Araştırma Makalesi
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Macroscopic Evaluation of the Effect of Topically Applied Tideglusib with Bacterial Cellulose-Based Hydrogel Carrier on Healing of Palatal Wound in Rats: A Preliminary Study

Yıl 2023, , 72 - 78, 31.08.2023
https://doi.org/10.29228/erd.48

Öz

Objectives: The aim of this study is to macroscopically evaluate the early effect of topically applied bacterial cellulose (BC) based hydrogel carrier including tideglusib (Td) on palatal wound in rats.
Materials and Methods: The study group consisted of 3 months old male, 12 Sprague Dawley rats, each weighing an average of 250-300 gr. Three animals were instantly sacrificed and represented the baseline group at time 0 (initial group). The remaining nine were divided into three groups at random: empty group, hydrogel group, Td+hydrogel group. Mean wound surface area was measured photographically.
Results: In the intergroup comparison, a statistically significant difference was observed in the wound surface area (p=0.033). In the pairwise comparisons between the groups, a statistically significant difference was found only between the initial group and the Td+Hydrogel group (p=0,028).
Conclusions: Td topically applied with BC based hydrogel has positive effects on palatal mucosal wound healing of rats.

Proje Numarası

TDK-2022-10366

Kaynakça

  • Abbenhaus JI, MacMahon RA, Rosenkrantz JG, Paton BC. Collagen sheets as a dressing for large excised areas. Surg Forum. 1965;16: 477-478.
  • Abdelraof M, Hasanin MS, Farag MM, Ahmed HY. Green synthesis of bacterial cellulose/bioactive glass nanocomposites: Effect of glass nanoparticles on cellulose yield, biocompatibility and antimicrobial activity. Int J Biol Macromol. 2019;138: 975-985.
  • Adamowicz K, Wang H, Jotwani R, Zeller I, Potempa J, Scott DA. Inhibition of GSK3 abolishes bacterial-induced periodontal bone loss in mice. Mol Med. 2012;18: 1190-1196.
  • Ahmed S, Ikram S. Chitosan and gelatin based biodegradable packaging films with UV-light protection. J Photochem Photobiol B. 2016;163: 115-124.
  • Ayvazyan A, Morimoto N, Kanda N, Takemoto S, Kawai K, Sakamoto Y, Taira T, Suzuki S. Collagen-gelatin scaffold impregnated with bFGF accelerates palatal wound healing of palatal mucosa in dogs. J Surg Res. 2011;171(2): e247-257.
  • Bhola M, Newell DH, Hancock EB. Acellular dermal allograft for vestibuloplasty--an alternative to autogenous soft tissue grafts in preprosthetic surgical procedures: a clinical report. J Prosthodont. 2003;12(2): 133-137.
  • Chandarana SP, Chanowski EJ, Casper KA, Wolf GT, Bradford CR, Worden FP, Eisbruch A, Chepeha DB. Osteocutaneous free tissue transplantation for mandibular osteoradionecrosis. J Reconstr Microsurg. 2013;29(1): 5-14.
  • Cortellini P, Pini Prato G, Tonetti MS. Interproximal free gingival grafts after membrane removal in guided tissue regeneration treatment of intrabony defects. A randomized controlled clinical trial. J Periodontol. 1995;66(6): 488-493.
  • Deng P, Yao L, Chen J, Tang Z, Zhou J. Chitosan-based hydrogels with injectable, self-healing and antibacterial properties for wound healing. Carbohydr Polym. 2022;276: 118718.
  • Duskova M, Leamerova E, Sosna B, Gojis O. Guided tissue regeneration, barrier membranes and reconstruction of the cleft maxillary alveolus. J Craniofac Surg. 2006;17(6): 1153-1160.
  • Eisenmann DM. Wnt signaling. WormBook. 2005: 1-17.
  • Evans EW. Treating Scars on the Oral Mucosa. Facial Plast Surg Clin North Am. 2017;25(1): 89-97.
  • Fowler EB, Breault LG, Rebitski G. Ridge preservation utilizing an acellular dermal allograft and demineralized freeze-dried bone allograft: Part II. Immediate endosseous implant placement. J Periodontol. 2000;71(8): 1360-1364.
  • Francesko A, Tzanov T. Chitin, chitosan and derivatives for wound healing and tissue engineering. Adv Biochem Eng Biotechnol. 2011;125: 1-27.
  • Guler R, Ercan MT, Ulutuncel N, Devrim H, Uran N. Measurement of blood flow by the 133Xe clearance technique to grafts of amnion used in vestibuloplasty. Br J Oral Maxillofac Surg. 1997;35(4): 280-283.
  • Hakkinen L, Uitto VJ, Larjava H. Cell biology of gingival wound healing. Periodontol 2000. 2000;24: 127-152.
  • Hammad HM, Hammad MM, Abdelhadi IN, Khalifeh MS. Effects of topically applied agents on intra-oral wound healing in a rat model: a clinical and histomorphometric study. Int J Dent Hyg. 2011;9(1): 9-16.
  • Ito M, Yang Z, Andl T, Cui C, Kim N, Millar SE, Cotsarelis G. Wnt-dependent de novo hair follicle regeneration in adult mouse skin after wounding. Nature. 2007;447(7142): 316-320.
