Studies on Anticholinesterase and Antioxidant Effects of Samples from Iris L. Genus of Turkish Origin

Abstract

The genus Iris L. (Iridaceae) is a member of geophytes with attractive flowers. There are about 56 Iris taxa growing in Turkey, 24 of which are endemic. A survey of the literature indicates that the research carried out on Iris species are focused on the flavonoid and volatile compounds of the plant.

In present study, the dichloromethane and methanol extracts prepared from the rhizomes of 47 Iris taxa growing in Turkey were investigated for their in vitro cholinesterase inhibitory effects against acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) which the enzymes linked to Alzheimer’s diseases and antioxidant capacities using 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging test as well.

The Iris extracts studied have been found more active against BChE than AChE. compared with 100 μg/ml galanthamine (89.29 ± 0.96 %) as reference, Iris kerneriana (coded as Y122) and Iris pseudacorus (coded as Y131) methanol extracts had significant BChE inhibition effect (respectively, 80.22 ± 1.04 % and 53.06 ± 1.13 %) at concentration of 200 μg/ml. Among tested samples, methanol extracts of I. kerneriana, I. lazica, I. pseudacorus and I. suaveolens have shown remarkable antioxidant activity at concentration of 2 mg/ml for DPPH compared with gallic acid.

Keywords

• Iris,Anticholinesterase,Antioxidant,Activity

Supporting Institution

TÜBİTAK

Project Number

KAMAG-110G007

Thanks

Turkish Scientific and Technological Research Council (TÜBİTAK) for financial source (Project code: KAMAG-110G007) was kindly acknowledged.

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