Comparison of Dental Follicle Stem Cells and Dental Pulp Stem Cells in a Translational Bone Tissue Engineering Protocol

Abstract

Purpose: In this study, it is aimed to establish and refine a translational protocol and compare the osteogenic potential of DPSC and DFSC’s on nano mesh containing (nmPCL) and plain (PCL) polycaprolactone scaffolds in vitro and contribute the translational medicine protocols in bone regeneration. Materials and Methods: DPSCs and DFSCs were osteogenically differentiated on PCL and nmPCL scaffolds and four groups were examined for cell proliferation and type I collagen formation rates after two weeks of culture. Following immunofluorescence labeling, Nonparametric (Kruskal Wallis) and multiple comparison tests were used to compare the four groups. Results: Among all groups, mean cell counts on scaffolds ranged from 30.8 to 82.6 cells/0.0915 mm2, and total collagen formation ranged from 2.79% to 17,9%. DFSC and nmPCL complex showed significantly higher cell counts (p< 0,01) and collagen formation rates (p< 0,01) in comparison to other groups. Conclusion: DFSC/nmPCL group is found to show superior properties on cell proliferation and bone matrix formation. This complex is a promising tool for maxillofacial tissue engineering applications.

Keywords

• Dental Follicle Stem Cell
• Dental Pulp Stem Cell
• Polycaprolactone
• Tissue Engineering
• Bone
• Translational Medicine

Translasyonel Kemik Doku Mühendisliği Protokolünde Dental Folikül Kök Hücreleri ile Dental Pulpa Kök Hücrelerinin Karşılaştırılması

Abstract

Amaç: Bu çalışmada, DPSC ve DFSC'lerin nanomesh içeren (nmPCL) ve düz (PCL) polikaprolakton iskeleler üzerindeki osteojenik potansiyellerini in vitro olarak karşılaştırmak ve kemik rejenerasyonunda translasyonel tıp protokollerine katkıda bulunmak amaçlanmıştır. Gereç ve Yöntemler: DPSC'ler ve DFSC'ler PCL ve nmPCL iskeleleri üzerinde osteojenik olarak farklılaştırılmış ve iki haftalık kültür sonrasında dört grup hücre proliferasyonu ve tip I kollajen oluşum oranları açısından incelenmiştir. İmmünofloresan etiketlemenin ardından, dört grubu karşılaştırmak için parametrik olmayan (Kruskal Wallis) ve çoklu karşılaştırma testleri kullanılmıştır. Sonuçlar: Tüm gruplar arasında, iskelelerdeki ortalama hücre sayıları 30.8 ila 82.6 hücre/0.0915 mm2 arasında ve toplam kolajen oluşumu %2.79 ila %17,9 arasında değişmektedir. DFSC ve nmPCL kompleksi diğer gruplara kıyasla anlamlı olarak daha yüksek hücre sayısı (p< 0,01) ve kolajen oluşum oranları (p< 0,01) göstermiştir. Sonuç: DFSC/nmPCL grubunun hücre proliferasyonu ve kemik matriksi oluşumu üzerinde üstün özellikler gösterdiği bulunmuştur. Bu kompleks maksillofasiyal doku mühendisliği uygulamaları için umut verici bir araçtır.

Keywords

• Diş Folikülü Kök Hücresi
• Diş Pulpası Kök Hücresi
• Polikaprolakton
• Doku Mühendisliği
• Kemik
• Translasyonel Tıp

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