Araştırma Makalesi
BibTex RIS Kaynak Göster

Proliferation of Mesenchymal Stem Cells in Carbonate Apatite-Chitosan Scaffolds in Bone Tissue Engineering Techniques

Yıl 2019, , 412 - 418, 29.12.2019
https://doi.org/10.7126/cumudj.595455

Öz

Objectives: Chitosan (poly-1,4-D-glucosamine) become popular
as bone graft material. It has a high biocompatibility and biodegradability,
non-toxic, bacteriostatic, and has good adsorption properties. It also enhances
the proliferation and differentiation of the osteoblast cell cultures. However,
chitosan also has a weakness for cell adhesion and the lack of sufficient bone
formation capabilities. To improve it, we tried to develop a chitosan scaffolds
that combine with carbonate apatite (CA), which has excellent biocompatibility
properties of the tissues of the human body. 
Purpose:
to make the carbonate apatite-chitosan scaffolds (CA-ChSs) and evaluate CA-ChSs
from the standpoint of cell proliferation using mesenchymal stem cells (MSCs). 

Materials and Methods: Porous chitosan matrix was made by using the
lyophilization technique. ChSs containing chitosan powder was made by the
following procedure. 100, 150, 200, and 400 mg of chitosan powder was dissolved
in 5 ml of 2% acetic acid at room temperature. To make CA-ChSs, ChSs containing
200 mg of chitosan powder was chosen. Ultraviolet radiation was then performed
for 2 hours. CA-ChSs structure was observed by a scanning electron microscope
(SEM). Proliferation of MSCs in CA-ChSs evaluated at days 1, 3, 5 and 7.
Results:This study demonstrated that CA-ChSs containing
200 mg of chitosan powder and 50 mg CA has a three-dimensional structure that
is porous and attachment powder CA in the pores and absorbance values were
increased from the examination day 1 until day 7. 
Conclusion:Based on these results, it is expected CA-ChSs
may be candidates for bone graft material in tissue engineering techniques.

Kaynakça

  • Debnath, T. Ghosh, S. Potlapuvu, US. Kona, L, Kamaraju, SR. Sarkar, S. Gaddam, S. Chelluri, LK. Proliferation and differentiation potential of human adipose-derived stem cells grown on cytosan hydrogel. Plos One. 2015; 10(3): e0120803.
Yıl 2019, , 412 - 418, 29.12.2019
https://doi.org/10.7126/cumudj.595455

Öz

Kaynakça

  • Debnath, T. Ghosh, S. Potlapuvu, US. Kona, L, Kamaraju, SR. Sarkar, S. Gaddam, S. Chelluri, LK. Proliferation and differentiation potential of human adipose-derived stem cells grown on cytosan hydrogel. Plos One. 2015; 10(3): e0120803.
Toplam 1 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Sağlık Kurumları Yönetimi
Bölüm Original Research Articles
Yazarlar

Aqsa Sjuhada Oki 0000-0003-4427-2561

Maretaningtias Dwi Ariani 0000-0002-7473-2015

Yayımlanma Tarihi 29 Aralık 2019
Gönderilme Tarihi 23 Temmuz 2019
Yayımlandığı Sayı Yıl 2019

Kaynak Göster

EndNote Oki AS, Ariani MD (01 Aralık 2019) Proliferation of Mesenchymal Stem Cells in Carbonate Apatite-Chitosan Scaffolds in Bone Tissue Engineering Techniques. Cumhuriyet Dental Journal 22 4 412–418.

Cumhuriyet Dental Journal (Cumhuriyet Dent J, CDJ) is the official publication of Cumhuriyet University Faculty of Dentistry. CDJ is an international journal dedicated to the latest advancement of dentistry. The aim of this journal is to provide a platform for scientists and academicians all over the world to promote, share, and discuss various new issues and developments in different areas of dentistry. First issue of the Journal of Cumhuriyet University Faculty of Dentistry was published in 1998. In 2010, journal's name was changed as Cumhuriyet Dental Journal. Journal’s publication language is English.


CDJ accepts articles in English. Submitting a paper to CDJ is free of charges. In addition, CDJ has not have article processing charges.

Frequency: Four times a year (March, June, September, and December)

IMPORTANT NOTICE

All users of Cumhuriyet Dental Journal should visit to their user's home page through the "https://dergipark.org.tr/tr/user" " or "https://dergipark.org.tr/en/user" links to update their incomplete information shown in blue or yellow warnings and update their e-mail addresses and information to the DergiPark system. Otherwise, the e-mails from the journal will not be seen or fall into the SPAM folder. Please fill in all missing part in the relevant field.

Please visit journal's AUTHOR GUIDELINE to see revised policy and submission rules to be held since 2020.