Cytotoxic effect of Zataria multiflora on breast cancer cell line (MCF-7) and normal fibroblast cells

Yıl 2015, Cilt: 36 Sayı: 3, 1895 - 1904, 13.05.2015

Farkhondeh Nemati Maryam Janitermi

Öz

Abstract. The aim of this study was to evaluate the cytotoxic effects of Zataria multifloraessential oilon the human breast cancer cell line (MCF-7) and fibroblast cells. The essential oil of Z. multiflorawas obtained by distillation. Cells were cultured with various concentrations of Z. multiflora essential oil(6.25, 12.5, 25, 50, 100, 200 and 400 µg/ml) for 24, 48 and 72 hours by MTT testAfter 24, 48 and 72 h results revealed that the more effect of Z. multifloraessenceon the growth inhibitory of MCF-7 cell line was observed at 200 μg/ml (102.01, 105.17 and 99.96% respectively). These effects became more prominent after 48 hours. The IC50 values after 24, 48 and 72 h were 158.97, 30.44 and 36.63 mg/ml respectively. Also results showed that more effect of Z. multifloraessenceon the growth inhibitory of normal fibroblast cell was observed at 100 μg/ml after 48h (98.82%).

Anahtar Kelimeler

Zataria multiflora, cytotoxic effect, MCF-7 cells, fibroblast cells, MTT assay

Kaynakça

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