Cytotoxic, genotoxic and anti-metastatic activity of Alkanna orientalis (L.) extract on glioblastoma multiforme brain cancer

Özge Çağlar Yıldırım; Sena Öner; Cihat Aksakal; Ceren Karagöz; Cemal Metehan Akkuş; Hasan Türkez

doi:10.5281/zenodo.17786112

EN

Cytotoxic, genotoxic and anti-metastatic activity of Alkanna orientalis (L.) extract on glioblastoma multiforme brain cancer

Abstract

This study aimed to evaluate, for the first time, the anti-proliferative and anti-metastatic effects of the aqueous extract of Alkanna orientalis (L.) on U87-MG glioblastoma cells. The cytotoxicity of this extract on normal human dermal fibroblasts and its anti-cancer activity on glioblastoma cells were assessed by using MTT assay. The potential genotoxic effects of the Alkanna orientalis (L.) extract on cell nuclei were further examined by Hoechst 33258 fluorescence staining and analyzed microscopically. In addition, the inhibitory effect of the extract at a selected dose on the metastatic capacity of glioblastoma cells was investigated using in vitro scratch assay. The plant extract demonstrated no significant cytotoxicity in HDF cells at any of the tested concentrations, whereas even the lowest tested dose exhibited notable anti-proliferative activity against U87-MG glioblastoma cells. Hoechst 33258 staining revealed no evidence of genotoxic nuclear alterations in HDF cells treated with 400 µg/mL extract compared to untreated controls. Moreover, the in vitro scratch assay revealed a pronounced inhibition of U87-MG cell migration 24 hours after treatment with this plant extract, indicating a potential anti-metastatic effect. These findings suggest that the aqueous extract of Alkanna orientalis (L.) may represent a promising alternative therapeutic candidate for the treatment of glioblastoma.

Keywords

glioblastoma , Alkanna orientalis , aqueous plant extract , anti-proliferative , anti-metastatic

References

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Details

Primary Language

English

Subjects

Plant Cell and Molecular Biology

Journal Section

Research Article

Authors

Özge Çağlar Yıldırım ^*
0000-0003-1412-8411
Türkiye

Sena Öner
0000-0001-7886-8561
Türkiye

Cihat Aksakal
0009-0003-7519-5500
Türkiye

Ceren Karagöz
0009-0004-6156-3488
Türkiye

Cemal Metehan Akkuş
0009-0007-8777-2853
Türkiye

Hasan Türkez
0000-0002-7046-8990
Türkiye

Early Pub Date

December 11, 2025

Publication Date

December 13, 2025

Submission Date

July 25, 2025

Acceptance Date

August 19, 2025

Published in Issue

Year 2025 Volume: 4 Number: 2

DOI

https://doi.org/10.5281/zenodo.17786112

IZ

https://izlik.org/JA33TP46TC

APA

Yıldırım, Ö. Ç., Öner, S., Aksakal, C., Karagöz, C., Akkuş, C. M., & Türkez, H. (2025). Cytotoxic, genotoxic and anti-metastatic activity of Alkanna orientalis (L.) extract on glioblastoma multiforme brain cancer. Eurasian Journal of Molecular and Biochemical Sciences, 4(2), 56-63. https://doi.org/10.5281/zenodo.17786112

Öz

Cytotoxic, genotoxic and anti-metastatic activity of Alkanna orientalis (L.) extract on glioblastoma multiforme brain cancer

Abstract

Keywords

References

Details

Primary Language

Subjects

Journal Section

Authors

Early Pub Date

Publication Date

Submission Date

Acceptance Date

Published in Issue

DOI

IZ

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