Yıl 2019, Cilt 22 , Sayı 3, Sayfalar 325 - 335 2019-09-09

Determination of the Effective Time of Denture Cleanser Tablets on the Removal of Candida Albicans on Denture Base Resins
Protez kaide rezinleri üzerindeki Candida albicans'ın uzaklaştırılmasında protez temizleyici tabletlerin etkin sürelerinin belirlenmesi

Yeliz HAYRAN [1]


Objective: The aim of this study was to determinate of the effective time of different denture cleanser tablets on the removal of Candida albicans on various base resins.

Materials and Methods: Conventional heat cured resin, high impact resin, autopolymerized resin, and polyamide resin were used. 160 samples were prepared for each resin type.  The biofilm of C. albicans was formed on the resins and then exposed to alkaline peroxide tablet and enzymatic tablets for 3-5-10-20-40-80-160-200 minutes. Cell viability was assessed by MTT test. 3-way ANOVA was used for statistical analysis.

Results: The effect of resin type, tablet type and application time on cell viability of C. albicans were found to be significant (p<0.05). Cell death was at least 65% on the resins even at the minimum time. Furthermore, as the duration of administration of both tablets increased, cell viability in all resins tended to decrease. The administration of both tablets on all resins for 10 min resulted in approximately 80% cell death. Additionally, the most significant antimicrobial activities of the tablets were determined on 20th minutes. The alkaline peroxide tablet on all base resins for application periods of 3 to 200 minutes was generally more effective than the enzymatic tablet.

Conclusion: Alkaline peroxide and enzymatic cleanser tablets showed remarkable anticandidal activity for all resins. The patients with risk of C. albicans infection should keep their prostheses in cleanser tablets for at least 20 minutes. Furthermore, the anticandidal effect tended to increase with prolonged exposure time. 


Amaç: Bu çalışmanın amacı, çeşitli protez kaide rezinleri üzerindeki Candida albicans'ın uzaklaştırılmasında farklı protez temizleyici tabletlerin etkin sürelerinin belirlenmesidir.

Gereç ve Yöntemler: Çalışmada geleneksel ısıyla polimerize olan rezin, yüksek çarpma dayanıklı rezin, otopolimerize rezin ve poliamid rezin kullanıldı. Her rezin tipi için 160 numune hazırlandı. C. albicans'ın biyofilmi rezinler üzerinde oluşturuldu ve daha sonra 3-5-10-20-40-80-160-200 dakika boyunca alkalin peroksit tablet ve enzimatik tablete maruz bırakıldı. Hücre canlılığı MTT testi ile değerlendirildi. İstatistiksel analiz için 3 yönlü ANOVA kullanıldı.

Bulgular: Rezin tipi, tablet tipi ve uygulama süresinin rezinler üzerindeki C. albicans'ın hücre canlılığı üzerine etkisi anlamlı bulundu (p<0.05). Minimum sürede bile rezinlerde en az % 65 hücre ölümü meydana geldi. Bununla birlikte, her iki tabletin uygulama süresi arttıkça, tüm rezinlerdeki hücre canlılığı azalma eğilimi gösterdi. Rezinlerin her iki tablete de 10 dakika maruz bırakılması tüm rezinlerde yaklaşık % 80 hücre ölümüne neden oldu. Bununla birlikte, tabletlerin en önemli antimikrobiyal aktiviteleri 20. dakika da görülmüştür. Alkalin peroksit tablet, tüm protez kaide rezinleri üzerinde 3 ila 200 dakikalık uygulama süreleri için genel olarak enzimatik tabletten daha etkili olmuştur.

Sonuç: Alkalin peroksit ve enzimatik temizleyici tabletler, tüm rezinler için kayda değer bir antikandidal aktivite göstermiştir. C. albicans enfeksiyonu riski taşıyan hastalar protezlerini temizleyici tabletlerde en az 20 dakika tutmalıdır. Bununla birlikte, antikandidal etki tablet süresinin uzaması ile artma eğilimindedir.

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Birincil Dil en
Konular Sağlık Bilimleri ve Hizmetleri
Bölüm Original Research Articles
Yazarlar

Orcid: 0000-0002-8664-9083
Yazar: Yeliz HAYRAN (Sorumlu Yazar)
Kurum: TOKAT GAZİOSMANPAŞA ÜNİVERSİTESİ, DİŞ HEKİMLİĞİ FAKÜLTESİ
Ülke: Turkey


Tarihler

Başvuru Tarihi : 16 Mayıs 2019
Kabul Tarihi : 9 Ağustos 2019
Yayımlanma Tarihi : 9 Eylül 2019

EndNote %0 Cumhuriyet Dental Journal Determination of the Effective Time of Denture Cleanser Tablets on the Removal of Candida Albicans on Denture Base Resins %A Yeliz Hayran %T Determination of the Effective Time of Denture Cleanser Tablets on the Removal of Candida Albicans on Denture Base Resins %D 2019 %J Cumhuriyet Dental Journal %P 1302-5805-2146-2852 %V 22 %N 3 %R doi: 10.7126/cumudj.566223 %U 10.7126/cumudj.566223