Sıvı Kültür Ortamında Büyüme Düzenleyicilerinin Rotala rotundifolia (Buch-Ham. Ex Roxb)’nın Hızlı Klonal Çoğaltımı Üzerine Etkisi

Abstract

Rotala rotundifolia (Buch-Ham. ex Roxb) tıbbi ve süs amaçlı kullanılan önemli bir su bitkisidir. Bu çalışmanın amacı farklı büyüme düzenleyicilerinin R. rotundifolia’nın in vitro klonal çoğaltımı üzerine farklı büyüme düzenleyicilerinin etkilerini araştırmaktır. Tüm dememeler sıvı Murashige ve Skoog (MS) ortamında yürütülmüştür (agarsız). Boğum eksplantları sıvı besin ortamlarına aktarılmıştır. Besin solüsyonlarına büyüme düzenleyici olarak farklı kombinasyonlarda Gibberellik asit (GA3) + Thidiazuron (TDZ) ve Kinetin (KIN) + Gibberellik asit (GA3) eklenmiştir. Sürgün sayısı ve uzunlukları açısından en iyi sonuçlar 0.25 mg/L GA3 + 0.25 mg/L TDZ ve 0.25 mg/L KIN+ 0.25 mg/L GA3 içeren besin solüsyonunda belirlenmiştir. Her iki hormon kombinasyonları karşılaştırıldığında, daha fazla sürgün sayıları GA3+TDZ içeren besin solüsyonlarında bulunmuştur. Buna karşın daha uzun sürgünler KIN+GA3 içeren besin solüsyonlarında elde edilmiştir. Çoğaltım denemelerinden sonra bitkiler 0.25 mg/L indol-3-bütirik asit (IBA)’lı ortamda kök oluşturdu. Üretimi tamamlanan bitkiler akvaryuma başarıyla adapte edilmiştir.

Keywords

• in vitro çoğaltım
• tıbbi bitki
• MS medium
• boğum eksplant
• doku kültürü

Impact of Growth Regulators on Rapid Clonal Propagation of Rotala rotundifolia (Buch-Ham. ex Roxb) in Liquid Culture Medium

Abstract

Rotala rotundifolia (Buch-Ham. ex Roxb) is water plant used for medicinal and ornamental purposes. The aim of the work was to investigate the impacts of different growth regulators on the in vitro clonal propagation of R. rotundifolia. All assays were performed in liquid Murashige and Skoog (MS) media (without agar). Nodal explants were transferred to liquid MS nutrient media. Different combinations of Gibberellic Acid (GA3) + Thidiazuron (TDZ) and Kinetin (KIN) + Gibberellic Acid (GA3) were added to the nutrient solutions as growth regulators. The best results in terms of shoot numbers and shoot lengths were determined in nutrient solution including 0.25 mg/L GA3+ 0.25 mg/L TDZ and 0.25 mg/L KIN+ 0.25 mg/L GA3. When both hormone combinations were compared, higher shoot numbers were found in nutrient solutions including GA3+TDZ. On the other hand, longer shoots were obtained in nutrient solutions including KIN+GA3 After the propagation trials, the plants formed roots in the media with 0.25 mg/L indole-3-butyric acid (IBA). The plants that have been produced have been successfully adapted to the aquarium.

Keywords

• in vitro propagation
• medicinal plant
• MS medium
• nodal explant
• tissue culture

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