Effect the some heavy metals on carbonic anhydrase enzymes activities from non-tumour and tumour human stomach

Emrah Yerlikaya; Ramazan Demirdağ; Ömer Küfrevioğlu; Cemal Gündoğdu

Effect the some heavy metals on carbonic anhydrase enzymes activities from non-tumour and tumour human stomach

Abstract

In this study, in vitro effects of certain heavy metals on the human carbonic anhydrase enzyme were examined. Inhibitory effects of metal ions ( Pb²⁺, Cu²⁺, Fe²⁺,Cr²⁺, Al³⁺, Ni²⁺, Mn²⁺, Cd²⁺, Zn²⁺, and Mg²⁺) were observed in tumour and non-tumour tissue. IC₅₀ values were calculated for metals. The Cu²⁺, Zn²⁺, Ni²⁺, Cd²⁺ and Mg²⁺IC₅₀ values of tumour tissue were calculated as 0.034mM, 0.426mM, 0.597mM, 0.878mM and 2.52mM respectively. The Cu²⁺, Zn²⁺, Ni²⁺, Cd²⁺ and Mg²⁺IC₅₀ values of non-tumour tissue were calculated as 0.067mM, 0.991mM, 1.065mM, 1.724mM and 6.13mM respectively. Carbonic anhydrase activity was measured as described by Wilbur and Anderson. Hydratase activity was used to determine IC₅₀ values. In this study, tumour and non-tumour human stomach tissue was selected due to the fact that among the diseases, stomach cancer has one of the highest mortality rates. Stomach cancer, a type of cancer effecting the digestive system, is a fatal disease in living systems. The effects of metals on the CA enzyme were investigated due to the extremely important role that CA enzymes play in living beings.

Keywords

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References

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Details

Primary Language

Turkish

Subjects

-

Journal Section

-

Authors

Emrah Yerlikaya This is me

Ramazan Demirdağ

Ömer Küfrevioğlu

Cemal Gündoğdu This is me

Publication Date

May 20, 2015

Submission Date

April 2, 2015

Acceptance Date

-

Published in Issue

Year 2015 Volume: 1 Number: 1

IZ

https://izlik.org/JA68ZH52UY

Cite

RIS / Bibtex

APA

Yerlikaya, E., Demirdağ, R., Küfrevioğlu, Ö., & Gündoğdu, C. (2015). Effect the some heavy metals on carbonic anhydrase enzymes activities from non-tumour and tumour human stomach. Eastern Anatolian Journal of Science, 1(1), 25-28. https://izlik.org/JA68ZH52UY

AMA

1.Yerlikaya E, Demirdağ R, Küfrevioğlu Ö, Gündoğdu C. Effect the some heavy metals on carbonic anhydrase enzymes activities from non-tumour and tumour human stomach. Eastern Anatolian Journal of Science. 2015;1(1):25-28. https://izlik.org/JA68ZH52UY

Chicago

Yerlikaya, Emrah, Ramazan Demirdağ, Ömer Küfrevioğlu, and Cemal Gündoğdu. 2015. “Effect the Some Heavy Metals on Carbonic Anhydrase Enzymes Activities from Non-Tumour and Tumour Human Stomach”. Eastern Anatolian Journal of Science 1 (1): 25-28. https://izlik.org/JA68ZH52UY.

EndNote

Yerlikaya E, Demirdağ R, Küfrevioğlu Ö, Gündoğdu C (May 1, 2015) Effect the some heavy metals on carbonic anhydrase enzymes activities from non-tumour and tumour human stomach. Eastern Anatolian Journal of Science 1 1 25–28.

IEEE

[1]E. Yerlikaya, R. Demirdağ, Ö. Küfrevioğlu, and C. Gündoğdu, “Effect the some heavy metals on carbonic anhydrase enzymes activities from non-tumour and tumour human stomach”, Eastern Anatolian Journal of Science, vol. 1, no. 1, pp. 25–28, May 2015, [Online]. Available: https://izlik.org/JA68ZH52UY

ISNAD

Yerlikaya, Emrah - Demirdağ, Ramazan - Küfrevioğlu, Ömer - Gündoğdu, Cemal. “Effect the Some Heavy Metals on Carbonic Anhydrase Enzymes Activities from Non-Tumour and Tumour Human Stomach”. Eastern Anatolian Journal of Science 1/1 (May 1, 2015): 25-28. https://izlik.org/JA68ZH52UY.

JAMA

1.Yerlikaya E, Demirdağ R, Küfrevioğlu Ö, Gündoğdu C. Effect the some heavy metals on carbonic anhydrase enzymes activities from non-tumour and tumour human stomach. Eastern Anatolian Journal of Science. 2015;1:25–28.

MLA

Yerlikaya, Emrah, et al. “Effect the Some Heavy Metals on Carbonic Anhydrase Enzymes Activities from Non-Tumour and Tumour Human Stomach”. Eastern Anatolian Journal of Science, vol. 1, no. 1, May 2015, pp. 25-28, https://izlik.org/JA68ZH52UY.

Vancouver

1.Emrah Yerlikaya, Ramazan Demirdağ, Ömer Küfrevioğlu, Cemal Gündoğdu. Effect the some heavy metals on carbonic anhydrase enzymes activities from non-tumour and tumour human stomach. Eastern Anatolian Journal of Science [Internet]. 2015 May 1;1(1):25-8. Available from: https://izlik.org/JA68ZH52UY