Farklı ışık yayan diyotlar (LED) altında tıbbi sucul bitki Lysimachia nummularia L.'nın boğum eksplantlarından çoklu sürgün rejenerasyonu

Yıl 2019, Cilt: 2 Sayı: 1, 11 - 16, 20.07.2019

Muhammet Dogan

Öz

Işık yayan diyotlar (LED) bitki büyüme ve gelişimi için
önemli avantajlara sahip olduklarından dolayı doku kültürü çalışmalarında
kullanılmaya başlanmıştır. LED’lerin ışık renkleri bitkiler üzerinde farklı
etkiler göstermektedir. Bu çalışmada, beyaz (B), kirmizi (K), mavi (M) LED
ışıkların L. nummularia'nın çoklu
sürgün rejenerasyonu üzerine tek ve kombine etkileri incelenmiştir. Boğum
eksplantları 0,10 mg/L indol butirik asit (IBA) ve 0,20-0,80 mg/L Thidiazuron
(TDZ) eklenmiş Murashige ve Skoog (MS) besin ortamında sekiz hafta süre ile
kültürlenmiştir. Her üç hormon uygulamasında %100 sürgün rejenerasyon
frekansları 2B:1K:1M ve 1B:1K:1M LED ışıkları altında kaydedilmiştir. En fazla
sürgün sayısı 1B:2K:1M LED ışıklar altında sırasıyla 17,47, 16,16 ve 13,46 adet
olarak sırasıyla 0,40 mg/L TDZ + 0,10 mg/L IBA, 0,20 mg/L TDZ + 0,10 mg/L IBA
ve 0,80 mg/L TDZ + 0,10 mg/L IBA eklenmiş MS besin ortamında belirlenmiştir. En
az sayıda sürgünler ise mavi LED altında kaydedilmiştir. En uzun sürgünler tüm
ortamlarda 1B:1K:2M LED ışık altındaki 
eksplantlardan elde edilmiştir. TDZ’nin kültür ortamında fazla
kullanılması sürgün uzunluğunu olumsuz etkilemiştir. Çoğaltım denemelerinde
yoğun kök oluştuğundan dolayı ayrıca köklendirme yapılmamıştır. Kültür
ortamında üretilen bitkiler dış koşullara başarıyla alıştırılmıştır. Sonuç
olarak çalışma, LED ışıkların L.
nummularia’nın üretimindeki etkinliğini ve flouresan ışıklara göre
üstünlüğünü göstermiştir.

Anahtar Kelimeler

Doku kültürü, Işık etkisi, LED, Shoot rejenerasyonu

Destekleyen Kurum

Türkiye Bilimsel ve Teknolojik Araştırma Kurumu (TÜBİTAK)

Proje Numarası

2130190

Teşekkür

Bu çalışma, Türkiye Bilimsel ve Teknolojik Araştırma Kurumu (TÜBİTAK) tarafından desteklenmiştir (Proje no:2130190).

Multiple shoot regeneration from nodal explants of medicinal aquatic plant Lysimachia nummularia L. under different light emitting diodes (LEDs)

Öz

Since light emitting diodes (LEDs) have important
advantages for plant growth and development, they have been used in tissue
culture studies. The light colours of LEDs show different effects on plants. In
this study, single and combined effects of white (W), red (R), blue (B) LED
lights on multiple shoot regeneration of Lysimachia
nummularia L. were investigated. Nodal explants were cultured for eight
weeks in a Murashige and Skoog (MS) nutrient medium supplemented with 0.10 mg/L
indole butyric acid (IBA) and 0.20-0.80 mg/L Thidiazuron (TDZ). 100% shoot
regeneration frequencies in all three hormone applications were recorded under
2W:1R: 1B and 1W: 1R: 1B LED lights. The maximum number of shoots was
determined under 1W:2R:1B LED lights as 17.47, 16.16 and 13.46 shoots/explant
in the MS nutrient medium added 0.40 mg/L TDZ + 0.10 mg/L IBA, 0.20 mg/L TDZ +
0.10 mg/L and 0.80 mg/L TDZ + 0.10 mg/L IBA, respectively. The minimum number
of shoots was recorded under blue LED. The longest shoot was obtained from
explants under 1W:1R:2B LED light in all environments. Excessive use of TDZ in
the culture medium adversely affected shoot length. Since there was a dense
root in replication experiments, no rooting was performed. Plants produced in
the culture environment have been successfully adapted to external conditions.
As a result, the study showed the effectiveness of the LED lights on the
production of L. nummularia and their
superiority over the fluorescent lights.

Anahtar Kelimeler

Tissue culture, Light effect, LED, Shoot regeneration

Proje Numarası

2130190

Kaynakça

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