Yıl 2021, Cilt 3 , Sayı 1, Sayfalar 16 - 20 2021-03-30

Determination of TNF-beta Marker in Intrauterine E. coli Induced Endometritis Model in Rats

Mustafa MAKAV [1] , Mushap KURU [2]


Objectives: This study aims to investigate the importance of tumor necrosis factor (TNF)-beta marker in the endometritis model created by E. coli.

Materials and Methods: Rats were divided into control and endometritis groups with 7 rats in each group in the study. All animals were fed as ad libitum. No application was made to rats in the control group. Progesterone (P4) was administered subcutaneously to rats in the experimental group at a daily dose of 16 mg/kg between the 0th and 4th days of the study. E. coli was administered intrauterinely at 25 μl and 1 × 105 CFU/rat on the 3rd day. Blood samples were taken at the end of the study after the lives of all animals were terminated in accordance with ethical rules. Hemogram analysis was performed for white blood cell (WBC) levels from the blood samples taken. The TNF-beta analysis was performed with a commercial kit by separating serum from blood samples.

Results: WBC (p=0.0004) and TNF-beta (p=0.027) were statistically higher in the endometritis group compared to the control group.

Conclusion: Consequently, increased TNF-beta in endometritis may be a diagnostic marker for inflammations in the genital tract. However, its effectiveness may be better demonstrated by conducting comprehensive studies with the relevant marker.

Amaç: Çalışmanın amacı, E. coli ile oluşturulan endometritis modelinde tümör nekroz faktör (TNF) -beta markırının öneminin araştırılması amaçlandı.

Gereç ve Yöntem: Çalışmada ratlar her grupta 7’şer rat olacak şekilde kontrol ve endometritis grubuna ayrıldı. Tüm hayvanlar ad libitum olarak beslendi. Kontrol gruplarına herhangi bir uygulama yapılmamıştır. Deneysel gruba ise Çalışmanın 0 ve 4. günleri arasında ratlara günlük subkutan 16 mg/kg dozunda progesteron (P4) uygulandı. 3. gün 25 μl, 1 × 105 cfu/rat dozunda E. coli intrauterin olarak uygulandı. Çalışma sonunda tüm hayvanların yaşamına etik kurallara uygun bir şekilde son verildikten sonra kan örnekleri alındı. Alınan kan örneklerinden akyuvar (WBC) düzeyi için hemogram analizi yapıldı. Kan örneklerinden serum ayrıştırılarak ticari kit ile TNF-beta analizi yapılmıştır.

Bulgular: WBC (p=0.0004) ve TNF-beta (p=0.027) kontrol grubuna göre endometritis grubunda istatistiksel olarak daha yüksekti.

Sonuç: Sonuç olarak, endometritiste TNF-beta’nın artış göstermesi genital sistemdeki inflamasyonlar için tanı markırı olabilir. Fakat ilgili markır ile kapsamlı çalışmalar yapılarak etkinliği daha iyi ortaya konulabilir.