  • Jones V, Grey JE, Harding KG. Wound dressings. BMJ. 2006;332(7544): 777-780.
  • Kahnberg KE, Thilander H. Healing of experimental excisional wounds in the rat palate. II. Histological study of electrosurgical wounds. Swed Dent J. 1984;8(2): 49-56.
  • Kozlovsky A, Artzi Z, Hirshberg A, Israeli-Tobias C, Reich L. Effect of local antimicrobial agents on excisional palatal wound healing: a clinical and histomorphometric study in rats. J Clin Periodontol. 2007;34(2): 164-171.
  • Lee HG, Eun HC. Differences between fibroblasts cultured from oral mucosa and normal skin: implication to wound healing. J Dermatol Sci. 1999;21(3): 176-182.
  • Lektemur Alpan A, Calisir M, Kizildag A, Ozdede M, Ozmen O. Effects of a Glycogen Synthase Kinase 3 Inhibitor Tideglusib on Bone Regeneration With Calvarial Defects. J Craniofac Surg. 2020;31(5): 1477-1482.
  • May SR. The effects of biological wound dressings on the healing process. Clin Mater. 1991;8(3-4): 243-249.
  • Minear S, Leucht P, Jiang J, Liu B, Zeng A, Fuerer C, Nusse R, Helms JA. Wnt proteins promote bone regeneration. Sci Transl Med. 2010;2(29): 29ra30.
  • Nakayama A, Kakugo A, Gong JP, Osada Y, Takai M, Erata T. High mechanical strength double-network hydrogel with bacterial cellulose. Advanced Functional Materials. 2004;14(11): 1124-1128.
  • Narang S, Gupta R. Evaluation of resorbable membrane in treatment of human gingival isolated buccal recession. Indian J Dent Res. 2011;22(6): 749-754.
  • Neves VC, Babb R, Chandrasekaran D, Sharpe PT. Promotion of natural tooth repair by small molecule GSK3 antagonists. Sci Rep. 2017;7: 39654.
  • Noh YK, Dos Santos Da Costa A, Park YS, Du P, Kim IH, Park K. Fabrication of bacterial cellulose-collagen composite scaffolds and their osteogenic effect on human mesenchymal stem cells. Carbohydr Polym. 2019;219: 210-218.
  • Oncu B., Yılmaz A.M., Yılmaz B.K., Altunok E.Ç., Kuru L., Ö.B. A. Cytotoxicity and Collagen Expression Effects of Tideglusib Administration on Human Periodontal Cells: An In-Vitro Study. Clinical and Experimental Health Sciences. 2020;10: 153-162.
  • Popelut A, Rooker SM, Leucht P, Medio M, Brunski JB, Helms JA. The acceleration of implant osseointegration by liposomal Wnt3a. Biomaterials. 2010;31(35): 9173-9181.
  • Rastogi S, Modi M, Sathian B. The efficacy of collagen membrane as a biodegradable wound dressing material for surgical defects of oral mucosa: a prospective study. J Oral Maxillofac Surg. 2009;67(8): 1600-1606.
  • Ross P, Mayer R, Benziman M. Cellulose biosynthesis and function in bacteria. Microbiol Rev. 1991;55(1): 35-58.
  • Shanmugam M, Kumar TS, Arun KV, Arun R, Karthik SJ. Clinical and histological evaluation of two dressing materials in the healing of palatal wounds. J Indian Soc Periodontol. 2010;14(4): 241-244.
  • Speroni S, Cicciu M, Maridati P, Grossi GB, Maiorana C. Clinical investigation of mucosal thickness stability after soft tissue grafting around implants: a 3-year retrospective study. Indian J Dent Res. 2010;21(4): 474-479.
  • Tal H. Subgingival acellular dermal matrix allograft for the treatment of gingival recession: a case report. J Periodontol. 1999;70(9): 1118-1124.
  • Tanır HG, Demirezen Ş. Wnt Sinyal Yolunun Biyolojisi ve Bu Yolda Görev Alan Biyomoleküller. Turkiye Klinikleri Journal of Medical Sciences. 2009;29(5): 1292-1297.
  • Taskan MM, Balci Yuce H, Karatas O, Gevrek F, Isiker Kara G, Celt M, Sirma Taskan E. Hyaluronic acid with antioxidants improve wound healing in rats. Biotech Histochem. 2021;96(7): 536-545.
  • UI-Islam M, Shah N, Ha JH, Park J. Effect of chitosan penetration on physico-chemical and mechanical properties of bacterial cellulose. The Korean Journal of Chemical Engineering. 2011;28(8): 1736.
  • Waldrop TC, Semba SE. Closure of oroantral communication using guided tissue regeneration and an absorbable gelatin membrane. J Periodontol. 1993;64(11): 1061-1066.
  • Whyte JL, Smith AA, Helms JA. Wnt signaling and injury repair. Cold Spring Harb Perspect Biol. 2012;4(8): a008078.
  • Zaborowska M, Bodin A, Backdahl H, Popp J, Goldstein A, Gatenholm P. Microporous bacterial cellulose as a potential scaffold for bone regeneration. Acta Biomater. 2010;6(7): 2540-2547.
  • Zhao J, Kim KA, De Vera J, Palencia S, Wagle M, Abo A. R-Spondin1 protects mice from chemotherapy or radiation-induced oral mucositis through the canonical Wnt/beta-catenin pathway. Proc Natl Acad Sci U S A. 2009;106(7): 2331-2336.

