issue: 4, 0 - , 22.07.2014

Agnes Erna Taulina Purba Juneris Aritonang Nurdiana Nurdiana Setyawati Soeharto İ Wayan Arsana Wiyasa

https://doi.org/10.17826/cutf.90560

Abstract

Purpose: This study aimed toknow whether administration of -tocopherolable to increase uterine endometrial vascularization, inhibit uterine cervix oxidative stress, and augment cervical proliferation in female rats exposed to tobacco smoke. Material and Methods:Twenty five, 8 weeks old female Wistar rats (Rattusnorvegicus) were divided into 5 groups (n=5 each), including control group (non exposure group); the rats were exposed to tobacco smoke (CS); the rats were exposed to tobacco smoke and treated with -tocopherol at dose of 100 mg/kg/day (CS + AT1); rats were exposed to tobacco smoke and treated with -tocopherol at dose of 200mg/kg/day (CS + AT2); rats were exposed to tobacco smoke and treated with &#945;-tocopherol at dose of 400 mg/kg/day (CS + AT3). The expression of endometrial VEGF was observed by immunohistochemical staining using anti-VEGF primary antibody and biotinylated universal secondary antibody. MDA levels were measured colorimetrically. The number of spiralis arterioles and proliferation of uterine cervix epithelial cells were counted histologically withhematoxilin eosin staining. This research has been approved by research ethics committee Faculty of Medicine University of Brawijaya, Malang, Indonesia (Number 477/EC/KEPK-S2-KB/10/2013). Results: Tobacco smoke significantly lowered VEGF level, the number of spiralis arterioles, anduterine cervix epithelial cells compared with control group (P < 0.05). Administration of -tocopherol in different doses significantly increasethe VEGF level to reachs the level in the control group (P > 0.05). Second and third doses of -tocopherolsignificantly increased the number of spiralis arterioles compared with CS group (P < 0.05), to reached the similar level in the control group (P > 0.05).Exposure to tobacco smoke in rats can increase uterine cervix MDA level significantly compared to the control (P< 0.05). The -tocopherol in various doses cansignificantly reduce MDA level compared to the CS (P 0.05). Conclusion:Administration of -tocopherolcan increase the vascularization in endometrial uterine of rats exposed to tobacco smoke. Besides, -tocopherolinhibit uterine cervix oxidative stress, but can"t induces the proliferation of uterine cervix epithelial cells.

Keywords

lipid soluble antioxidant; oxidative stress, angiogenesis; reproduction.

Sigara Dumanına Maruz Kalan Dişi Sıçanlarda Alfa-Tocopherol"ün Endometrial Uterin Vaskülarizasyonu, Rahim Serviks Oksidatif Stres ve Proliferasyon Üzerine Etkisi

Abstract

Amaç: Bu çalışma -tocopherol uygulamasının uterin endometrium vaskülarizasyonu arttırmada, uterin servik soksidatif stresinin hibe edilmesi ve sigara dumanına maruz kalan dişi sıçanlarda servikal çoğalmanın artırılabileceğinin mümkün olup olmadığını öğrenmeyi amaçlamıştır. Materyal ve Metod: Yirmibeş adet, 8 haftalık dişi Wistar sıçanı (Rattusnorvegicus) kontrol grubu (maruz bırakılmamış grup) dahil 5 gruba (n=5) ayrılmıştır; sigara dumanına (CS) maruz bırakılmış sıçanlar; sigara dumanına maruz bırakılmış ve 100mg/kg/gün dozluk (CS+AT1) -tocopherol"aile muamele edilmiş sıçanlar; sigara dumanına maruz bırakılmış ve 200mg/kg/gündozluk (CS+AT2) -tocopherolile e muamele edilmiş sıçanlar; sigara dumanına maruz bırakılmış ve 400mg/kg/gündozluk (CS+AT3) -tocopherolile e muamele edilmiş sıçanlar. Endometrial VEGF"nin ekspresyonu, anti-VEGF, primer antikor vebiyotinlenmiş genel sekonderantikor kullanılarak immunohistokimyasal boyama ile gözlenmiştir. MDA düzeyleri kolorimetrik olarak ölçülmüştür. Spiral arterlerin sayısı ve Rahim serviks epitel hücrelerin çoğalması hematoksiline ozin boyalarla histolojik olarak sayılmıştır. Bu araştırma Endonezya Malang"daki (No 477/EC/KEPK-S2-KB/10/2013) Brawijaya Üniversitesi Tıp Fakültesi"nin araştırma etik komitesi tarafından onaylanmıştır. Bulgular: Sigara dumanı control grubuyla karşılaştırıldığında özellikle VEGF seviyesini, spiral arterlerin ve uterin serviks epitel hücrelerinin sayısını düşürmüştür (P0.05).a-tocopherol"ün İkinci ve üçüncü dozları CS grubuna kıyasla (P0.05). Sıçanlarda sigara dumanına maruz kalmanın serviks MDA düzeyini control grubuna(P

Keywords

Lipid çözünürantioksidan, oksidatif stres, anjiyogenez, üreme

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• YazışmaAdresi / Address for Correspondence: Dr.Agnes Erna TaulinaPurba Midwifery Master Study Programme, Faculty of Medicine Brawijaya University Jl. Veteran,Malang, East Java, INDONESIA E-mail: [e-mail] G eliştarihi/Received on :10.03.2014
• Kabul tarihi/Accepted on:30.04.2014