  • Tambouret R. 17 - Gynecologic Infections. In: Kradin RL, editor. Diagnostic Pathology of Infectious Disease (Second Edition) [Internet]. Elsevier; 2018 [cited 2021 Jan 23]. p. 468–88. Available from: http://www.sciencedirect.com/science/article/pii/B9780323445856000175
  • Cohen CR, Manhart LE, Bukusi EA, Astete S, Brunham RC, Holmes KK, et al. Association between Mycoplasma genitalium and acute endometritis. The Lancet. 2002 Mar 2;359(9308):765–6.
  • Tulikangas PK, Schimpf MO. Chapter 22 - Genital and Urinary Tract Infections. In: Sokol AI, Sokol ER, editors. General Gynecology [Internet]. Philadelphia: Mosby; 2007 [cited 2021 Jan 23]. p. 523–42. Available from: http://www.sciencedirect.com/science/article/pii/B978032303247610022X
  • Ness R, Brooks-nelson D. 29 - Pelvic Inflammatory Disease. In: Goldman MB, Hatch MC, editors. Women and Health [Internet]. San Diego: Academic Press; 2000 [cited 2020 Aug 10]. p. 369–80. Available from: http://www.sciencedirect.com/science/article/pii/B9780122881459500334
  • Wiesenfeld H, Paavonen J. 6 - Pelvic Inflammatory Disease. In: Morse SA, Ballard RC, Holmes KK, Moreland AA, editors. Atlas of Sexually Transmitted Diseases and AIDS (Fourth Edition) [Internet]. London: W.B. Saunders; 2010 [cited 2020 Aug 10]. p. 94–110. Available from: http://www.sciencedirect.com/science/article/pii/B9780702040603000065
  • Kindelberger DW, Nucci MR. Chapter 6 - Benign Endometrium. In: Nucci MR, Oliva E, Goldblum JR, editors. Gynecologic Pathology [Internet]. Edinburgh: Churchill Livingstone; 2009 [cited 2021 Mar 3]. p. 197–232. Available from: https://www.sciencedirect.com/science/article/pii/B9780443069208500109
  • Şahin L, Gül HF, Dolanbay T, Makav M, Aras M. The Efficacy of Blood Parameters and Its Derivatives in the Diagnosis of Acute Cholecystitis. Medical Journal of Mugla Sitki Kocman University, 2020; 7(3), 135-140.
  • Ruddle NH. Lymphotoxin. In: Ratcliffe MJH, editor. Encyclopedia of Immunobiology [Internet]. Oxford: Academic Press; 2016 [cited 2021 Mar 3]. p. 466–75. Available from: https://www.sciencedirect.com/science/article/pii/B9780123742797100037
  • Cora MC, Kooistra L, Travlos G. Vaginal Cytology of the Laboratory Rat and Mouse: Review and Criteria for the Staging of the Estrous Cycle Using Stained Vaginal Smears. Toxicol Pathol. 2015 Aug 1;43(6):776–93. Peterson M, Dabbs DJ, Weidner N. CHAPTER 37 - Uterus. In: Weidner N, Cote RJ, Suster S, Weiss LM, editors. Modern Surgical Pathology (Second Edition) [Internet]. Philadelphia: W.B. Saunders; 2009 [cited 2021 Mar 4]. p. 1295–340. Available from: https://www.sciencedirect.com/science/article/pii/B9781416039662000370
  • Demirel MA, Han S, Tokmak A, Gokay NE, Uludag MO, Ustun TY, et al. Therapeutic effects of resveratrol in Escherichia coli-induced rat endometritis model. Naunyn Schmiedebergs Arch Pharmacol. 2019;392(12):1577–89.
  • Adnane M, Kaidi R, Hanzen C, England GCW. Risk factors of clinical and subclinical endometritis in cattle: a review. Turk J Vet Anim Sci. :11.
  • Salah N, Yimer N. Cytological endometritis and its agreement with ultrasound examination in postpartum beef cows. Vet World. 2017 Jun;10(6):605–9.
  • Sheldon IM, Dobson H. Postpartum uterine health in cattle. Anim Reprod Sci. 2004 Jul 1;82–83:295–306.
  • Wilson DA, editor. White Blood Cells. In: Clinical Veterinary Advisor [Internet]. Saint Louis: W.B. Saunders; 2012 [cited 2021 Mar 4]. p. 972. Available from: https://www.sciencedirect.com/science/article/pii/B9781416099796004554
  • Bolliger AP, Everds N. Chapter 2.9 - Haematology of the Mouse. In: Hedrich HJ, editor. The Laboratory Mouse (Second Edition) [Internet]. Boston: Academic Press; 2012 [cited 2021 Mar 4]. p. 331–47. Available from: https://www.sciencedirect.com/science/article/pii/B9780123820082000143
  • Celkan TT. Hemogram bize neler söyler? Turk Arch Pediatr. 2020;55(2).
  • Sauer M, Altrichter J, Kreutzer H-J, Schmidt H, Nöldge‐Schomburg G, Schmidt R, et al. Safety Evaluation for a Cell-based Immune Support System in an Ex Vivo Rat Model of Gram-negative Sepsis. Ther Apher Dial. 2009;13(5):444–50.
  • Hale A, Kirby JE, Albrecht M. Fatal Spontaneous Clostridium bifermentans Necrotizing Endometritis: A Case Report and Literature Review of the Pathogen. Open Forum Infect Dis [Internet]. 2016 Apr 1 [cited 2021 Mar 4];3(ofw095). Available from: https://doi.org/10.1093/ofid/ofw095
  • Heidarpour M, Mohri M, Fallah-Rad AH, Shahreza FD, Mohammadi M. Hematological changes before and after treatment in dairy cows with clinical and subclinical endometritis. 2014;5.
  • Nedwin GE, Naylor SL, Sakaguchi AY, Smith D, Jarrett-Nedwin J, Pennica D, et al. Human Lymphotoxin and tumor necrosis factor genes: structure, homology and chromosomal localization. Nucleic Acids Res. 1985 Sep 11;13(17):6361–73.
  • Temple SEL, Almeida CM, Cheong KY, Wunderink RG, Waterer GW. A diplotype in the lymphotoxin alpha gene is associated with differential expression of LTA mRNA induced by Gram-positive and Gram-negative bacteria. Int J Immunogenet. 2007;34(3):157–60.
  • Jin H, Uddin MS, Huang YL, Teo WK. Purification and renaturation of recombinant human lymphotoxin (tumour necrosis factor beta) expressed in Escherichia coli as inclusion bodies. J Chem Technol Biotechnol Oxf Oxfs 1986. 1994 Jan;59(1):67–72.
  • Buhrmann C, Shayan P, Aggarwal BB, Shakibaei M. Evidence that TNF-β (lymphotoxin α) can activate the inflammatory environment in human chondrocytes. Arthritis Res Ther. 2013 Nov 28;15(6):R202.
Birincil Dil en
Konular Toksikoloji
Bölüm Original Articles
Yazarlar

