Araştırma Makalesi
BibTex RIS Kaynak Göster
Yıl 2023, , 26 - 30, 27.06.2023
https://doi.org/10.46239/ejbcs.1276554

Öz

Kaynakça

  • Anderson IC, Parkin PI. 2007. Detection of active soil fungi by RT-PCR amplification of precursor rRNA molecules. Microbiol Methods. 68:248–253.
  • Bermingham S, Maltby L, Cooke RC. 1996. Effect of acoal mine effluent on aquatic hyphomycetes. Field Study Appl Ecol. 33: 1311-1321.
  • Cappccino JG, Sherman N. 1996. Microbiology. alaboratory manual. 4th edu. Benjamin. Cummings pub.
  • Chandrashekar MA, Soumya Pai K, Raju NS. 2014. Fungal Diversity of Rhizosphere Soils in Different Agricultural fields of Nanjangud Taluk of Mysore District. Karnataka. India. Int.J.Curr.Microbiol.App.Sci. 3:559-566.
  • Gaddeyya G, Niharika PS, Bharathi P, Kumar PKR. 2012. Isolation and identification of soil mycoflora in different crop fields at Salur Mandal. AdvAppl Sci Res. 3:2020-2026.
  • Gravesen S, Frisvad JC, Samson RA. 1994. Microfungi. Munksgaard Publishers. Copenhagen.
  • Jasuja ND, Saxena R, Chandra S, Joshi SC. 2013. Isolation and identification of microorganism from polyhouse agriculture soil of Rajasthan. African J Microbiol Res. 7: 4886-4891. Javadi MA, Ghanbary MAT, Tazick Z. 2012. Isolation and molecular identification of soil inhabitant Penicillia. Ann of Biol Res. 3: 5758-5761. Kamel T. 2013. Mesopotamia in Mesopotamia. Iraqi Nation Studies Center. www.mesopotamia4374.com/dose/ Mesopotamia in Mesopotamia. Iraqi Nation Studies Center. Accessed 20 Oct 2013.
  • Lutzoni F, Kauff F, Cox CJ, Laughlin D, Celio G. 2004. Assembling the fungal tree of life progress classification and evolution of the subcellular traits. Am J Bot. 91: 1446-1480.
  • Nollet LM. 2007. Handbook of water analysis. 2PndP ed. CRC Press. London.
  • Pellon A, Sadeghi N, Moyes DL. 2020. New insights in Candida albicans innate immunity at the mucosa toxins epithelium metabolism and beyond. Frontiers in cellular and infection microbiology.10-81.
  • Reddy PL, Babu BS, Radhaiah A, Sreeramulu A. 2014. Screening, identification and isolation of cellulolytic fungi from soils of Chittoor District. India. Int J Curr Microbiol Appl Sci. 3: 761-771. Sharma MS, Raju NS. 2013. Frequency and percentage occurrence of soil mycoflora in different crop fields at H D Kote of Mysore district. Inter J Environ Sci. 3: 1569-1576.
  • Wang JH, Zhang HP, gong S, Xue RS, Agboola Y, Liao C. 2014. Molecular identification mycotoxin production and comparative pathogenicity of Fusariumtemperatum isolated from maize in China. J. Phytopathol. 162:147-157.
  • Wang Z, Nilsson RH, James TY, Dai Y, Townsend JP. 2016. Biology of Microfungi. pp 25−46.
  • Webster J, Weber RA. 2007. Introduction to fungi. In: Jack S. Fungi, 3rd edn. New York.

Morphological and molecular identification of fungi isolated from various habitat in Kirkuk city – Iraq

Yıl 2023, , 26 - 30, 27.06.2023
https://doi.org/10.46239/ejbcs.1276554

Öz

The aim of this study is isolate and identify fungi from different habitat in Kirkuk City - Iraq. The fungal species were isolated from soil and water in four season 2021-2022, collected the samples from various geographical habitat in Kirkuk City. The fungi isolation from soil and water done by inoculating (1ml) from serial dilutions on Potato Dextrose Agar (PDA) plates. The molecular identification of the isolated fungi at the species level, by PCR using specific internal transcribed spacer primer (ITS1/ITS4). The PCR products were sequenced and compared with the other related sequences in GenBank (NCBI). Seven fungal species were identified. The results showed that the (Aspergillus flavus 20.83%) was the most abundant fungus, while the (Penicillium citrinum 8.30%) was the less prevalent one in all resources and locations. The seven local fungal isolates were registered within NCBI, and this is the first record of these isolates in Iraq

