Çukurova Bölgesinde akut lenfoblastik lösemili çocuklarda vasküler endotelyal büyüme faktörü (VEGF-C) ve temel fibroblast büyüme faktörü (bFGF) plazma ekspresyonu ve metilasyon seviyeleri

Yıl 2020, Cilt: 45 Sayı: 2, 581 - 587, 30.06.2020

Nihal İnandıklıoglu Osman Demirhan İbrahim Bayram Atila Tanyeli

https://doi.org/10.17826/cumj.676515

Öz

Amaç: Bu çalışmanın amacı akut lenfoblastik lösemili (ALL) çocuklarda vasküler endotel büyüme faktörü-C (VEGF-C) ve temel fibroblast büyüme faktörünün (bFGF) ekspresyon ve metilasyon düzeylerini belirlemekti.
Gereç ve Yöntem: Gerçek zamanlı kantitatif PCR ile yeni tanı almış olan 26 hasta ve 26 sağlıklı kontrolün periferik kan örneklerinde VEGF-C ve bFGF genlerinin mRNA ekspresyon profilleri ve metilasyon seviyelerini tespit ettik.
Bulgular: bFGF ekspresyon seviyesi ALL hastalarında kontrollere kıyasla anlamlı derecede artmıştı (4.23 kat). Ayrıca, VEGF-C ALL hastalarında kontrollere kıyasla anlamlı derecede azalmıştı (3.41 kat). VEGF-C (%6.88) ve bFGF (%16.64) genlerinin promotor bölgelerinin metilasyon oranı ALL hastalarında sağlıklı kontroller göre daha yüksekti.
Sonuç: Hastaların yıllar süren takibi sonucunda VEGF-C ve bFGF'de farklı zaman aralıklarında uzun süreli değişiklikler hastalık riski ile daha güçlü ilişkiler gösterebilir. Hem VEGF-C hem de bFGF ve ALL risk faktörleri ile güçlü bağlantılar kurmak için daha ileri çalışmalara ihtiyaç vardır.

Anahtar Kelimeler

acute lymphoblastic leukemia, basic fibroblast growth factor, gene expression, methylation, vascular endothelial growth factor

Destekleyen Kurum

ÇUKUROVA ÜNİVERSİTESİ

Proje Numarası

TF2013BAP24

Teşekkür

Çukurova Üniversitesi Bilimsel Araştırma Projeleri Birimi projemizi desteklediği için teşekkür ederiz.

Plasma expression and methylation levels of vascular endothelial growth factor (VEGF-C) and basic fibroblast growth factor (bFGF) in children with acute lymphoblastic leukemia in Çukurova Region, Turkey

Öz

Purpose: The aim of this study was to determine the expression and methylation levels of vascular endothelial growth factor-C (VEGF-C) and basic fibroblast growth factor (bFGF) in children with acute lymphoblastic leukemia (ALL).
Materials and Methods: We detected mRNA expression profiles and methylation levels of VEGF-C and bFGF genes in the peripheral blood samples of 26 patients with newly diagnosed ALL and 26 healthy controls by real-time quantitative PCR.
Results: The expression level of bFGF was significantly increased (4.23-fold) in ALL patients as compared with controls. Moreover, VEGF-C were significantly decreased (3.41-fold) in ALL patients as compared with controls. The methylation of the promoter region of VEGF-C (6.88%) and bFGF (16.64%) genes was higher in ALL patients than in healthy controls.
Conclusion: Long-term changes in VEGF-C and bFGF at different time intervals as a result of years of follow-up of patients may show stronger associations with disease risk. Further studies are required to establish strong links with both VEGF-C and bFGF, and ALL risk factors. 

Anahtar Kelimeler

Acute lymphoblastic leukemia, basic fibroblast growth factor

Proje Numarası

TF2013BAP24

Kaynakça

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