Determination of TNF-beta Marker in Intrauterine E. coli Induced Endometritis Model in Rats

Year 2021, Volume: 3 Issue: 1, 16 - 20, 30.03.2021

Mustafa Makav , Mushap Kuru

https://doi.org/10.51262/ejtox.893215

Cited By: 2

Abstract

Objectives: This study aims to investigate the importance of tumor necrosis factor (TNF)-beta marker in the endometritis model created by E. coli.

Materials and Methods: Rats were divided into control and endometritis groups with 7 rats in each group in the study. All animals were fed as ad libitum. No application was made to rats in the control group. Progesterone (P4) was administered subcutaneously to rats in the experimental group at a daily dose of 16 mg/kg between the 0th and 4th days of the study. E. coli was administered intrauterinely at 25 μl and 1 × 105 CFU/rat on the 3rd day. Blood samples were taken at the end of the study after the lives of all animals were terminated in accordance with ethical rules. Hemogram analysis was performed for white blood cell (WBC) levels from the blood samples taken. The TNF-beta analysis was performed with a commercial kit by separating serum from blood samples.

Results: WBC (p=0.0004) and TNF-beta (p=0.027) were statistically higher in the endometritis group compared to the control group.

Conclusion: Consequently, increased TNF-beta in endometritis may be a diagnostic marker for inflammations in the genital tract. However, its effectiveness may be better demonstrated by conducting comprehensive studies with the relevant marker.