Kaynakça

  • Anderson IC, Parkin PI. 2007. Detection of active soil fungi by RT-PCR amplification of precursor rRNA molecules. Microbiol Methods. 68:248–253.
  • Bermingham S, Maltby L, Cooke RC. 1996. Effect of acoal mine effluent on aquatic hyphomycetes. Field Study Appl Ecol. 33: 1311-1321.
  • Cappccino JG, Sherman N. 1996. Microbiology. alaboratory manual. 4th edu. Benjamin. Cummings pub.
  • Chandrashekar MA, Soumya Pai K, Raju NS. 2014. Fungal Diversity of Rhizosphere Soils in Different Agricultural fields of Nanjangud Taluk of Mysore District. Karnataka. India. Int.J.Curr.Microbiol.App.Sci. 3:559-566.
  • Gaddeyya G, Niharika PS, Bharathi P, Kumar PKR. 2012. Isolation and identification of soil mycoflora in different crop fields at Salur Mandal. AdvAppl Sci Res. 3:2020-2026.
  • Gravesen S, Frisvad JC, Samson RA. 1994. Microfungi. Munksgaard Publishers. Copenhagen.
  • Jasuja ND, Saxena R, Chandra S, Joshi SC. 2013. Isolation and identification of microorganism from polyhouse agriculture soil of Rajasthan. African J Microbiol Res. 7: 4886-4891. Javadi MA, Ghanbary MAT, Tazick Z. 2012. Isolation and molecular identification of soil inhabitant Penicillia. Ann of Biol Res. 3: 5758-5761. Kamel T. 2013. Mesopotamia in Mesopotamia. Iraqi Nation Studies Center. www.mesopotamia4374.com/dose/ Mesopotamia in Mesopotamia. Iraqi Nation Studies Center. Accessed 20 Oct 2013.
  • Lutzoni F, Kauff F, Cox CJ, Laughlin D, Celio G. 2004. Assembling the fungal tree of life progress classification and evolution of the subcellular traits. Am J Bot. 91: 1446-1480.
  • Nollet LM. 2007. Handbook of water analysis. 2PndP ed. CRC Press. London.
  • Pellon A, Sadeghi N, Moyes DL. 2020. New insights in Candida albicans innate immunity at the mucosa toxins epithelium metabolism and beyond. Frontiers in cellular and infection microbiology.10-81.
  • Reddy PL, Babu BS, Radhaiah A, Sreeramulu A. 2014. Screening, identification and isolation of cellulolytic fungi from soils of Chittoor District. India. Int J Curr Microbiol Appl Sci. 3: 761-771. Sharma MS, Raju NS. 2013. Frequency and percentage occurrence of soil mycoflora in different crop fields at H D Kote of Mysore district. Inter J Environ Sci. 3: 1569-1576.
  • Wang JH, Zhang HP, gong S, Xue RS, Agboola Y, Liao C. 2014. Molecular identification mycotoxin production and comparative pathogenicity of Fusariumtemperatum isolated from maize in China. J. Phytopathol. 162:147-157.
  • Wang Z, Nilsson RH, James TY, Dai Y, Townsend JP. 2016. Biology of Microfungi. pp 25−46.
  • Webster J, Weber RA. 2007. Introduction to fungi. In: Jack S. Fungi, 3rd edn. New York.
Toplam 14 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Yapısal Biyoloji
Bölüm Araştırma Makaleleri
Yazarlar

Yavuz Mahmut

Abdul-hameed M. Hamoody Bu kişi benim 0000-0002-4333-8461

Rushdi Sabah Abdulqader 0000-0002-7636-6303

Yayımlanma Tarihi 27 Haziran 2023
Kabul Tarihi 11 Mayıs 2023
Yayımlandığı Sayı Yıl 2023

Kaynak Göster

APA Mahmut, Y., M. Hamoody, A.-h., & Abdulqader, R. S. (2023). Morphological and molecular identification of fungi isolated from various habitat in Kirkuk city – Iraq. Eurasian Journal of Biological and Chemical Sciences, 6(1), 26-30. https://doi.org/10.46239/ejbcs.1276554
AMA Mahmut Y, M. Hamoody Ah, Abdulqader RS. Morphological and molecular identification of fungi isolated from various habitat in Kirkuk city – Iraq. Eurasian J. Bio. Chem. Sci. Haziran 2023;6(1):26-30. doi:10.46239/ejbcs.1276554
Chicago Mahmut, Yavuz, Abdul-hameed M. Hamoody, ve Rushdi Sabah Abdulqader. “Morphological and Molecular Identification of Fungi Isolated from Various Habitat in Kirkuk City – Iraq”. Eurasian Journal of Biological and Chemical Sciences 6, sy. 1 (Haziran 2023): 26-30. https://doi.org/10.46239/ejbcs.1276554.
EndNote Mahmut Y, M. Hamoody A-h, Abdulqader RS (01 Haziran 2023) Morphological and molecular identification of fungi isolated from various habitat in Kirkuk city – Iraq. Eurasian Journal of Biological and Chemical Sciences 6 1 26–30.
IEEE Y. Mahmut, A.-h. M. Hamoody, ve R. S. Abdulqader, “Morphological and molecular identification of fungi isolated from various habitat in Kirkuk city – Iraq”, Eurasian J. Bio. Chem. Sci., c. 6, sy. 1, ss. 26–30, 2023, doi: 10.46239/ejbcs.1276554.
ISNAD Mahmut, Yavuz vd. “Morphological and Molecular Identification of Fungi Isolated from Various Habitat in Kirkuk City – Iraq”. Eurasian Journal of Biological and Chemical Sciences 6/1 (Haziran 2023), 26-30. https://doi.org/10.46239/ejbcs.1276554.
JAMA Mahmut Y, M. Hamoody A-h, Abdulqader RS. Morphological and molecular identification of fungi isolated from various habitat in Kirkuk city – Iraq. Eurasian J. Bio. Chem. Sci. 2023;6:26–30.
MLA Mahmut, Yavuz vd. “Morphological and Molecular Identification of Fungi Isolated from Various Habitat in Kirkuk City – Iraq”. Eurasian Journal of Biological and Chemical Sciences, c. 6, sy. 1, 2023, ss. 26-30, doi:10.46239/ejbcs.1276554.
Vancouver Mahmut Y, M. Hamoody A-h, Abdulqader RS. Morphological and molecular identification of fungi isolated from various habitat in Kirkuk city – Iraq. Eurasian J. Bio. Chem. Sci. 2023;6(1):26-30.