Determination of Resistance to Tomato Yellow Leaf Curl Virus by Molecular Methods in Pink Beef Tomatoes

Yıl 2024, Cilt: 10 Sayı: 1, 52 - 58, 01.02.2024

Mine Bulut Sevinç Tekin Elif Ürün Köksalan Veysel Aras

Öz

In both public and private tomato breeding projects, marker assisted selection (MAS) for disease resistance is frequently
used. In tomato molecular breeding programs, the development and application of molecular markers have been
extensively pursued, particularly for disease resistance to enable the selection of a single resistance gene or a combination
of multiple resistance genes. Tomato Yellow Leaf Curl Virus (TYLCV) is one of the most dangerous viruses affecting
tomato production and growth worldwide. Using resistant cultivars is the most effective and eco-friendly way to combat
TYLCV. In this study, the TYLCV was tested against 155 genotypes of pink beef tomatoes utilizing the MAS (MarkerAssisted Selection) technique. Resistance against TYLCV was determined with the SCAR (P6-25) primer developed in
connection with the Ty-3 gene. 42 pink tomato genotypes were determined to be sensitive (rr), 99 to be heterozygous
resistant (Rr), and 8 to be homozygous resistant (RR) to TYLCV as a consequence of MAS testing. Furthermore, no
molecular marker was found in any of the six pink beef tomato genotypes. These findings suggested that P6-25 (SCAR)
primers could be used successfully in breeding studies to identify disease resistance.

