Metformin And Dichloroacetate Combination Exert A Synergistic Effect On Cell Viability Of Oral Squamous Cell Carcinoma

Yıl 2019, Cilt: 9 Sayı: 2, 68 - 73, 08.08.2019

Seniz Inanc Didem Keles Gorkem Eskiizmir Yasemin Basbinar Gulgun Oktay

https://doi.org/10.32448/entupdates.569464

Öz

Objective: To assess the effects of Metformin, Dichloroacetate (DCA) and their combination on cell viability in oral squamous cell carcinoma, UPCI-SCC-131 cell line.

Methods: UPCI-SCC-131 cells were plated in 96 E-plate (1x104 cells/well) and were treated with Metformin (1-16mM) and/or DCA (15-120mM) for 24-48-72h. xCELLigence SP system was used to monitor real time cell viability. In addition, drug combination index was analyzed with CompuSyn software according to Chou-Talalay method.

Results: Half-maximal inhibitory concentrations (IC50) of Metformin and DCA were found to be 3mM and 23mM, respectively, for 72 hours. CI values (0.76-0.80) in all combination groups below 1 indicated that Metformin/DCA combination had a moderate synergistic effect on cell viability in UPCI-SCC-131 cells.

Discussion: Metformin/DCA combination synergistically decreased the cell viability of UPCI-SCC-131 cells. Therefore, a combined application of Metformin and DCA may be considered as a candidate therapy for the “drug repositioning” of the treatment of oral cavity cancer.

Anahtar Kelimeler

Metformin , Dichloroacetate , Drug combination , Oral Cavity Cancer

Destekleyen Kurum

Dokuz Eylul University Scientific Research Project Coordination Unit

Proje Numarası

2017.KB.SAĞ.001

Kaynakça

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