Bakteriyel Selüloz Tabanlı Hidrojel Taşıyıcı ile Topikal Uygulanan Tideglusib’in Sıçanlarda Oluşturulan Palatinal Yaranın İyileşmesi Üzerine Etkisinin Makroskobik Değerlendirmesi: Bir Öncül Çalışma

Yıl 2023, , 72 - 78, 31.08.2023
https://doi.org/10.29228/erd.48

Öz

Amaç: Bu çalışmanın amacı, bakteriyel selüloz (BS) tabanlı hidrojel taşıyıcı ile topikal olarak uygulanan tideglusib (Td)'nin sıçanlarda oluşturulan palatinal yaranın iyileşmesi üzerindeki erken etkisini makroskobik olarak değerlendirmektir.
Gereç ve Yöntemler: Bu çalışmada deney hayvanı olarak; ortalama 3 aylık, ağırlıkları 250-300 gr arasında değişen, 12 adet genç erişkin erkek Sprague Dawley sıçan kullanıldı. Sıçanların palatinal mukozalarının ortasında 4 mm çapında mukozal defekt oluşturuldu. Üç hayvan, defekt oluşturulduktan hemen sonra sakrifiye edildi ve başlangıç grubunu oluşturdu. Diğer 9 hayvan, pasif kontrol, sadece taşıyıcı uygulanan (hidrojel) ve taşıyıcı içinde Td uygulanan (Td+Hidrojel) olmak üzere rastgele üç gruba ayrıldı. Bu üç gruptaki hayvanlar 7. günde sakrifiye edildi. Ortalama yara yüzey alanı fotoğraf üzerinden ölçülerek makroskobik olarak değerlendirildi.
Bulgular: Gruplar arası karşılaştırmada yara yüzey alanında istatistiksel olarak anlamlı farklılık gözlendi (p=0,033). Gruplar arası ikili karşılaştırmalarda sadece başlangıç grubu ile Td+Hidrojel grubu arasında istatistiksel olarak anlamlı bir fark bulundu (p=0,028).
Sonuç: BS tabanlı hidrojel ile topikal olarak uygulanan Td’nin, sıçanların palatal mukozal yara iyileşmesi üzerinde olumlu etkilere sahip olduğu makroskobik olarak gösterilmiştir.

Destekleyen Kurum

Marmara Üniversitesi Bilimsel Araştırma Projeleri Komisyonu Başkanlığı

Proje Numarası

TDK-2022-10366

Teşekkür

Bu çalışma, Marmara Üniversitesi Bilimsel Araştırma Projeleri Komisyonu Başkanlığı tarafından TDK-2022-10366 numaralı proje ile desteklendi.

