Efficacy of pure olive oil and PRF in the prevention of postoperative peritoneal adhesions

Yıl 2022, Cilt: 47 Sayı: 2, 570 - 579, 30.06.2022

Dilan Altıntaş Ural , Duygun Altıntaş Aykan , Muhammed Seyithanoğlu , Abdulkadir Yasir Bahar , Ali Erdal Karakaya , Ahmet Gökhan Güler

https://doi.org/10.17826/cumj.1031388

Cited By: 1

Öz

Purpose: In this study, we applied platelet rich fibrin (PRF) and pure olive oil on the incision surfaces of rats. We aimed to examine whether PRF may be used safely to prevent peritoneal adhesions.
Materials and Methods: Fourty rats were divided into 4 groups (n=8). Eight rats, not included in the study groups, were used to obtain PRF material. Group 1 had no surgical procedure, Group 2 was operated without medication, Group 3 was operated and received 1cc olive oil, Group 4 was operated and received 1 cc PRF. After 21 days, cecum areas were examined histopathologically. Tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), intercellular adhesion molecule-1 (ICAM-1) and platelet-derived growth factor (PDGF) levels were measured in serum by ELISA.
Results: The adhesion scores and severity of fibrosis in Group 3 and 4 were significantly lower than Group 2. Plasma TNF-α value was significantly higher in Group 2 than Group 4. Plasma PDGF value was significantly higher in Group 2 than Group 3 and 4.
Discussion: PRF reduced intestinal adhesion by inhibiting the proliferation of fibroblasts and inflammatory cells, and promoting the proliferation of mesothelial cells. PRF has anti-inflammatory effect and prevented postop adhesions, based mainly on growth factors and cytokines in its content.

Anahtar Kelimeler

postoperatıve perıtoneal adhesıons, platelet rich fibrin, pure olive oil

Teşekkür

Dear Editor-in-Chief, Please find enclosed our manuscript, “Investigation of the Efficacy of Pure Olive Oil and Prf in Preventıng Postoperative Peritoneal Adhesions in Rats" by Dilan Altıntaş Ural et al. which we would like to submit for publication as an original article in Cukurova Medical Journal. We believe this study is interesting to a global audience since it will make contributions to literature and serve well especially to paediatric surgeons. Thus with this features, we hope this article will be found suitable to be published in this precious journal. All of the authors declare that they have all participated in the design, execution, and analysis of the paper, and that they have approved the final version. Additionally, there are no conflicts of interest in connection with this paper and the material described is not under publication or consideration for publication elsewhere. Thank you for your valuable time.Yours sincerely, Corresponding Author: Dr.Dilan Altıntaş URAL, Assistant Professor, Md Mailing Address: Balgat Mahallesi, Mevlana Caddesi, 1422. Sokak, No: 4, 06520, Çankaya/Ankara E-mail: [e-mail] Contact Phone Number :[phone]

Postoperatif peritoneal adhezyonların önlenmesinde saf zeytinyağı ve PRF’nin etkinliği

Öz

Amaç: Bu çalışmada, adhezyon oluşması beklenen alanlara uygulanan trombositten zengin fibrin (PRF) ve saf zeytinyağının etkilerinin incelenmesi amaçlanmıştır. PRF’nin adezyona neden olup olmadığının ve PRF'nin batın içi operasyonlarda güvenle kullanıp kullanamayacağını araştırılmaktadır.
Gereç ve Yöntem: Toplamda 4 grup (n=8) olmak üzere 40 adet Wistar albino rat çalışma gruplarında kullanıldı. Çalışma gruplarına dahil edilmeyen 8 rattan PRF eldesi için yararlanıldı. Grup 1’e herhangi bir cerrahi işlem yapılmadı. Adezyon modelin uygulandığı Grup 2’de ilaç kullanılmadı. Adezyon modeli sonrası Grup 3’de 1 cc zeytinyağı ve Grup 4’de 1 cc PRF uygulandı. 21 gün sonra çekum alanları histopatolojik olarak incelendi. Serumda proinflamatuar sitokinlerden tümör nekroz faktör-α (TNF-α), interlökin-6(IL-6), hücreler arası adezyon molekülü-1(ICAM-1) ve platelet-türevli büyüme faktörü(PDGF) düzeyleri ELISA yöntemiyle ölçüldü.
Bulgular: Grup 3 ve 4'te adezyon skorları ve fibrozis şiddeti Grup 2'ye göre anlamlı olarak düşüktü. Plazma TNF-α değeri Grup 2'de Grup 4'e göre anlamlı olarak yüksekti. Plazma PDGF değeri Grup 2'de Grup 3 ve 4'e göre anlamlı olarak yüksekti. .
Sonuç: PRF, fibroblastların ve inflamatuar hücrelerin çoğalmasını engelleyerek ve mezotelyal hücrelerin çoğalmasını teşvik ederek bağırsak yapışmasını azaltabilir. PRF, içeriğindeki büyüme faktörleri ve sitokinlere bağlı olarak antiinflamatuar etkiye sahiptir ve postop adezyonları önler.

Anahtar Kelimeler

postoperatıve perıtoneal adhesıons, platelet rich fibrin, pure olive oil

Kaynakça

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