In Vitro Antioxidant and Enzyme Inhibition Activity of Hesperis İsatidea (Boiss.) D.A. German & Al-Shehbaz (Brassicaceae) and its Anatomy

Abstract

Hesperis isatidea (Brassicaceae) is a perennial herbaceous endemic plant known as “Allıgelin”. It has traditional use concerning the cough, wound, cold and vulnerary in the form of decoction and poultice in Eastern Turkey. In this study, the in vitro antioxidant activity (DPPH, ABTS, FRAP, and metal chelating) and enzyme inhibitory activities (anticholinesterase, anti-tyrosinase) of H. isatideae were evaluated. Besides, the transverse sections were taken from the leaf, stem and fruit to determine the morphological and anatomical features of H. isatidea. The characteristic elements of the preparations were determined by Leica CME stereomicroscope and the distinctive features were shown in photographs. The obtained results of in vitro antioxidant and enzyme inhibitory assays suggest that H. isatidea may useful to develop bioactive compounds rich nutraceuticals for health care.

Keywords

• Phenolics
• anatomy
• DPPH
• ABTS
• FRAP
• Metal chelating
• Anticholinesterase and anti-tyrosinase

Hesperis Isatidea (Boiss.) D.A. German & Al-Shehbaz (Brassicaceae) Türünün in vitro Antioksidan ve Enzim Aktivitesi ve Anatomisi

Abstract

Endemik bir tür olan Hesperis isatidea (Brassicaceae) “Allıgelin” olarak bilinen çok yıllık ve otsu bir bitkidir. Doğu Anadolu’da öksürükte, yara iyileştirmede, soğuk algınlığında dekoksisyon ya da lapa olarak geleneksel kullanımlarına rastlanmaktadır. Bu çalışmada, H. isatidea türünün in vitro antioksidan (DPPH, ABTS, FRAP ve metal bağlama) ve enzim inhibe edici aktiviteleri (antikolinesteraz, anti-tirozinaz) değerlendirilmiştir. Ayrıca H. isatidea türünün morfolojik ve anatomik özelliklerini aydınlatmak üzere yaprak, gövde ve meyveden enine kesit alınarak türün karakteristik unsurları Leica CME stereomikroskop ile belirlenmiş ve ayırt edici özellikleri fotoğraflanmıştır. In vitro antioksidan ve enzim inhibe edici deneylerin sonuçlarına göre, H. isatidea türünün sağlığa faydalı biyoaktif bileşikler açısından zengin olduğu ve nutrasötik geliştirme çalışmalarına katkı sağlayacağı düşünülmektedir.

Keywords

• Fenolik bileşikler
• Anatomy
• DPPH
• ABTS
• FRAP
• Metal bağlama
• Antikolinesteraz ve anti-tirozinaz

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