Determination of the Effective Time of Denture Cleanser Tablets on the Removal of Candida Albicans on Denture Base Resins

Yıl 2019, Cilt: 22 Sayı: 3, 325 - 335, 09.09.2019

Yeliz Hayran

https://doi.org/10.7126/cumudj.566223

Öz

Objective: The aim of this study was to determinate of the
effective time of different denture cleanser tablets on the removal of Candida albicans on various base resins.

Materials
and Methods: Conventional
heat cured resin, high impact resin, autopolymerized resin, and polyamide
resin were used. 160 samples were prepared for each resin type.  The biofilm of C. albicans was formed on the resins and then exposed to alkaline
peroxide tablet and enzymatic tablets for 3-5-10-20-40-80-160-200 minutes. Cell
viability was assessed by MTT test. 3-way ANOVA was used for statistical
analysis.

Results: The
effect of resin type, tablet type and application time on cell viability of C. albicans were found to be significant
(p<0.05). Cell death was at least
65% on the resins even at the minimum time. Furthermore, as the duration of
administration of both tablets increased, cell viability in all resins tended
to decrease. The administration of both tablets on all resins for 10 min
resulted in approximately 80% cell death. Additionally, the most significant
antimicrobial activities of the tablets were determined on 20^th
minutes. The alkaline peroxide tablet on all base resins for application
periods of 3 to 200 minutes was generally more effective than the enzymatic
tablet.

Conclusion:
Alkaline peroxide and enzymatic cleanser tablets showed remarkable anticandidal
activity for all resins. The patients with risk of C. albicans infection should keep their prostheses in cleanser
tablets for at least 20 minutes. Furthermore, the anticandidal effect tended to
increase with prolonged exposure time. 

Anahtar Kelimeler

Denture bases, Candida albicans, Cell survival, Denture Cleansers

Protez kaide rezinleri üzerindeki Candida albicans'ın uzaklaştırılmasında protez temizleyici tabletlerin etkin sürelerinin belirlenmesi

Öz

Amaç:
Bu çalışmanın amacı, çeşitli protez kaide rezinleri üzerindeki Candida albicans'ın uzaklaştırılmasında farklı
protez temizleyici tabletlerin etkin sürelerinin belirlenmesidir.

Gereç ve Yöntemler: Çalışmada geleneksel ısıyla polimerize olan rezin, yüksek çarpma
dayanıklı rezin, otopolimerize rezin ve poliamid rezin kullanıldı. Her rezin
tipi için 160 numune hazırlandı. C.
albicans'ın biyofilmi rezinler üzerinde oluşturuldu ve daha sonra
3-5-10-20-40-80-160-200 dakika boyunca alkalin peroksit tablet ve enzimatik
tablete maruz bırakıldı. Hücre canlılığı MTT testi ile değerlendirildi.
İstatistiksel analiz için 3 yönlü ANOVA kullanıldı.

Bulgular:
Rezin tipi, tablet tipi ve uygulama süresinin rezinler üzerindeki C. albicans'ın hücre canlılığı üzerine
etkisi anlamlı bulundu (p<0.05). Minimum
sürede bile rezinlerde en az % 65 hücre ölümü meydana geldi. Bununla birlikte,
her iki tabletin uygulama süresi arttıkça, tüm rezinlerdeki hücre canlılığı azalma
eğilimi gösterdi. Rezinlerin her iki tablete de 10 dakika maruz bırakılması tüm
rezinlerde yaklaşık % 80 hücre ölümüne neden oldu. Bununla birlikte,
tabletlerin en önemli antimikrobiyal aktiviteleri 20. dakika da görülmüştür. Alkalin
peroksit tablet, tüm protez kaide rezinleri üzerinde 3 ila 200 dakikalık uygulama
süreleri için genel olarak enzimatik tabletten daha etkili olmuştur.

Sonuç:
Alkalin peroksit ve enzimatik temizleyici tabletler, tüm rezinler için kayda
değer bir antikandidal aktivite göstermiştir. C. albicans enfeksiyonu riski taşıyan hastalar protezlerini temizleyici
tabletlerde en az 20 dakika tutmalıdır. Bununla birlikte, antikandidal etki tablet
süresinin uzaması ile artma eğilimindedir.

