Yıl 2019, Cilt 7 , Sayı , Sayfalar 265 - 274 2019-11-24

Biochemical Study of Dipeptidyl Peptidase-4 in Autistic's Patients

Safaa A. AL-AMEEN [1] , Fadwa Kh. TAWFEEQ [2] , Tareq Y. AHMAD [3]


The research includes estimation of autistics' serum dipeptidyl peptidase-4 (DPP-4) activity. The results indicated a significant (p≤0.05) decrease in (DPP-4) activity of autism spectrum disorder groups, 57.85, 42.86, 36.47 µmol/L respectively compared to control group. By statistical analysis, the study revealed a significant (p≤0.05) relationship between DPP-4 activity with gastrointestinal disorder on one hand and with various inflammation incidence on the other hand. Partial purification of DPP-4 from serum of normal person age 14 years in Mosul city was done. Gel filtration of dialysate precipitate produced by 50% ammonium sulphate saturation has given two major proteinous components. One of them ( peak A) possesses a high DPP-4 activity using sephedex G-100. The apparent molecular weight of the isolated DPP-4 was 176.6 KD. Then SDS-PAGE was performed. HPLC revealed a single peak A' at retention time 5.829 min by application the top of peak A which was isolated from gel filtration. Maximum activity of DPP-4 was obtained using 0.1 M Tris-HCl buffer at pH 8, 40°C, 4 mM of gly-pro-p-nitroanilide hydrochloride as a substrate. Maximum velocity (Vmax) was 50 μM according to Line Weaver-Burk plot while Michaelis–Menten constant (Km) was 0.5 mM. Mercuric chloride and strontium chloride hexahydrate at 5 mM revealed maximum inhibitory effect of DPP4 activity by 30.2% and 42.9% respectively.
Dipeptidyl peptidase 4, Autism spectrum disorder, Gel filtration, Electrophoresis, High performance liquid chromatography
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Birincil Dil en
Konular Mühendislik
Bölüm Makaleler
Yazarlar

Yazar: Safaa A. AL-AMEEN
Kurum: University of Mosul
Ülke: Iraq


Yazar: Fadwa Kh. TAWFEEQ
Kurum: University of Mosul
Ülke: Iraq


Yazar: Tareq Y. AHMAD
Kurum: University of Mosul
Ülke: Iraq


Tarihler

Yayımlanma Tarihi : 24 Kasım 2019

Bibtex @araştırma makalesi { epstem656403, journal = {The Eurasia Proceedings of Science Technology Engineering and Mathematics}, issn = {}, eissn = {2602-3199}, address = {isresoffice@gmail.com}, publisher = {ISRES Organizasyon Turizm Eğitim Danışmanlık Ltd. Şti.}, year = {2019}, volume = {7}, pages = {265 - 274}, doi = {}, title = {Biochemical Study of Dipeptidyl Peptidase-4 in Autistic's Patients}, key = {cite}, author = {AL-AMEEN, Safaa A. and TAWFEEQ, Fadwa Kh. and AHMAD, Tareq Y.} }
APA AL-AMEEN, S , TAWFEEQ, F , AHMAD, T . (2019). Biochemical Study of Dipeptidyl Peptidase-4 in Autistic's Patients. The Eurasia Proceedings of Science Technology Engineering and Mathematics , 7 () , 265-274 . Retrieved from https://dergipark.org.tr/tr/pub/epstem/issue/50288/656403
MLA AL-AMEEN, S , TAWFEEQ, F , AHMAD, T . "Biochemical Study of Dipeptidyl Peptidase-4 in Autistic's Patients". The Eurasia Proceedings of Science Technology Engineering and Mathematics 7 (2019 ): 265-274 <https://dergipark.org.tr/tr/pub/epstem/issue/50288/656403>
Chicago AL-AMEEN, S , TAWFEEQ, F , AHMAD, T . "Biochemical Study of Dipeptidyl Peptidase-4 in Autistic's Patients". The Eurasia Proceedings of Science Technology Engineering and Mathematics 7 (2019 ): 265-274
RIS TY - JOUR T1 - Biochemical Study of Dipeptidyl Peptidase-4 in Autistic's Patients AU - Safaa A. AL-AMEEN , Fadwa Kh. TAWFEEQ , Tareq Y. AHMAD Y1 - 2019 PY - 2019 N1 - DO - T2 - The Eurasia Proceedings of Science Technology Engineering and Mathematics JF - Journal JO - JOR SP - 265 EP - 274 VL - 7 IS - SN - -2602-3199 M3 - UR - Y2 - 2020 ER -
EndNote %0 The Eurasia Proceedings of Science Technology Engineering and Mathematics Biochemical Study of Dipeptidyl Peptidase-4 in Autistic's Patients %A Safaa A. AL-AMEEN , Fadwa Kh. TAWFEEQ , Tareq Y. AHMAD %T Biochemical Study of Dipeptidyl Peptidase-4 in Autistic's Patients %D 2019 %J The Eurasia Proceedings of Science Technology Engineering and Mathematics %P -2602-3199 %V 7 %N %R %U
ISNAD AL-AMEEN, Safaa A. , TAWFEEQ, Fadwa Kh. , AHMAD, Tareq Y. . "Biochemical Study of Dipeptidyl Peptidase-4 in Autistic's Patients". The Eurasia Proceedings of Science Technology Engineering and Mathematics 7 / (Kasım 2019): 265-274 .
AMA AL-AMEEN S , TAWFEEQ F , AHMAD T . Biochemical Study of Dipeptidyl Peptidase-4 in Autistic's Patients. EPSTEM. 2019; 7: 265-274.
Vancouver AL-AMEEN S , TAWFEEQ F , AHMAD T . Biochemical Study of Dipeptidyl Peptidase-4 in Autistic's Patients. The Eurasia Proceedings of Science Technology Engineering and Mathematics. 2019; 7: 274-265.