Kaynakça

  • Abbenhaus JI, MacMahon RA, Rosenkrantz JG, Paton BC. Collagen sheets as a dressing for large excised areas. Surg Forum. 1965;16: 477-478.
  • Abdelraof M, Hasanin MS, Farag MM, Ahmed HY. Green synthesis of bacterial cellulose/bioactive glass nanocomposites: Effect of glass nanoparticles on cellulose yield, biocompatibility and antimicrobial activity. Int J Biol Macromol. 2019;138: 975-985.
  • Adamowicz K, Wang H, Jotwani R, Zeller I, Potempa J, Scott DA. Inhibition of GSK3 abolishes bacterial-induced periodontal bone loss in mice. Mol Med. 2012;18: 1190-1196.
  • Ahmed S, Ikram S. Chitosan and gelatin based biodegradable packaging films with UV-light protection. J Photochem Photobiol B. 2016;163: 115-124.
  • Ayvazyan A, Morimoto N, Kanda N, Takemoto S, Kawai K, Sakamoto Y, Taira T, Suzuki S. Collagen-gelatin scaffold impregnated with bFGF accelerates palatal wound healing of palatal mucosa in dogs. J Surg Res. 2011;171(2): e247-257.
  • Bhola M, Newell DH, Hancock EB. Acellular dermal allograft for vestibuloplasty--an alternative to autogenous soft tissue grafts in preprosthetic surgical procedures: a clinical report. J Prosthodont. 2003;12(2): 133-137.
  • Chandarana SP, Chanowski EJ, Casper KA, Wolf GT, Bradford CR, Worden FP, Eisbruch A, Chepeha DB. Osteocutaneous free tissue transplantation for mandibular osteoradionecrosis. J Reconstr Microsurg. 2013;29(1): 5-14.
  • Cortellini P, Pini Prato G, Tonetti MS. Interproximal free gingival grafts after membrane removal in guided tissue regeneration treatment of intrabony defects. A randomized controlled clinical trial. J Periodontol. 1995;66(6): 488-493.
  • Deng P, Yao L, Chen J, Tang Z, Zhou J. Chitosan-based hydrogels with injectable, self-healing and antibacterial properties for wound healing. Carbohydr Polym. 2022;276: 118718.
  • Duskova M, Leamerova E, Sosna B, Gojis O. Guided tissue regeneration, barrier membranes and reconstruction of the cleft maxillary alveolus. J Craniofac Surg. 2006;17(6): 1153-1160.
  • Eisenmann DM. Wnt signaling. WormBook. 2005: 1-17.
  • Evans EW. Treating Scars on the Oral Mucosa. Facial Plast Surg Clin North Am. 2017;25(1): 89-97.
  • Fowler EB, Breault LG, Rebitski G. Ridge preservation utilizing an acellular dermal allograft and demineralized freeze-dried bone allograft: Part II. Immediate endosseous implant placement. J Periodontol. 2000;71(8): 1360-1364.
  • Francesko A, Tzanov T. Chitin, chitosan and derivatives for wound healing and tissue engineering. Adv Biochem Eng Biotechnol. 2011;125: 1-27.
  • Guler R, Ercan MT, Ulutuncel N, Devrim H, Uran N. Measurement of blood flow by the 133Xe clearance technique to grafts of amnion used in vestibuloplasty. Br J Oral Maxillofac Surg. 1997;35(4): 280-283.
  • Hakkinen L, Uitto VJ, Larjava H. Cell biology of gingival wound healing. Periodontol 2000. 2000;24: 127-152.
