Alpha-synuclein levels in multiple sclerosis patients with restless leg syndrome

Yıl 2020, Cilt: 45 Sayı: 2, 562 - 567, 30.06.2020

Suat Çakına Selma Yücel Cemre Çağan Polat Şamil Öztürk

https://doi.org/10.17826/cumj.674564

Öz

Purpose: The restless legs syndrome is more frequent and critical in Multiple Sclerosis patients, and it harms their general well-being and life quality. Alpha-synuclein is a synuclein protein that can have an impact on the pathway of signaling, affecting the Dopamin 2 receptor and its receptor trafficking. Studies have shown that the decrease in dopamine 2 receptor and Restless Legs Syndrome disease are correlated. This study is aimed to ascertain the alpha-synuclein level in multiple sclerosis patients with restless legs syndrome.
Materials and Methods: We took blood samples from 40 multiple sclerosis patients and 20 healthy individuals. Half of the patient group had Multiple Sclerosis with restless leg syndrome. In the study groups, the alpha-synuclein level was determined by quantitative real-time polymerase chain reaction (qRT-PCR) and enzyme-dependent immunosorbent assay (ELISA).
Results: Alpha-synuclein gene expression level was found or be significantly lower in restless leg syndrome patients with multiple sclerosis than the Alpha-synuclein gene expression level in the control group.
Conclusion: Alpha-synuclein may have an impact on the pathogenesis of the restless leg syndrome of multiple sclerosis disease. Further investigations are required to determine the impact of alpha-synuclein in the pathogenesis of restless leg syndrome in multiple sclerosis disease.

Anahtar Kelimeler

Restless leg syndrome, multiple sclerosis, alpha-synuclein, RT-PCR

Destekleyen Kurum

This work was supported by Çanakkale Onsekiz Mart University The Scientific Research Coordination Unit, Project number:

Proje Numarası

TSA-2018-1414

Huzursuz bacak sendromu olan multipl sklerozlu hastalarda alfa-sinüklein düzeyleri

Öz

Amaç: Huzursuz Bacak Sendromu, multipl skleroz hastalığında yaygın olarak görülmektedir, ayrıca hastaların genel sağlığı ve yaşam kalitesi üzerinde olumsuz bir etkisi vardır. Alfa-sinüklein, Dopamin 2 reseptörünün sinyal yolağında önemli bir rol oynamaktadır. Çalışmalar, dopamin 2 reseptörü ve huzursuz bacak sendromu hastalığı arasında ilişki olduğunu göstermiştir. Bu çalışma huzursuz bacak sendromlu multipl skleroz hastalarında alfa-sinükleinin düzeyini belirlemek amacıyla yapılmıştır.

Gereç ve Yöntem: Bu çalışmada 40 multipl skleroz hastasından ve 20 sağlıklı bireyden kan örneği alındı. Hasta grubunun yarısı huzursuz bacak sendromlu multipl skleroz hastalarından oluşmaktadır. Çalışma gruplarında alfa sinüklein düzeyi enzime bağlı immünosorbent kit (ELISA) ve gerçek zamanlı kantitatif polimeraz zincir reaksiyonu (qRT-PCR) metodu kullanılarak belirlendi.

Bulgular: Huzursuz bacak sendromlu multipl skleroz hastalarında alfa-sinüklein gen ekspresyon düzeyi kontrol grubuna göre anlamlı düzeyde düşük bulundu.

Sonuç: Alfa-sinüklein multipl skleroz hastalığında huzursuz bacak sendromu patogenezinde etkili olabilir. Multipl skleroz hastalığında huzursuz bacak sendromu patogenezinde alfa-sinükleinin rolünü belirlemek için daha fazla çalışmaya ihtiyaç vardır.

Anahtar Kelimeler

Huzursuz bacak sendromu, multipl skleroz, alfa-sinüklein, RT-PCR, gen ekspresyonu

Proje Numarası

TSA-2018-1414

Kaynakça

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