Antinociceptive effect of bupropion on visceral pain and its mechanism of action

Yıl 2024, Cilt: 49 Sayı: 2, 415 - 423, 30.06.2024

İsa Yeşilyurt Özge Darakcı Saltık Ayhan Bozkurt

https://doi.org/10.17826/cumj.1429753

Öz

PPurpose: Bupropion is an antidepressant that inhibits noradrenaline and dopamine reuptake. In the current scientific literature, there is limited information regarding the effects of bupropion on pain, predominantly derived from somatic pain studies. This study aims to investigate the impact of bupropion for the first time in visceral pain induced by colorectal distension, a pure visceral pain model, and to reveal the role of various receptors involved in pain control in this effect.
Materials and Methods: Male Sprague-Dawley rats were used in the study. Visceral pain was assessed by colorectal distension-induced visceromotor response. Bupropion is administered gastrically at 5, 10, 20, and 40 mg/kg doses. The mechanism of action of bupropion at the spinal cord level was investigated by intrathecal administration of antagonists of α1 and α2 adrenoceptors (prazosin and yohimbine), D1 and D2 dopamine (SCH 23390 and sulpiride) and opioid receptors (naloxone) 10 minutes before bupropion’s effective dose.
Results: Bupropion showed significant antinociceptive effects at 20 and 40 mg/kg intragastric doses; no difference was found between these two doses. Intrathecally administered yohimbine (30 µg/rats), sulpiride (30 µg/rats, i.t.), and naloxone (2.5 µg/rats) diminished the antinociceptive effect of bupropion. Prazosin and SCH 23390 did not alter bupropion’s effect.
Conclusion: The findings show the antinociceptive effect of bupropion in visceral pain, and adrenergic, dopaminergic, and opioidergic receptors in the spinal cord play a role in this effect.

Anahtar Kelimeler

Antidepressant, Bupropion, visceral, pain, visceromotor

Etik Beyan

This study was approved by Ondokuz Mayis University Animal Experiments Ethics Committee with the acceptance number 2020/25 on 14/07/2020.

Destekleyen Kurum

Ondokuz Mayıs University BAP

Proje Numarası

PYO.TIP.1904.20.012

Bupropionun visseral ağrı üzerindeki antinosiseptif etkisi ve etki mekanizması

Öz

Amaç: Bupropion, noradrenalin ve dopamin geri alımını inhibe eden bir antidepresandır. Bilimsel literatürde, bupropionun ağrı üzerindeki etkilerine ilişkin sınırlı bilgi bulunmaktadır ve bu bilgiler ağırlıklı olarak somatik ağrı çalışmalarından elde edilmiştir. Bu çalışmanın amacı, ilk kez olarak pür viseral ağrı modeli olan kolorektal distansiyon ile indüklenen viseral ağrıda bupropionun etkisini araştırmak ve bu etkide ağrı kontrolünde rol alan çeşitli reseptörlerin rolünü ortaya koymaktır.
Gereç ve Yöntem: Çalışmada erkek Sprague-Dawley sıçanlar kullanıldı. Viseral ağrı, kolorektal distansiyon ile indüklenen viseromotor yanıt ile değerlendirildi. Bupropion 5, 10, 20, 40 mg/kg dozlarında gastrik olarak uygulandı. Bupropionun omurilik seviyesindeki etki mekanizması, α1 ve α2 adrenoseptörlerinin, D1 ve D2 dopamin ve opioid reseptörlerinin antagonistlerinin bupropionun etkili dozundan 10 dakika önce intratekal olarak uygulanmasıyla araştırıldı.
Bulgular: Bupropion 20 ve 40 mg/kg intragastrik dozlarda anlamlı antinosiseptif etki göstermiş, bu iki doz arasında fark bulunmamıştır. İntratekal olarak uygulanan yohimbin (30 µg/sıçan), sülpirid (30 µg/ sıçan, i.t.) ve nalokson (2,5 µg/sıçan) bupropionun antinosiseptif etkisini azaltmıştır. Prazosin ve SCH 23390 bupropionun etkisini değiştirmemiştir.
Sonuç: Bulgular bupropionun visseral ağrıda antinosiseptif etkisini ve bu etkide omurilikteki adrenerjik, dopaminerjik ve opioiderjik reseptörlerin rol oynadığını göstermektedir.

Anahtar Kelimeler

Antidepresan, Bupropion, viiseral, ağrı, viseromotor

Etik Beyan

Bu çalışma Ondokuz Mayıs Üniversitesi Hayvan Deneyleri Etik Kurulu tarafından 14/07/2020 tarihinde 2020/25 kabul numarası ile onaylanmıştır.

Destekleyen Kurum

Ondokuz Mayıs Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi

Proje Numarası

PYO.TIP.1904.20.012

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