In Vitro Antioxidant and Enzyme Inhibition Activity of Hesperis İsatidea (Boiss.) D.A. German & Al-Shehbaz (Brassicaceae) and its Anatomy

Gülnur Ekşi; Gülderen Yılmaz; Nuraniye Eruygur; Fatma Ayaz

doi:10.29130/dubited.931454

EN TR

In Vitro Antioxidant and Enzyme Inhibition Activity of Hesperis İsatidea (Boiss.) D.A. German & Al-Shehbaz (Brassicaceae) and its Anatomy

Abstract

Hesperis isatidea (Brassicaceae) is a perennial herbaceous endemic plant known as “Allıgelin”. It has traditional use concerning the cough, wound, cold and vulnerary in the form of decoction and poultice in Eastern Turkey. In this study, the in vitro antioxidant activity (DPPH, ABTS, FRAP, and metal chelating) and enzyme inhibitory activities (anticholinesterase, anti-tyrosinase) of H. isatideae were evaluated. Besides, the transverse sections were taken from the leaf, stem and fruit to determine the morphological and anatomical features of H. isatidea. The characteristic elements of the preparations were determined by Leica CME stereomicroscope and the distinctive features were shown in photographs. The obtained results of in vitro antioxidant and enzyme inhibitory assays suggest that H. isatidea may useful to develop bioactive compounds rich nutraceuticals for health care.

Keywords

Phenolics, anatomy, DPPH, ABTS, FRAP, Metal chelating, Anticholinesterase and anti-tyrosinase

Hesperis Isatidea (Boiss.) D.A. German & Al-Shehbaz (Brassicaceae) Türünün in vitro Antioksidan ve Enzim Aktivitesi ve Anatomisi

Abstract

Endemik bir tür olan Hesperis isatidea (Brassicaceae) “Allıgelin” olarak bilinen çok yıllık ve otsu bir bitkidir. Doğu Anadolu’da öksürükte, yara iyileştirmede, soğuk algınlığında dekoksisyon ya da lapa olarak geleneksel kullanımlarına rastlanmaktadır. Bu çalışmada, H. isatidea türünün in vitro antioksidan (DPPH, ABTS, FRAP ve metal bağlama) ve enzim inhibe edici aktiviteleri (antikolinesteraz, anti-tirozinaz) değerlendirilmiştir. Ayrıca H. isatidea türünün morfolojik ve anatomik özelliklerini aydınlatmak üzere yaprak, gövde ve meyveden enine kesit alınarak türün karakteristik unsurları Leica CME stereomikroskop ile belirlenmiş ve ayırt edici özellikleri fotoğraflanmıştır. In vitro antioksidan ve enzim inhibe edici deneylerin sonuçlarına göre, H. isatidea türünün sağlığa faydalı biyoaktif bileşikler açısından zengin olduğu ve nutrasötik geliştirme çalışmalarına katkı sağlayacağı düşünülmektedir.

Keywords

Fenolik bileşikler, Anatomy, DPPH, ABTS, FRAP, Metal bağlama, Antikolinesteraz ve anti-tirozinaz

References

[1] S. Aras, A. Duran, G. Yenilmez, and D. C. Duman, "Genetic relationships among some Hesperis L. (Brassicaceae) species from Turkey assessed by RAPD analysis," African Journal of Biotechnology, vol. 8, pp. 3128–3134, 2009.
[2] A. Duran, "Two New Species With Pendulous Fruits in Hesperis (Brassicaceae) from South Anatolia, Turkey," A Journal for Botanical Nomenclature. vol. 18, pp. 453–463, 2008.
[3] A. E. Farouji, H. Khodayari, and M. Assadi, "Taxonomic significance of pollen and seed micromorphology in the genus Hesperis L. (Brassicaceae)," Iranian Journal of Botany. vol. 24, pp. 91–104, 2018.
[4] A. E. Farouji, H. Khodayari, M. Assadi, B. Özüdoğru, Ö. Çetin, K. Mummenhoff, and S. Bhattacharya," Numerical taxonomy contributes to delimitation of Iranian and Turkish Hesperis L. (brassicaceae) species," Phytotaxa, vol. 367, pp. 101–119, 2018.
[5] A. Duran and Ö. Çetin, "New species and a synonym of the genus Hesperis (Brassicaceae) from Turkey," Turkish Journal of Botany, vol. 40, pp. 87–96, 2016.
[6] J. Cullen, “Hesperis L.,” in Flora Turkey East Aegean Islands, P.H. Davis,ed., Edinburgh, UK: Edinburgh University Press, 1965, vol. 1, pp. 452–460.
[7] A. Duran and A. Ocak, "Hesperis turkmendaghensis (sect. Hesperis) (Cruciferae/Brassicaceae), a new species from the Central Anatolia region, Turkey," Botanical Journal of the Linnean Society, vol. 147, pp. 239–247, 2005.
[8] O. Appel and I. A. Al-Shehbaz, Cruciferae. Flowering Plants Dicotyledons, Heidelberg, Berlin, Springer, 2003, pp. 75–174.
[9] D. A. German and I. A. Al-Shehbaz, "A reconsideration of Pseudofortuynia and Tchihatchewia as synonyms of sisymbrium and hesperis, respectively (Brassicaceae)," Phytotaxa, vol. 334, pp. 95–98, 2018.
[10] S. I. Warwick, K. Mummenhoff, C. A. Sauder, M. A. Koch, and I. A. Al-Shehbaz, "Closing the gaps: Phylogenetic relationships in the Brassicaceae based on DNA sequence data of nuclear ribosomal ITS," Plant Systematics and Evolution, vol. 285, pp. 209–232, 2010.