Keywords

TNF-beta, WBC, Endometritis, Rat

References

• Tambouret R. 17 - Gynecologic Infections. In: Kradin RL, editor. Diagnostic Pathology of Infectious Disease (Second Edition) [Internet]. Elsevier; 2018 [cited 2021 Jan 23]. p. 468–88. Available from: http://www.sciencedirect.com/science/article/pii/B9780323445856000175
• Cohen CR, Manhart LE, Bukusi EA, Astete S, Brunham RC, Holmes KK, et al. Association between Mycoplasma genitalium and acute endometritis. The Lancet. 2002 Mar 2;359(9308):765–6.
• Tulikangas PK, Schimpf MO. Chapter 22 - Genital and Urinary Tract Infections. In: Sokol AI, Sokol ER, editors. General Gynecology [Internet]. Philadelphia: Mosby; 2007 [cited 2021 Jan 23]. p. 523–42. Available from: http://www.sciencedirect.com/science/article/pii/B978032303247610022X
• Ness R, Brooks-nelson D. 29 - Pelvic Inflammatory Disease. In: Goldman MB, Hatch MC, editors. Women and Health [Internet]. San Diego: Academic Press; 2000 [cited 2020 Aug 10]. p. 369–80. Available from: http://www.sciencedirect.com/science/article/pii/B9780122881459500334
• Wiesenfeld H, Paavonen J. 6 - Pelvic Inflammatory Disease. In: Morse SA, Ballard RC, Holmes KK, Moreland AA, editors. Atlas of Sexually Transmitted Diseases and AIDS (Fourth Edition) [Internet]. London: W.B. Saunders; 2010 [cited 2020 Aug 10]. p. 94–110. Available from: http://www.sciencedirect.com/science/article/pii/B9780702040603000065
• Kindelberger DW, Nucci MR. Chapter 6 - Benign Endometrium. In: Nucci MR, Oliva E, Goldblum JR, editors. Gynecologic Pathology [Internet]. Edinburgh: Churchill Livingstone; 2009 [cited 2021 Mar 3]. p. 197–232. Available from: https://www.sciencedirect.com/science/article/pii/B9780443069208500109
• Şahin L, Gül HF, Dolanbay T, Makav M, Aras M. The Efficacy of Blood Parameters and Its Derivatives in the Diagnosis of Acute Cholecystitis. Medical Journal of Mugla Sitki Kocman University, 2020; 7(3), 135-140.
• Ruddle NH. Lymphotoxin. In: Ratcliffe MJH, editor. Encyclopedia of Immunobiology [Internet]. Oxford: Academic Press; 2016 [cited 2021 Mar 3]. p. 466–75. Available from: https://www.sciencedirect.com/science/article/pii/B9780123742797100037
• Cora MC, Kooistra L, Travlos G. Vaginal Cytology of the Laboratory Rat and Mouse: Review and Criteria for the Staging of the Estrous Cycle Using Stained Vaginal Smears. Toxicol Pathol. 2015 Aug 1;43(6):776–93. Peterson M, Dabbs DJ, Weidner N. CHAPTER 37 - Uterus. In: Weidner N, Cote RJ, Suster S, Weiss LM, editors. Modern Surgical Pathology (Second Edition) [Internet]. Philadelphia: W.B. Saunders; 2009 [cited 2021 Mar 4]. p. 1295–340. Available from: https://www.sciencedirect.com/science/article/pii/B9781416039662000370
• Demirel MA, Han S, Tokmak A, Gokay NE, Uludag MO, Ustun TY, et al. Therapeutic effects of resveratrol in Escherichia coli-induced rat endometritis model. Naunyn Schmiedebergs Arch Pharmacol. 2019;392(12):1577–89.
• Adnane M, Kaidi R, Hanzen C, England GCW. Risk factors of clinical and subclinical endometritis in cattle: a review. Turk J Vet Anim Sci. :11.
• Salah N, Yimer N. Cytological endometritis and its agreement with ultrasound examination in postpartum beef cows. Vet World. 2017 Jun;10(6):605–9.
• Sheldon IM, Dobson H. Postpartum uterine health in cattle. Anim Reprod Sci. 2004 Jul 1;82–83:295–306.
• Wilson DA, editor. White Blood Cells. In: Clinical Veterinary Advisor [Internet]. Saint Louis: W.B. Saunders; 2012 [cited 2021 Mar 4]. p. 972. Available from: https://www.sciencedirect.com/science/article/pii/B9781416099796004554
• Bolliger AP, Everds N. Chapter 2.9 - Haematology of the Mouse. In: Hedrich HJ, editor. The Laboratory Mouse (Second Edition) [Internet]. Boston: Academic Press; 2012 [cited 2021 Mar 4]. p. 331–47. Available from: https://www.sciencedirect.com/science/article/pii/B9780123820082000143
• Celkan TT. Hemogram bize neler söyler? Turk Arch Pediatr. 2020;55(2).
• Sauer M, Altrichter J, Kreutzer H-J, Schmidt H, Nöldge‐Schomburg G, Schmidt R, et al. Safety Evaluation for a Cell-based Immune Support System in an Ex Vivo Rat Model of Gram-negative Sepsis. Ther Apher Dial. 2009;13(5):444–50.
• Hale A, Kirby JE, Albrecht M. Fatal Spontaneous Clostridium bifermentans Necrotizing Endometritis: A Case Report and Literature Review of the Pathogen. Open Forum Infect Dis [Internet]. 2016 Apr 1 [cited 2021 Mar 4];3(ofw095). Available from: https://doi.org/10.1093/ofid/ofw095
• Heidarpour M, Mohri M, Fallah-Rad AH, Shahreza FD, Mohammadi M. Hematological changes before and after treatment in dairy cows with clinical and subclinical endometritis. 2014;5.
• Nedwin GE, Naylor SL, Sakaguchi AY, Smith D, Jarrett-Nedwin J, Pennica D, et al. Human Lymphotoxin and tumor necrosis factor genes: structure, homology and chromosomal localization. Nucleic Acids Res. 1985 Sep 11;13(17):6361–73.
• Temple SEL, Almeida CM, Cheong KY, Wunderink RG, Waterer GW. A diplotype in the lymphotoxin alpha gene is associated with differential expression of LTA mRNA induced by Gram-positive and Gram-negative bacteria. Int J Immunogenet. 2007;34(3):157–60.
• Jin H, Uddin MS, Huang YL, Teo WK. Purification and renaturation of recombinant human lymphotoxin (tumour necrosis factor beta) expressed in Escherichia coli as inclusion bodies. J Chem Technol Biotechnol Oxf Oxfs 1986. 1994 Jan;59(1):67–72.
• Buhrmann C, Shayan P, Aggarwal BB, Shakibaei M. Evidence that TNF-β (lymphotoxin α) can activate the inflammatory environment in human chondrocytes. Arthritis Res Ther. 2013 Nov 28;15(6):R202.