Orcid: 0000-0003-1879-8180
Yazar: Mustafa MAKAV (Sorumlu Yazar)
Kurum: KAFKAS ÜNİVERSİTESİ
Ülke: Turkey


Orcid: 0000-0003-4409-251X
Yazar: Mushap KURU
Kurum: KAFKAS ÜNİVERSİTESİ
Ülke: Turkey


Tarihler

Başvuru Tarihi : 8 Mart 2021
Kabul Tarihi : 23 Mart 2021
Yayımlanma Tarihi : 30 Mart 2021

Bibtex @araştırma makalesi { ejtox893215, journal = {Eurasian Journal of Toxicology}, issn = {}, eissn = {2667-8675}, address = {http://ejtoxicology.com/}, publisher = {Acil Tıp Uzmanları Derneği}, year = {2021}, volume = {3}, pages = {16 - 20}, doi = {10.51262/ejtox.893215}, title = {Determination of TNF-beta Marker in Intrauterine E. coli Induced Endometritis Model in Rats}, key = {cite}, author = {Makav, Mustafa and Kuru, Mushap} }
APA Makav, M , Kuru, M . (2021). Determination of TNF-beta Marker in Intrauterine E. coli Induced Endometritis Model in Rats . Eurasian Journal of Toxicology , 3 (1) , 16-20 . DOI: 10.51262/ejtox.893215
MLA Makav, M , Kuru, M . "Determination of TNF-beta Marker in Intrauterine E. coli Induced Endometritis Model in Rats" . Eurasian Journal of Toxicology 3 (2021 ): 16-20 <https://dergipark.org.tr/tr/pub/ejtox/issue/61223/893215>
Chicago Makav, M , Kuru, M . "Determination of TNF-beta Marker in Intrauterine E. coli Induced Endometritis Model in Rats". Eurasian Journal of Toxicology 3 (2021 ): 16-20
RIS TY - JOUR T1 - Determination of TNF-beta Marker in Intrauterine E. coli Induced Endometritis Model in Rats AU - Mustafa Makav , Mushap Kuru Y1 - 2021 PY - 2021 N1 - doi: 10.51262/ejtox.893215 DO - 10.51262/ejtox.893215 T2 - Eurasian Journal of Toxicology JF - Journal JO - JOR SP - 16 EP - 20 VL - 3 IS - 1 SN - -2667-8675 M3 - doi: 10.51262/ejtox.893215 UR - https://doi.org/10.51262/ejtox.893215 Y2 - 2021 ER -
EndNote %0 Eurasian Journal of Toxicology Determination of TNF-beta Marker in Intrauterine E. coli Induced Endometritis Model in Rats %A Mustafa Makav , Mushap Kuru %T Determination of TNF-beta Marker in Intrauterine E. coli Induced Endometritis Model in Rats %D 2021 %J Eurasian Journal of Toxicology %P -2667-8675 %V 3 %N 1 %R doi: 10.51262/ejtox.893215 %U 10.51262/ejtox.893215
ISNAD Makav, Mustafa , Kuru, Mushap . "Determination of TNF-beta Marker in Intrauterine E. coli Induced Endometritis Model in Rats". Eurasian Journal of Toxicology 3 / 1 (Mart 2021): 16-20 . https://doi.org/10.51262/ejtox.893215
AMA Makav M , Kuru M . Determination of TNF-beta Marker in Intrauterine E. coli Induced Endometritis Model in Rats. Eurasian Journal of Toxicology. 2021; 3(1): 16-20.
Vancouver Makav M , Kuru M . Determination of TNF-beta Marker in Intrauterine E. coli Induced Endometritis Model in Rats. Eurasian Journal of Toxicology. 2021; 3(1): 16-20.
IEEE M. Makav ve M. Kuru , "Determination of TNF-beta Marker in Intrauterine E. coli Induced Endometritis Model in Rats", Eurasian Journal of Toxicology, c. 3, sayı. 1, ss. 16-20, Mar. 2021, doi:10.51262/ejtox.893215