[11] E. Tuzlacı and A. Doǧan, "Turkish folk medicinal plants, IX: Ovacık (Tunceli) IX," Marmara Pharmaceutical Journal, vol. 14, pp. 136–143, 2010.
[12] M. Korkmaz and E. Karakurt, "An ethnobotanical ınvestigation to determine plants used as folk medicine in Kelkit (Gümüşhane/Turkey) district," Biological Diversity and Conservation, vol. 8, pp. 290–303, 2015.
[13] N. Kirimer, S. Gökmen, B. Demirci, K. H. C. Baser, and A. Duran, "Volatile compounds of seeds of Hesperis bicuspidata, H. bottae and H. podocarpa,"Journal of Essential Oil Research, vol. 22, pp. 230–231, 2010.
[14] S. Ozlem Sener, M. Badem, S. Kanbolat, N. Korkmaz, U. Ozgen, R. Aliyazicioglu, S. A. Karaoglu, and S. Terzioglu, " The Therapeutic Potency of Hesperis matronalis subsp. matronalis for Alzheimer’s and Microbial Diseases," Süleyman Demirel Üniversitesi Sağlık Bilimleri Dergisi, vol. 12, pp. 38–43, 2021.
[15] G. Clarke, K. N. Ting, C. Wiart, and J. Fry, "High correlation of 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, ferric reducing activity potential and total phenolics content indicates redundancy in use of all three assays to screen for antioxidant activity of extracts of plants from the m," Antioxidants, vol. 2, pp. 1–10, 2013.
[16] H. Yang, Y. Dong, H. Du, H. Shi, Y. Peng, and X. Li, "Antioxidant compounds from propolis collected in Anhui, China," Molecules, vol. 16, pp. 3444–3455, 2011.
[17] R. Re, N. Pellegrini, A. Proteggente, A. Pannala, M. Yang, and C. Rice-Evans, "Antioxidant activity applying an improved ABTS radical cation decolorization assay," Free Radical Biology and Medicine, vol. 26, pp. 1231–1237, 1999.
[18] S. H. Jeong, Y. B. Ryu, M. J. Curtis-Long, H. W. Ryu, Y. S. Baek, J. E. Kang, W. S. Lee, and K. H. Park, "Tyrosinase inhibitory polyphenols from roots of Morus Ihou," Journal of Agricultural and Food Chemistry, vol. 57, pp. 1195–1203, 2009.
[19] Z. Yang, Y. Wang, Y. Weng, and Y. Zhang, "Bioassay-guided screening and isolation of α-glucosidase and tyrosinase inhibitors from leaves of Morus alba," Food Chemistry, vol. 131, pp. 617–625, 2012.
[20] G. L. Ellman, K. D. Courtney, V. Andres, and R. M. Featherstone, "A new and rapid colorimetric determination of acetylcholinesterase activity," Biochemical Pharmacology, vol. 7, pp. 88–95, 1961.
[21] G. Zengin, H. H. Abdallah, A. Dogan, A. Mollica, Z. Aumeeruddy-Elalfi, and M. F. Mahomoodally, "Phenolic components and assessment of biological properties of Tchihatchewia isatidea Boiss. extracts: Docking and functional approaches for designing novel products," Food and Chemical Toxicology, vol. 111, pp. 423–431, 2018.