Anahtar Kelimeler

Protez kaideleri, Kandida albicans, Hücre canlılığı, Protez temizleyicilerİ

Kaynakça

• Jagger DC, Harrison A, Jandt KD. The reinforcement of dentures. J Oral Rehabil 1999;26:185-94.
• Rodford R. The development of high impact strength denture-base materials. J Dent 1986;14:214-7.
• Yunus N, Rashid A, Azmi L, Abu‐Hassan M. Some flexural properties of a nylon denture base polymer. J Oral Rehabil 2005;32:65-71.
• Costerton JW, Stewart PS, Greenberg EP. Bacterial biofilms: a common cause of persistent infections. Science 1999;284:1318-22.
• Barbeau J, Seguin J, Goulet JP, de Koninck L, Avon SL, Lalonde B et al. Reassessing the presence of Candida albicans in denture-related stomatitis. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2003;95:51-9.
• Kulak-Ozkan Y, Kazazoglu E, Arikan A. Oral hygiene habits, denture cleanliness, presence of yeasts and stomatitis in elderly people. J Oral Rehabil 2002;29:300-4.
• Lucena-Ferreira SC, Ricomini-Filho AP, Silva WJ, Cury JA, Cury AA. Influence of daily immersion in denture cleanser on multispecies biofilm. Clin Oral Investig 2014;18:2179-85.
• Freitas-Fernandes FS, Cavalcanti YW, Ricomini Filho AP, Silva WJ, Del Bel Cury AA, Bertolini MM. Effect of daily use of an enzymatic denture cleanser on Candida albicans biofilms formed on polyamide and poly(methyl methacrylate) resins: an in vitro study. J Prosthet Dent 2014;112:1349-55.
• Ferreira MA, Pereira-Cenci T, Rodrigues de Vasconcelos LM, Rodrigues-Garcia RC, Del Bel Cury AA. Efficacy of denture cleansers on denture liners contaminated with Candida species. Clin Oral Investig 2009;13:237-42.
• Hayran Y, Sarikaya I, Aydin A, Tekin YH. Determination of the effective anticandidal concentration of denture cleanser tablets on some denture base resins. J Appl Oral Sci 2018;26:e20170077.
• Jagger DC, Al-Akhazam L, Harrison A, Rees JS. The effectiveness of seven denture cleansers on tea stain removal from PMMA acrylic resin. Int J Prosthodont 2002;15:549-52.
• Nikawa H, Hamada T, Yamamoto T. Denture plaque--past and recent concerns. J Dent 1998;26:299-304.
• Kuramitsu HK, He X, Lux R, Anderson MH, Shi W. Interspecies interactions within oral microbial communities. Microbiol Mol Biol Rev 2007;71:653-70.
• Klotz SA, Drutz DJ, Zajic JE. Factors governing adherence of Candida species to plastic surfaces. Infec Immun 1985;50:97-101.
• de Souza RF, de Freitas Oliveira Paranhos H, Lovato da Silva CH, Abu-Naba'a L, Fedorowicz Z, Gurgan CA. Interventions for cleaning dentures in adults. Cochrane Database Syst Rev 2009:CD007395.
• Karakis D, Akay C, Oncul B, Rad AY, Dogan A. Effectiveness of disinfectants on the adherence of Candida albicans to denture base resins with different surface textures. J Oral Sci 2016;58:431-7.
• Dolan TA, Gilbert GH, Duncan RP, Foerster U. Risk indicators of edentulism, partial tooth loss and prosthetic status among black and white middle-aged and older adults. Community Dent Oral Epidemiol 2001;29:329-40.
• Ozkan YK, Ucankale M, Ozcan M, Uner N. Effect of denture adhesive on the micro-organisms in vivo. Gerodontology 2012;29:9-16.
• Nikawa H, Hamada T, Yamashiro H, Kumagai H. A review of in vitro and in vivo methods to evaluate the efficacy of denture cleansers. Int J Prosthodont 1999;12:153-9.
• de Freitas Fernandes FS, Pereira-Cenci T, da Silva WJ, Filho AP, Straioto FG, Del Bel Cury AA. Efficacy of denture cleansers on Candida spp. biofilm formed on polyamide and polymethyl methacrylate resins. J Prosthet Dent 2011;105:51-8.
• Berger JC, Driscoll CF, Romberg E, Luo Q, Thompson G. Surface roughness of denture base acrylic resins after processing and after polishing. J Prosthodont.2006;15:180-6.
• Radford DR, Sweet SP, Challacombe SJ, Walter JD. Adherence of Candida albicans to denture-base materials with different surface finishes. J Dent 1998;26:577-83.
• Kawara M, Iwata Y, Iwasaki M, Komoda Y, Iida T, Asano T et al. Scratch test of thermoplastic denture base resins for non-metal clasp dentures. J Prosthodont Res 2014;58:35-40.
• da Silva FC, Kimpara ET, Mancini MN, Balducci I, Jorge AO, Koga-Ito CY. Effectiveness of six different disinfectants on removing five microbial species and effects on the topographic characteristics of acrylic resin. J Prosthodont 2008;17:627-33.
• Sesma N, Lagana DC, Morimoto S, Gil C. Effect of denture surface glazing on denture plaque formation. Braz Dent J 2005;16:129-34.
• Radford DR, Challacombe SJ, Walter JD. Denture plaque and adherence of Candida albicans to denture-base materials in vivo and in vitro. Crit Rev Oral Biol Med 1999;10:99-116.
• Pereira-Cenci T, Cury AA, Cenci MS, Rodrigues-Garcia RC. In vitro Candida colonization on acrylic resins and denture liners: influence of surface free energy, roughness, saliva, and adhering bacteria. Int J Prosthodont 2007;20:308-10.
• Nevzatoglu EU, Ozcan M, Kulak-Ozkan Y, Kadir T. Adherence of Candida albicans to denture base acrylics and silicone-based resilient liner materials with different surface finishes. Clin Oral Investig 2007;11:231-6.
• Handa RK, Jagger DC, Vowles RW. Denture cleansers, soft lining materials and water temperature: what is the effect? Prim Dent Care 2008;15:53-8.
• Nikawa H, Jin C, Makihira S, Egusa H, Hamada T, Kumagai H. Biofilm formation of Candida albicans on the surfaces of deteriorated soft denture lining materials caused by denture cleansers in vitro. J Oral Rehabil 2003;30:243-50.
• Fernandes CB, Aquino DR, Franco GC, Cortelli SC, Costa FO, Cortelli JR. Do elderly edentulous patients with a history of periodontitis harbor periodontal pathogens? Clin Oral Implants Res 2010;21:618-23.
• Mukherjee PK, Chandra J. Candida biofilm resistance. Drug Resist Updat 2004;7:301-9.
• Ramage G, Saville SP, Wickes BL, Lopez-Ribot JL. Inhibition of Candida albicans biofilm formation by farnesol, a quorum-sensing molecule. Appl Environ Microbiol 2002;68:5459-63.
• Hahnel S, Rosentritt M, Burgers R, Handel G, Lang R. Candida albicans biofilm formation on soft denture liners and efficacy of cleaning protocols. Gerodontology 2012;29:e383-91.