  • Hammad HM, Hammad MM, Abdelhadi IN, Khalifeh MS. Effects of topically applied agents on intra-oral wound healing in a rat model: a clinical and histomorphometric study. Int J Dent Hyg. 2011;9(1): 9-16.
  • Ito M, Yang Z, Andl T, Cui C, Kim N, Millar SE, Cotsarelis G. Wnt-dependent de novo hair follicle regeneration in adult mouse skin after wounding. Nature. 2007;447(7142): 316-320.
  • Jones V, Grey JE, Harding KG. Wound dressings. BMJ. 2006;332(7544): 777-780.
  • Kahnberg KE, Thilander H. Healing of experimental excisional wounds in the rat palate. II. Histological study of electrosurgical wounds. Swed Dent J. 1984;8(2): 49-56.
  • Kozlovsky A, Artzi Z, Hirshberg A, Israeli-Tobias C, Reich L. Effect of local antimicrobial agents on excisional palatal wound healing: a clinical and histomorphometric study in rats. J Clin Periodontol. 2007;34(2): 164-171.
  • Lee HG, Eun HC. Differences between fibroblasts cultured from oral mucosa and normal skin: implication to wound healing. J Dermatol Sci. 1999;21(3): 176-182.
  • Lektemur Alpan A, Calisir M, Kizildag A, Ozdede M, Ozmen O. Effects of a Glycogen Synthase Kinase 3 Inhibitor Tideglusib on Bone Regeneration With Calvarial Defects. J Craniofac Surg. 2020;31(5): 1477-1482.
  • May SR. The effects of biological wound dressings on the healing process. Clin Mater. 1991;8(3-4): 243-249.
  • Minear S, Leucht P, Jiang J, Liu B, Zeng A, Fuerer C, Nusse R, Helms JA. Wnt proteins promote bone regeneration. Sci Transl Med. 2010;2(29): 29ra30.
  • Nakayama A, Kakugo A, Gong JP, Osada Y, Takai M, Erata T. High mechanical strength double-network hydrogel with bacterial cellulose. Advanced Functional Materials. 2004;14(11): 1124-1128.
  • Narang S, Gupta R. Evaluation of resorbable membrane in treatment of human gingival isolated buccal recession. Indian J Dent Res. 2011;22(6): 749-754.
  • Neves VC, Babb R, Chandrasekaran D, Sharpe PT. Promotion of natural tooth repair by small molecule GSK3 antagonists. Sci Rep. 2017;7: 39654.
  • Noh YK, Dos Santos Da Costa A, Park YS, Du P, Kim IH, Park K. Fabrication of bacterial cellulose-collagen composite scaffolds and their osteogenic effect on human mesenchymal stem cells. Carbohydr Polym. 2019;219: 210-218.
  • Oncu B., Yılmaz A.M., Yılmaz B.K., Altunok E.Ç., Kuru L., Ö.B. A. Cytotoxicity and Collagen Expression Effects of Tideglusib Administration on Human Periodontal Cells: An In-Vitro Study. Clinical and Experimental Health Sciences. 2020;10: 153-162.
  • Popelut A, Rooker SM, Leucht P, Medio M, Brunski JB, Helms JA. The acceleration of implant osseointegration by liposomal Wnt3a. Biomaterials. 2010;31(35): 9173-9181.
  • Rastogi S, Modi M, Sathian B. The efficacy of collagen membrane as a biodegradable wound dressing material for surgical defects of oral mucosa: a prospective study. J Oral Maxillofac Surg. 2009;67(8): 1600-1606.
