Chemical Composition of Lamium purpureum L. and Determination of Anticancer Activity of Its Essential Oil on Melanoma

Abstract

The current study aimed to evaluate Lamium purpureum L. in terms of its chemical composition and the potential of its essential oil for anticancer activity. The profiling analysis of its chemical composition was carried out via GC-MS. For this purpose, the ethanol extract was subjected to GC-MS analysis to determine chemical compounds. To obtain essential oil, steam distillation of the whole plant (aerial, leaf, stem) was carried out in a Clevenger apparatus. The anticancer activitiy was determined via MTT assay. We observed that the maximum cell death was 14% at 50 μg/mL concentration. As a result of GC-MS analysis, palmitic acid, 7-tetradecenal, octadecadienoic acid, acetic acid, ethyl 9,12,15-octadecatrienoate, hexatriacontane, stearate and benzyl benzoate were identified as major components. We determined that the essential oil of L. purpureum L. had essential compounds which showed cytotoxic activities.

Keywords

• GC-MS analysis,Anticancer activity,MTT,Essential oil,Lamium purpureum L.

Lamium purpureum L.’nin Kimyasal Bileşimi ve Uçucu Yağının İnsan Melanom Kanseri Üzerine Antikanser Aktivitesinin Belirlenmesi

Abstract

Bu çalışma Lamium purpureum L.'yi kimyasal bileşimi ve antikanser aktivitesi için uçucu yağ potansiyeli açısından değerlendirmeyi amaçlamıştır. Kimyasal bileşiminin profil analizi GC-MS ile yapılmıştır. Bu amaçla, etanol ekstresi kimyasal bileşiklerin belirlenmesi için GC-MS analizine tabi tutuldu. Esansiyel yağ elde etmek için, tüm bitkinin (hava, yaprak, gövde) buharla damıtılması bir Clevenger aparatında gerçekleştirildi. Antikanser aktivitesi MTT testi ile belirlendi. 50 μg/mL konsantrasyonunda maksimum hücre ölümünün% 14 olduğunu gözlemledik. GC-MS analizi sonucunda palmitik asit, 7-tetradekenal, oktadecadienoik asit, asetik asit, etil 9,12,15-oktadekatrienoat, heksatriakontan, stearat ve benzil benzoat ana bileşenler olarak tanımlandı. L. purpureum L.'ın uçucu yağının sitotoksik aktivite gösteren temel bileşiklere sahip olduğunu belirledik.

Keywords

• GC-MS analizi,Antikanser aktivite,MTT,Esansiyel yağ,Lamium purpureum L.

References

1. [1] A.B. da Rocha, R.M. Lopes and G. Schwartsmann, “Natural products in anticancer therapy", Current Opinion in Pharmacology, vol. 1, no. 4, pp. 364-369, 2001.
2. [2] N. Saraç, A. Uğur and M.E. Duru, “Antimicrobial activity and chemical composition of the essential oils of Thymbra spicata var. intricata”, International Journal of Green Pharmacy, vol. 3, no. 1, pp. 24-28, 2009.
3. [3] R. Paduch, G. Matysik, M. Wójciak–Kosior, M. Kandefer–Szerszeń, A. Skalska–Kamińska, M. Nowak–Kryska, P. Niedziela. “Lamium Album Extracts Express Free Radical Scavenging and Cytotoxic Activities”, Polish Journal of Environmental Studies, vol. 17, no. 4, pp. 569-580, 2008.
4. [4] F.N. Yalçın and D. Kaya, "Ethnobotany, pharmacology and phytochemistry of the genus Lamium (Lamiaceae).", FABAD Journal of Pharmaceutical Sciences, vol. 31, no.1, pp. 43-52, 2006.
5. [5] F. Celep and T. Dirmenci. "Systematic and Biogeographic overview of Lamiaceae in Turkey." Natural Volatiles & Essential Oils, vol. 4, no. 4, pp. 14-27, 2017.
6. [6] Y.Z. Kocabaş and Ş. Karaman. "Essential oils of Lamiaceae family from south east Mediterranean region (Turkey)." Pakistan Journal of Biological Sciences, vol. 4, no. 10, pp. 1221-1223, 2001.
7. [7] M. Heinrich, J. Barnes, S. Gibbons and E.M.Williamson. Fundamentals of Pharmacognosy and Phytotherapy, Second edition, Churchill Livingstone, London, 2012, pp. 40-41.
8. [8] G. Nieto, "Biological activities of three essential oils of the Lamiaceae family." Medicines, vol. 4, no. 3, p. 63, 2017.