Details

Primary Language

English

Subjects

Engineering

Journal Section

Research Article

Authors

Gülnur Ekşi ^*
0000-0002-6869-8703
Türkiye

Gülderen Yılmaz
0000-0002-6569-4766
Türkiye

Nuraniye Eruygur
0000-0002-4674-7009
Türkiye

Fatma Ayaz
0000-0003-3994-6576
Türkiye

Publication Date

July 31, 2021

Submission Date

May 2, 2021

Acceptance Date

May 17, 2021

Published in Issue

Year 2021 Volume: 9 Number: 4

DOI

https://doi.org/10.29130/dubited.931454

IZ

https://izlik.org/JA99BL28ST

APA

Ekşi, G., Yılmaz, G., Eruygur, N., & Ayaz, F. (2021). In Vitro Antioxidant and Enzyme Inhibition Activity of Hesperis İsatidea (Boiss.) D.A. German & Al-Shehbaz (Brassicaceae) and its Anatomy. Duzce University Journal of Science and Technology, 9(4), 1483-1492. https://doi.org/10.29130/dubited.931454

AMA

1.Ekşi G, Yılmaz G, Eruygur N, Ayaz F. In Vitro Antioxidant and Enzyme Inhibition Activity of Hesperis İsatidea (Boiss.) D.A. German & Al-Shehbaz (Brassicaceae) and its Anatomy. DUBİTED. 2021;9(4):1483-1492. doi:10.29130/dubited.931454

Chicago

Ekşi, Gülnur, Gülderen Yılmaz, Nuraniye Eruygur, and Fatma Ayaz. 2021. “In Vitro Antioxidant and Enzyme Inhibition Activity of Hesperis İsatidea (Boiss.) D.A. German & Al-Shehbaz (Brassicaceae) and Its Anatomy”. Duzce University Journal of Science and Technology 9 (4): 1483-92. https://doi.org/10.29130/dubited.931454.

EndNote

Ekşi G, Yılmaz G, Eruygur N, Ayaz F (July 1, 2021) In Vitro Antioxidant and Enzyme Inhibition Activity of Hesperis İsatidea (Boiss.) D.A. German & Al-Shehbaz (Brassicaceae) and its Anatomy. Duzce University Journal of Science and Technology 9 4 1483–1492.

IEEE

[1]G. Ekşi, G. Yılmaz, N. Eruygur, and F. Ayaz, “In Vitro Antioxidant and Enzyme Inhibition Activity of Hesperis İsatidea (Boiss.) D.A. German & Al-Shehbaz (Brassicaceae) and its Anatomy”, DUBİTED, vol. 9, no. 4, pp. 1483–1492, July 2021, doi: 10.29130/dubited.931454.

ISNAD

Ekşi, Gülnur - Yılmaz, Gülderen - Eruygur, Nuraniye - Ayaz, Fatma. “In Vitro Antioxidant and Enzyme Inhibition Activity of Hesperis İsatidea (Boiss.) D.A. German & Al-Shehbaz (Brassicaceae) and Its Anatomy”. Duzce University Journal of Science and Technology 9/4 (July 1, 2021): 1483-1492. https://doi.org/10.29130/dubited.931454.

JAMA

1.Ekşi G, Yılmaz G, Eruygur N, Ayaz F. In Vitro Antioxidant and Enzyme Inhibition Activity of Hesperis İsatidea (Boiss.) D.A. German & Al-Shehbaz (Brassicaceae) and its Anatomy. DUBİTED. 2021;9:1483–1492.

MLA

Ekşi, Gülnur, et al. “In Vitro Antioxidant and Enzyme Inhibition Activity of Hesperis İsatidea (Boiss.) D.A. German & Al-Shehbaz (Brassicaceae) and Its Anatomy”. Duzce University Journal of Science and Technology, vol. 9, no. 4, July 2021, pp. 1483-92, doi:10.29130/dubited.931454.

Vancouver

1.Gülnur Ekşi, Gülderen Yılmaz, Nuraniye Eruygur, Fatma Ayaz. In Vitro Antioxidant and Enzyme Inhibition Activity of Hesperis İsatidea (Boiss.) D.A. German & Al-Shehbaz (Brassicaceae) and its Anatomy. DUBİTED. 2021 Jul. 1;9(4):1483-92. doi:10.29130/dubited.931454

Cited By

Total Phenolic, Flavonoid Contents, Antioxidant and Antimicrobial Activities of Hesperis pendula

Prospects in Pharmaceutical Sciences

https://doi.org/10.56782/pps.135

Chemical Composition and Antimicrobial Activity of Hesperis isatidea (Boiss.) D.A.German & Al‐Shehbaz Essential Oil

Chemistry & Biodiversity

https://doi.org/10.1002/cbdv.202500455

Molecular Characterization and Genotypes Structure Analysis of Anatolian Endemic Hesperis isatidea (Brassicaceae) Using of Inter-Simple Sequence Repeat (ISSR) Markers

Biology Bulletin

https://doi.org/10.1134/S1062359025610055