Anahtar Kelimeler

Tomato, MAS, TYLCV, resistance breeding

Kaynakça

• Aktaş H, Aydın G (2022). Determination of The Response of Wild and Cultivated Tomato Genotypes to Some Disease and Pests by Molecular Markers, HortiS (2022) 39(1):15-21, https://doi.org/10.16882/HortiS.1069414
• Agnihotri MK, Gothwal RK, Moranıya N, Singh R, Verma S (2013). Breeding for Resistance to Tomato Yellow Leaf Curl Virus (TYLCV)/ Tomato Leaf Curl Virus (ToLCV), Agriculture for Sustainable Development, 1(1), 113-117.
• Agrama HA, Scott JW (2006). Quantitative Trait Loci for Tomato Yellow Leaf Curl Virus and Tomato Mottle Virus Resistance in Tomato. Journal of the American Society for Horticultural Science, 131:267-272.
• Bhandari HR, Srivastava K, Eswar Reddy G (2017). Genetic Variability, Heritability and Genetic Advance for Yield Traits in Tomato (Solanum lycopersicum L.). International Journal of Current Microbiology and Applied Sciences.;6(7):4131-4138. doi:10.20546/ ijcmas.2017.607.428
• Basım H, Kandil O, Karaoğlan M (2023). Determination of TYLCV-Resistant Cherry and Cocktail Tomato Cultivars by Molecular Markers, Turkish Journal of Science and Engineering, 5(2): 89-96, doi: 10.55979/tjse.1357477
• Caro M, Verlaan MG, Julián O, Finkers R, Wolters A-MA., Hutton SF, Scott JW, Kormelink R, Visser RGF, Diez MJ, Perez-de-Castro A, Bai Y (2015). Assessing the genetic variation of Ty-1 and Ty-3 alleles conferring resistance to tomato yellow leaf curl virus in a broad tomato germplasm. Molecular Breeding, 35(6). doi:10.1007/s11032-015-0329-y
• Cohen S, Lapidot M (2007). Appearance and Expansion of TYLCV: a Historical Point of View. In: Czosnek H (ed) Tomato Yellow Leaf Curl Virus Disease: Management, Molecular Biology, Breeding for Resistance. Springer, The Netherlands, pp 3–12. doi:10.1007/978-1-4020-4769-5
• Doyle JJ, Doyle JL (1990). Isolation of Plant DNA from Fresh Tissue, Focus, 12(1), 13-15. FAO (2022). Food and Agriculture Organization, Agricultural Statistical Database. https://www. fao.org/faostat/en/#data (accessed on 16 Januar 2024).
• Foolad MR (2007). Genome Mapping and Molecular Breeding of Tomato. International Journal of Plant Genomics, 2007, 1–52. doi:10.1155/2007/64358
• Gill U, Scott JW, Shekasteband R, Ogundiwin E, Schuit C, Francis DM, Sim SC, Smith H, Hutton SF (2019). Ty-6, a major begomovirus resistance gene on chromosome 10, is effective against Tomato yellow leaf curl virus and Tomato mottle virus. Theoretical and Applied Genetics, 132, 1543-1554, doi: 10.1007/s00122-019-03298-0
• Glick E, Levy Y, Gafni Y (2009). The Viral Etiology of Tomato Yellow Leaf Curl Disease – a Review. Plant Protect. Sci., 45: 81–97.
• Ji Y, Schuster DJ, Scott JW (2007). Ty-3, a Begomovirus Resistance Locus Near The Tomato Yellow Leaf Curl Virus Resistance Locus Ty-1 on Chromosome 6 of Tomato, Mol. Breed., 20, 271–284. doi:10.1007/S11032-007-9089-7
• Ji Y, Scott JW, Schuster DJ (2009a). Toward Fine Mapping of The Tomato Yellow Leaf Curl Virus Resistance Gene Ty-2 on Chromosome 11 of Tomato. HortScience, 44, 614-618, https://doi. org/10.21273/HORTSCI.44.3.614
• Ji Y, Scott JW, Schuster DJ, Maxwell DP (2009b). Molecular Mapping of Ty-4, a New Tomato Yellow Leaf Curl Virus Resistance Locus on Chromosome 3 of Tomato. Journal of the American Society for Horticultural Science, 134, 281-288, https://doi.org/10.21273/ JASHS.134.2.281
• Jones JB, Jones JP, Stall RE, Zitter TA (1991). Compendium of Tomato Diseases. American Phytopathological Society Press, St.Paul.
• Jung, J., Kim, H. J., Lee, J. M., Oh, C. S., Lee, H.- J., & Yeam, I. (2015). Gene-based molecular marker system for multiple disease resistances in tomato against Tomato yellow leaf curl virus, late blight, and verticillium wilt. Euphytica, 205(2), 599–613. doi:10.1007/s10681-015-1442-z
• Kim M, Park Y, Lee J, Sim SC (2020). Development of molecular markers for Ty-2 and Ty-3 selection in tomato breeding. Scientia Horticulturae, 265, 109230. doi:10.1016/j.scienta.2020.109230
• Knapp S, Hawkes JG, Lester RN, Nee M, EstradaRamos N (1992). Solanaceae III: Taxonomy, Chemistry, Evolution. Kew Bulletin, 47(4), 785. doi:10.2307/4110730
• Knapp S, Bohs L, Nee M, Spooner DM (2004). Solanaceae—A Model for Linking Genomics with Biodiversity. Comparative and Functional Genomics, 5(3), 285–291. doi:10.1002/cfg.393
• Lapidot M, Karniel U, Gelbart D, Fogel D, Evenor D, Kutsher Y, Makhbash Z, Nahon S, Shlomo H, Chen L, Reuveni M, Levin I (2015). A Novel route controlling begomovirus resistance by the messenger RNA surveillance factor pelota. PLOS Genetics, 11(10), e1005538. doi:10.1371/journal. pgen.1005538
• Laterrot H, (1995). Breeding network to create tomato varieties resistant to tomato yellow leaf crul virus (TYLCV), Fruits, 50:439–444.
• Melomey LD, Danquah A, Offei SK, Ofori K, Danquah E and Osei M, (2019). Review on tomato (Solanum lycopersicum, L.) improvement programmes in Ghana. Recent advances in tomato breeding and production, 49.
• Moriones E, Navas-Castillo J, (2000). Tomato yellow leaf curl virus, an emerging virus complex causing epidemics worldwide. Virus Research, 71:123-34, https://doi.org/10.1016/S0168-1702(00)00193-3
• Ogunsola OA, Ogunsina GA, (2021). Tomato production and associated stress: A case of african climate single cell biology, Vol.10 Iss.4 No:1000003.
• Pınar H, Atilla A, Keleş D, Mutlu N, Denli N, Mustafa Ü, (2013). Determination of Fusarium oxysporum f. sp. lycopersici resistance in tomato lines with the help of molecular markers. Derim, 30(1):15-23.
• Prasanna HC, Sinha DP, Rai GK, Krishna R, Kashyap SP, Singh NK, Singh M, Malathi V G, (2014).
• Pyramiding Ty-2 and Ty-3 genes for resistance to monopartite and bipartite tomato leaf curl viruses of India. Plant Pathology, 64(2): 256-264. doi:10.1111/ppa.12267
• Polston JE, Anderson PK, (1997). The Emergence of whitefly-transmitted geminiviruses in tomato in the western hemisphere, Plant Dis., 81, 1358- 1369, doi: 10.1094/PDIS.1997.81.12.1358
• Robertson LD, Labate JA, (2007). Genetic resources of tomato (Lycopersicum esculentum Mill) and wild relatives. In: Razdan MK, Mattoo AK, editors.
• Genetic Improvement of Solanaceous Crops. Vol. 2. Tomato. New Hampshire: Science Publishers. pp. 25-75
• Yang X, Caro M, Hutton SF, Scott JW, Guo Y, Wang X, Rashid MH, Szinay D, de Jong H, Visser RG, Bai Y, Du Y, (2014). Fine mapping of the tomato yellow leaf curl virus resistance gene Ty-2 on chromosome 11 of tomato. Molecular Breeding. doi:10.1007/s11032-014-0072-9
• Zamir D, Ekstein-Michelson I, Zakay Y, Navot N, Zeidan M, Sarfatti M, Eshed Y, Harel E, Pleben H, VanOss H, Kedar N, Rabinowitch HD, Czosnek H, (1994). Mapping and introgression of tomato yellow leaf curl virus tolerance gene, ty-1, theor. Appl. Genet., 88:141-146.