  • Ross P, Mayer R, Benziman M. Cellulose biosynthesis and function in bacteria. Microbiol Rev. 1991;55(1): 35-58.
  • Shanmugam M, Kumar TS, Arun KV, Arun R, Karthik SJ. Clinical and histological evaluation of two dressing materials in the healing of palatal wounds. J Indian Soc Periodontol. 2010;14(4): 241-244.
  • Speroni S, Cicciu M, Maridati P, Grossi GB, Maiorana C. Clinical investigation of mucosal thickness stability after soft tissue grafting around implants: a 3-year retrospective study. Indian J Dent Res. 2010;21(4): 474-479.
  • Tal H. Subgingival acellular dermal matrix allograft for the treatment of gingival recession: a case report. J Periodontol. 1999;70(9): 1118-1124.
  • Tanır HG, Demirezen Ş. Wnt Sinyal Yolunun Biyolojisi ve Bu Yolda Görev Alan Biyomoleküller. Turkiye Klinikleri Journal of Medical Sciences. 2009;29(5): 1292-1297.
  • Taskan MM, Balci Yuce H, Karatas O, Gevrek F, Isiker Kara G, Celt M, Sirma Taskan E. Hyaluronic acid with antioxidants improve wound healing in rats. Biotech Histochem. 2021;96(7): 536-545.
  • UI-Islam M, Shah N, Ha JH, Park J. Effect of chitosan penetration on physico-chemical and mechanical properties of bacterial cellulose. The Korean Journal of Chemical Engineering. 2011;28(8): 1736.
  • Waldrop TC, Semba SE. Closure of oroantral communication using guided tissue regeneration and an absorbable gelatin membrane. J Periodontol. 1993;64(11): 1061-1066.
  • Whyte JL, Smith AA, Helms JA. Wnt signaling and injury repair. Cold Spring Harb Perspect Biol. 2012;4(8): a008078.
  • Zaborowska M, Bodin A, Backdahl H, Popp J, Goldstein A, Gatenholm P. Microporous bacterial cellulose as a potential scaffold for bone regeneration. Acta Biomater. 2010;6(7): 2540-2547.
  • Zhao J, Kim KA, De Vera J, Palencia S, Wagle M, Abo A. R-Spondin1 protects mice from chemotherapy or radiation-induced oral mucositis through the canonical Wnt/beta-catenin pathway. Proc Natl Acad Sci U S A. 2009;106(7): 2331-2336.
Toplam 43 adet kaynakça vardır.

Ayrıntılar

Birincil Dil Türkçe
Konular Diş Hekimliği
Bölüm Özgün Araştırmalar
Yazarlar

Elif Var Odabaş 0000-0002-8541-7504

Semra Ünal Yıldırım 0000-0001-9084-9611

Oğuzhan Gündüz 0000-0002-9427-7574

Berna Alemdağ 0000-0003-4659-5479

Deniz Mukaddes Türet 0000-0003-1989-9637

Ömer Birkan Ağralı 0000-0003-4472-8370

Proje Numarası TDK-2022-10366
Yayımlanma Tarihi 31 Ağustos 2023
Yayımlandığı Sayı Yıl 2023

Kaynak Göster

APA Var Odabaş, E., Ünal Yıldırım, S., Gündüz, O., Alemdağ, B., vd. (2023). Bakteriyel Selüloz Tabanlı Hidrojel Taşıyıcı ile Topikal Uygulanan Tideglusib’in Sıçanlarda Oluşturulan Palatinal Yaranın İyileşmesi Üzerine Etkisinin Makroskobik Değerlendirmesi: Bir Öncül Çalışma. European Journal of Research in Dentistry, 7(2), 72-78. https://doi.org/10.29228/erd.48