In Vitro Antimicrobial Activity Screening of Leucoagaricus leucothites and Determination of the Ethanol Extract Composition By Gas Chromatography/Mass Spectrometry

Abstract

Macrofungi are good food resources, which have not only medicinal properties but are nutritive as well. For centuries they have been used for preventing several diseases including cancer, hypercholesterolemia and hypertension. They are also known to present antimicrobial activity, thus the aim of the present study is to put forward antimicrobial activity of ethanol extract of Leucoagaricus leucothites (Vittad.) Wasser 1977, a macro fungi and in addition to determine the chemical composition by Gas Chromatography-Mass Spectrometry. Nineteen bacteria strains and one yeast strain are used in antimicrobial screening. According to the data obtained from the study L. leucothites presented both antibacterial and antifungal activity against the bacteria and yeast strains used at different concentrations with different levels. Although there are some previous studies, it can be proposed that this study is the first detailed screening report regarding the antibacterial and antifungal potential of L. leucothites and the compounds found in L. leucothites ethanol extract.

Keywords

• Leucoagaricus leucothites,Mushroom,Antimicrobial activity,Chemical composition,Disk diffusion method,GC-MS,GC-MS

Leucoagaricus leucothites’in In Vitro Antimikrobiyal Aktivite Taraması ve Etanol Ekstrakt Kompozisyonunun Gaz Kromatografisi/Kütle Spektrometresi ile Belirlenmesi

Abstract

Makro mantarlar sadece tıbbi özelliklere sahip olmayan, aynı zamanda da besleyici olan iyi besin kaynaklarıdır. Yüzyıllar boyunca kanser, hiperkolesterolemi ve hipertansiyon gibi çeşitli hastalıkları önlemek için kullanılmışlardır. Ayrıca makro mantarların antimikrobiyal aktivite gösterdikleri de bilinmektedir, bu nedenle bu çalışmanın ana amacı, bir makro mantar olan Leucoagaricus leucothites (Vittad.) Wasser 1977’nin etanol ekstraktının antimikrobiyal aktivitesini ortaya koymak ve ayrıca Gaz Kromatografisi-Kütle Spektrometresi ile kimyasal bileşimin belirlenmesini sağlamaktır. Antimikrobiyal taramada on dokuz bakteri, bir adet maya suşu kullanılmıştır. Bu çalışmadan elde edilen verilere göre L. leucothites farklı konsantrasyonlarda kullanılan tüm mikroorganizmalara karşı farklı seviyelerde hem antibakteriyel hem de antifungal aktivite ortaya koymuştur. Her ne kadar daha önce yapılmış birkaç çalışma bulunsa da; bu çalışmanın, L. leucothites'in antibakteriyel ve antifungal potansiyeli ve L. leucothites etanol ekstraktında bulunan bileşikler ile ilgili ilk ayrıntılı tarama raporu olduğu öne sürülebilir.

Keywords

• Leucoagaricus leucothites,Mantar,Antimikrobiyal aktivite,Kimyasal bileşim,Disk difüzyon yöntemi,GC-MS,GC-MS

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