Effects of infliximab on testicular torsion/detorsion injury in rats

Year 2024, Volume: 49 Issue: 4, 925 - 934, 30.12.2024

Semiral Albayrak Semalar , Zehra Yılmaz , Günnur Özbakış Dengiz , Kanat Gülle , Meryem Akpolat Ferah , Murat Can , Berrak Guven

https://doi.org/10.17826/cumj.1529709

Abstract

Purpose: This study aimed to investigate whether infliximab (INF), Tumor necrosis factor-alpha (TNF-α) monoclonal antibody, has a protective effect on experimental testiküler torsiyon/detorsiyon (TT/D) injury and whether apoptotic pathways contribute to this possible effect.
Materials and Methods: 42 male Wistar albino rats were randomly divided into three equal main groups: Sham, torsion/detorsion (T/D), and INF+T/D. Each group was then divided into two subgroups with detorsion periods of 24 hours (n =7) and 65 days (n=7). The right testes of anesthetized rats were rotated 720° clockwise for 3 hours to induce torsion. INF (ip, 5 mg/kg) was administered to the rats in the INF+T/D group 10 minutes before detorsion, while saline was administered to the rats in the other groups. At 24 hours after detorsion, the histopathological injury was evaluated by Johnsen scoring and caspase activities by immunohistochemical staining.
Results: Mean testis and cauda epididymis weights, sperm count, and Johnsen score were significantly lower in the T/D group than in the sham group. In addition, marked immunostaining of caspase-3, caspase-8, and caspase-9 was observed in spermatocytes and spermatids in the T/D group. INF administration did not prevent a decrease in testicular (0.80±0.132) and epididymal (0.121±0.247) weights, sperm count (2.0 ± 1.67 x106), or Johnsen score (8.70 ± 0.594). for caspases in spermatogenic cells.
Conclusion: In TT/D injury, INF treatment did not reduce apoptosis and testicular atrophy and did not increase sperm count. TNF-α blockage did not show a protective effect on rat TT/D injury.

Keywords

Caspases, infliximab, sperm, TNF- α, testicular torsion/detorsion injury.

Project Number

BEU BAP-2013-43341027-01

İnfliximabın sıçanlarda testis torsiyon/detorsiyon hasarı üzerindeki etkileri

Abstract

Amaç: Bu çalışmanın amacı, bir TNF-α monoklonal antikoru olan infliksimabın (INF) deneysel testiküler torsiyon/detorsiyon (TT/D) hasarı üzerinde koruyucu etkileri olup olmadığını ve bu olası etkide apoptotik yolakların katkısının olup olmadığını araştırmaktır.
Gereç ve Yöntem: 42 erkek Wistar albino sıçan rastgele eşit üç ana gruba ayrıldı: Sham, torsiyon/detorsiyon(T/D) ve INF+T/D. Her bir grup sonrasında detorsiyon süresi 24 saat (n=7) ve 65 gün (n=7) olan ikişer alt gruba ayrıldı. Anesteziye sıçanların sağ testisleri 3 saat boyunca saat yönünde 720° döndürülerek torsiyon oluşturuldu. INF+T/D grubundaki sıçanlara detorsiyondan 10 dakika önce INF (ip, 5 mg/kg) uygulanırken, diğer gruplardaki sıçanlara salin uygulandı. Detorsiyonun 24. saatinde, histopatolojik hasar Johnsen skorlaması ve kaspaz aktiviteleri immünohistokimyasal boyama ile değerlendirildi.
Bulgular: T/D grubunda ortalama testis ve kauda epididim ağırlıkları, sperm miktarı ve Johnsen skoru sham grubuna göre anlamlı derecede düşüktü. Ek olarak, spermatositlerde ve spermatidlerde kaspaz-3, kaspaz-8 ve kaspaz-9'un belirgin immün boyanması gözlendi. INF, testis (0.80±0.132) ve kauda epididim (0.121±0.247) ağırlıklarındaki, sperm miktarı (2.0 ± 1.67 x106) ve Johnsen skorundaki (8.70 ± 0.594) azalmayı önlemedi.
Sonuç: TT/D hasarında INF tedavisi apoptozu, testiküler atrofiyi azaltmadı ve sperm sayısını artırmadı. Sonuç olarak, TNF-α blokajı sıçan TT/D hasarı üzerinde koruyucu bir etki göstermemiştir.

Keywords

Kaspazlar, infliksimab, sperm, TNF-α, testiküler torsiyon/detorsiyon hasarı.

Project Number

BEU BAP-2013-43341027-01

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