Acinetobacter baumannii’ye spesifik bakteriyofaj araştırılması

Year 2024, Volume: 49 Issue: 4, 1051 - 1056, 30.12.2024

Özgenur Hacıoğlu

https://doi.org/10.17826/cumj.1551746

Abstract

Amaç: Son yıllarda antibiyotiklere olan direncin pek çok önemli bakteri grubunda görülmesiyle, literatüründe faj izolasyon ve karakterizasyon çalışmalarına olan ilginin arttığı ve fajların klinik kullanım potansiyellerinin yaygınlaştığı görülmektedir. Antimikrobiyal direnç profilleri göz önüne alındığında Acinetobacter baumannii enfeksiyonların tedavisinde kullanılacak fajların izolasyonu ve etki spektrumlarının belirlenmesi, karakterizasyonlarının yapılması oldukça önemlidir. Bu çalışmada çevresel su kaynaklarından toplanan su örneklerinden hedef mikroorganizma olan A. baumannii’ye spesifik bakteriyofaj izolasyonu amaçlanmıştır.
Gereç ve Yöntem: Farklı 16 çevresel su örneği faj kaynağı olarak incelenmiştir. Faj konak bakterisi olarak çoklu ilaca dirençli olan A. baumannii klinik izolatları kullanılmıştır. Tek plak izolasyon yöntemiyle izole edilen hedef bakteriye spesifik faj; in vitro çalışmalar süresince çift agar yöntemiyle titresi arttırılarak plak morfolojisi ve konak özgüllüğü değerlendirilmiştir.
Bulgular: vB_KlAcineto13 fajının, yalnızca hedef bakteri üzerinde litik aktivite gösterdiği ve diğer bakteri izolatlarını enfekte etmediği tespit edilmiştir.
Sonuç: Bu çalışmanın bulgularına dayanarak, vB_KlAcineto13 fajının dar bir konak aralığına sahip olduğu ve konak bakterisi dışındaki diğer test edilen bakterileri enfekte etmediği söylenebilir. Ancak, karakterizasyon çalışmalarının faj hakkında daha ayrıntılı bilgi sağlaması muhtemeldir.

Keywords

Bakteriyofaj, faj, faj terapi, Acinetobacter baumannii, faj izolasyon

Project Number

This research was funded by the Kırklareli University Scientific Research Projects Unit (BAP) under project number KLU-BAP-252.

Investigation of Acinetobacter baumannii-specific bacteriophage

Abstract

Purpose: In recent years, the increasing resistance to antibiotics observed in many important bacterial groups has led to a growing interest in the literature regarding phage isolation and characterization, and the expanding clinical potential of phages. Considering the antimicrobial resistance profiles, the isolation of phages to be used in the treatment of Acinetobacter baumannii infections, determination of their action spectrum and characterization are very important. This study aimed to isolate bacteriophages specific to the target microorganism, A. baumannii, from environmental water sources.
Materials and Methods: Sixteen different environmental water samples were investigated as potential sources of phages. Clinical isolates of A. baumannii with multi-drug resistant were used as the host bacteria. Phages specific to the target bacteria were isolated using a single plaque isolation method. During in vitro studies, the titers of the isolated phages were increased using the double agar method, and their plaque morphology and host specificity were evaluated.
Results: The phage vB_KlAcineto13 exhibited lytic activity exclusively against the target bacterium and did not infect other bacterial isolates.
Conclusion: Based on the findings of this study, it can be concluded that phage vB_KlAcineto13 has a narrow host range and does not infect other tested bacteria outside the host bacterium. However, characterization studies are likely to provide more detailed information about the phage.

Keywords

Bacteriophage, phage, phage therapy, Acinetobacter baumannii, phage isolation

Ethical Statement

The author declare no competing interests.

Supporting Institution

This research was funded by the Kırklareli University Scientific Research Projects Unit (BAP) under project number KLU-BAP-252.

Project Number

This research was funded by the Kırklareli University Scientific Research Projects Unit (BAP) under project number KLU-BAP-252.

Thanks

I’m thankful to Professor Suheyla Turkyilmaz and Erbey Cihan, Microbiology Lab, Faculty of Veterinary Medicine, Aydın Adnan Menderes University for providing bacterial strains.