9. [9] F. Naghibi, M. Mosaddegh, S.M. Motamed, A. Ghorbani "Labiatae family in folk medicine in Iran: from ethnobotany to pharmacology." Iranian Journal of Pharmaceutical Research, vol. 4, no. 2, pp. 63-79, 2005.
10. [10] F.N. Yalçın, D. Kaya, E. Kılıç, M. Özalp, T. Ersöz, İ. Çalış. “Antimicrobial and free radical scavenging activities of some Lamium species from Turkey.” Hacettepe University Journal of the Faculty of Pharmacy, vol. 27, no. 1, pp. 11-22, 2007.
11. [11] K. Canlı, O. Şimşek, A. Yetgin, E.M. Altuner. "Determination of the chemical composition and antimicrobial activity of Frankenia hirsuta." Bangladesh Journal of Pharmacology, vol. 12, no. 4, pp. 463-469, 2017.
12. [12] M.Alpay, G.Dulger and E. Karabacak. “Antioxidant, Antimicrobial and Antitumoral Effects of Stachys annua (L.) L. subsp. annua var. annua in Comparative Cancer Profiles.” Indian Journal of Medical Research and Pharmaceutical Sciences, vol. 4, no. 12, pp. 68-74, 2017.
13. [13] K. Alipieva, L. Evstatieva, N. Handjieva, S. Popova. "Comparative analysis of the composition of flower volatiles from Lamium L. species and Lamiastrum galeobdolon Heist. ex Fabr." Zeitschrift für Naturforschung C, vol. 58, no. 11-12, pp. 779-782, 2003.
14. [14] G. Flamini, P.L.Cioni, I. Morelli. (2005). “Composition of the essential oils and in vivo emission of volatiles of four Lamium species from Italy: L. purpureum, L. hybridum, L. bifidum and L. amplexicaule. ” Food Chemistry, vol. 91, no. 1, pp. 63-68, 2005.
15. [15] C.D.Jones, K.E. Woods, W.N. Setzer. “A chemical ecological investigation of the allelopathic potential of Lamium amplexicaule and Lamium purpureum.” Open Journal of Ecology, vol. 2, no.4, pp. 167-177, 2012.
16. [16] Anonymous. (2019, 04 April). [Online]. Available: https://pubchem.ncbi.nlm.nih.gov/compound/tetradecanal
17. [17] K. Morteza-Semnani, M. Saeedi, M. Akbarzadeh. “Chemical Composition of the Essential Oil of the Flowering Aerial Parts of Lamium album L.” Journal of Essential Oil Bearing Plants, vol. 19, no. 3, pp. 773-777, 2016.
18. [18] A. Iordache, M. Culea, C. Gherman and O. Cozar. “Characterization of some plant extracts by GC–MS.” Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms, vol. 267, no. 2, pp. 338-342, 2009.
19. [19] C.L. Prins, I.J. Vieira and S.P. Freitas. “Growth regulators and essential oil production.” Brazilian Journal of Plant Physiology, vol. 22, no. 2, pp. 91-102, 2010.
20. [20] M.T. Selvi, R. Thirugnanasampandan and S. Sundarammal. “Antioxidant and cytotoxic activities of essential oil of Ocimum canum Sims. from India.” Journal of Saudi Chemical Society, vol. 19, no. 1, pp. 97-100, 2015.
21. [21] H. Harada, U. Yamashita, H. Kurihara, E. Fukushi, J. Kawabata, Y. Kamei. "Antitumor activity of palmitic acid found as a selective cytotoxic substance in a marine red alga." Anticancer Research, vol. 22, no. 5, pp. 2587-2590, 2002.