References

• Frieri M, Kumar K, Boutin A. Antibiotic resistance. J. Infect. Public Health. 2017;10:378-69.
• Rice LB. Federal funding for the study of antimicrobial resistance in nosocomial pathogens: no ESKAPE. J. Infect. Dis. 2008;197:1081-79.
• Skurnik M. Can bacteriophages replace antibiotics?. Antibiotics. 2022;11:575.
• Hitchcock NM, Devequi Gomes Nunes D, Shiach J, Valeria Saraiva Hodel K, Dantas Viana Barbosa J, Alencar Pereira Rodrigues L et al. Current clinical landscape and global potential of bacteriophage therapy. Viruses. 2023;15:1020.
• Oduor JM, Onkoba N, Maloba F, Arodi WO, Nyachieo A. Efficacy of lytic Staphylococcus aureus bacteriophage against multidrug-resistant Staphylococcus aureus in mice. J Infect Dev Ctries. 2016;10:1213-08.
• Bao J, Wu N, Zeng Y, Chen L, Li L, Yang L et al. Non-active antibiotic and bacteriophage synergism to successfully treat recurrent urinary tract infection caused by extensively drug-resistant Klebsiella pneumoniae. Emerg Microbes Infect. 2020;9:774-71.
• Li Y, Xiao S, Huang G. Acinetobacter baumannii bacteriophage: progress in isolation, genome sequencing, preclinical research, and clinical application. Curr. Microbiol. 2023;80:199.
• Doughari HJ, Ndakidemi PA, Human IS, Benade S. The ecology, biology and pathogenesis of Acinetobacter spp.: an overview. Microbes Environ. 2011;26:112-101.
• Rao S, Betancourt-Garcia M, Kare-Opaneye YO, Swierczewski BE, Bennett JW, Horne BA et al. Critically ill patient with multidrug-resistant Acinetobacter baumannii respiratory infection successfully treated with intravenous and nebulized bacteriophage therapy. Antimicrob Agents Chemother. 2022;66:e00824-21.
• Badawy S, Pajunen MI, Haiko J, Baka ZA, Abou-Dobara MI, El-Sayed AK et al. Identification and functional analysis of temperate Siphoviridae bacteriophages of Acinetobacter baumannii. Viruses. 2020;12:604.
• Kolsi A, Haukka K, Dougnon V, Agbankpè AJ, Fabiyi K, Virta M et al. Isolation and characterization of three novel Acinetobacter baumannii phages from Beninese hospital wastewater. Arch Virol. 2023;168:228.
• Cihan E, Turkyilmaz S. Investigation of biofilm formation, virulence genes and antibiotic resistance of Acinetobacter baumannii isolates obtained from clinical samples. Isr J Vet Med. 2024;79:2.
• Choi YJ, Kim S, Shin M, Kim J. Isolation and characterization of novel bacteriophages to target carbapenem-resistant Acinetobacter Baumannii. Antibiotics. 2024;13:610.
• Yang H, Liang L, Lin S, Jia S. Isolation and characterization of a virulent bacteriophage AB1 of Acinetobacter baumannii. BMC Microbiol. 2010;10:1.
• Çelik B. Çoğul dirençli Acinetobacter baumannii suşuna özgü litik bakteriyofaj araştırılması (Yüksek lisans tezi). Malatya, İnönü Üniversitesi, 2017.
• Ghajavand H, Esfahani BN, Havaei A, Fazeli H, Jafari R, Moghim S. Isolation of bacteriophages against multidrug resistant Acinetobacter baumannii. Res Pharm Sci.. 2017;12:373-80.
• Erol HB. Hastane enfeksiyonu etkeni Acinetobacter baumannii suşlarına özgü litik bakteriyofajların elde edilmesi ve tedavide kullanım potansiyelinin araştırılması (Doktora tezi). Ankara, Ankara Üniversitesi. 2021.
• Khorshidtalab M, Durukan İ, Tufekci EF, Nas SS, Abdurrahman MA, Kiliç AO. Isolation and characterization of lytic bacteriophages from wastewater with phage therapy potentials against gram-negative bacteria. Eurasian J Med. 2022;54:157.
• Ross A, Ward S, Hyman P. More is better: selecting for broad host range bacteriophages. Front. Microbiol. 2016;7:1352.
• Mendes JJ, Leandro C, Mottola C, Barbosa R, Silva FA, Oliveira M et al. In vitro design of a novel lytic bacteriophage cocktail with therapeutic potential against organisms causing diabetic foot infections. J Med Microbiol. 2014;63:1055-65.
• Popova AV, Zhilenkov EL, Myakinina VP, Krasilnikova VM, Volozhantsev NV. Isolation and characterization of wide host range lytic bacteriophage AP22 infecting Acinetobacter baumannii. FEMS. Microbiol. Lett. 2012;332:40-6.